Systematic Review: Characteristics and Preclinical Uses of Bladder Cancer Cell Lines

Abstract

Background:Bladder cancer (BC) cell lines are indispensable in basic and preclinical research. Currently, an up-to-date and comprehensive overview of available BC cell lines is not available.Objective:To provide an overview and resources on the origin, pathological and molecular characteristics of commonly used human, murine and canine BC cell lines.Methods:A PubMed search was performed for relevant articles published between 1980 and 2017 according to the following MeSH terms: cell line; cell line, tumor; urinary bladder neoplasms; carcinoma, transitional cell. The Cellosaurus database was searched, using the term “bladder” and/or “urothelial carcinoma”. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.Results:We provide information on 157 human, murine and canine BC cell lines. 103 human BC cell lines have molecular data available, of which 69 have been profiled by at least one “omic” technology. We outline how these cell lines are currently being used for in vitro and in vivo experimental models. These results allow direct comparison of BC cell lines to patient samples, providing information needed to make informed decisions on the most genomically appropriate cell line to answer research questions. Furthermore, we show that cross-contamination remains an issue and describe guidelines for prevention.Conclusions:In the BC field, multiple human, murine and canine BC cell lines have been developed and many have become indispensable for in vitro and in vivo research. High-throughput -omic technologies have dramatically increased the amount of molecular data on these cell lines. We synthesized a comprehensive overview of these data as a resource for the BC scientific community.

GC-MS Metabolomics Reveals Distinct Profiles of Low- and High-Grade Bladder Cancer Cultured Cells

“…The BC cell lines 5637 and J82 were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). Both cell lines were derived from transitional cell carcinoma of the human urinary bladder, with 5637 being classified as grade II and J82 as grade III (stage pT3) [20]. BC cell lines were cultured in MEM to ensure optimal cell growth and maintenance of epithelial cell characteristics.…”

DNA topoisomerase IIα and RAD21 cohesin complex component are predicted as potential therapeutic targets in bladder cancer

“…Meanwhile, the majority of cell lines in the present study (23/26) were derived from a transitional cell carcinoma. On the basis of the invasiveness of the carcinoma, these cell lines were classified into different stages (19). The expression levels of TOP2α mRNA in Stages 1 and 2 were generally lower than those in Stages 3 and 4, indicating that high levels of TOP2α may also be associated with advanced cancer stages (Fig.…”

Pharmaceutical Evaluation of Honokiol and Magnolol on Apoptosis and Migration Inhibition in Human Bladder Cancer Cells

“…However, the T24 and TSGH-8301 cell lines show several distinct characteristics. First, T24 cells were derived from a muscle-invasive tumor with poor differentiation, whereas TSGH-8301 cells are categorized as noninvasive and were derived from a low-grade tumor (Yeh et al, 1988;Zuiverloon et al, 2018). In addition, T24 cells were obtained from a Caucasian female, while TSGH-8301 cells were derived from an Asian male.…”