Systematic evaluation of the gut microbiome of swamp eel (<i>Monopterus albus</i>) by 16S rRNA gene sequencing

Chen, Xuan; Fang, Shouguo; Wei, Lili; Zhong, Qiuyan

doi:10.7717/peerj.8176

Cited by 7 publications

(3 citation statements)

References 60 publications

(70 reference statements)

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“…Probiotics are antagonistic microorganisms of the pathogen, and previous studies suggested that specific Acidobacteria are potential probiotics and that other probiotic additions could also enhance the relative abundance of Acidobacteria (Chen et al, 2019). Our current study revealed that nitrification inhibitor applications increased the relative abundance of special Acidobacteria, which enlightens the possible functional roles of nitrification inhibitors in decreasing the human-disease risks of SMS applications.…”

Section: The Linkages Among Soil Abiotic Properties Microbial Activit...supporting

confidence: 61%

Co‐occurrences of soil nitrogen cycling and human‐disease genes following spent mushroom substrate and nitrification inhibitor applications: A strategy for decreasing health risk

Zhang

Wang

et al. 2022

Land Degrad Dev

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Spent mushroom substrates can be utilized as organic fertilizer in agricultural production but may pose a health risk. Nitrification inhibitors dicyandiamide and 3,4-dimethylpyrazole phosphate have been used to enhance fertilizer nitrogen utilization efficiency. However, the changes of soil abiotic properties, microbial activities, nitrogen cycling and human-disease genes, and their comprehensive relationships after the spent mushroom substrate and nitrification inhibitor applications were still unknown. In this study, the spent mushroom substrate and nitrification inhibitor were applied into the agricultural soils at the rates of 2.5% (w/w) and 1.0% of nitrogen content in the spent mushroom substrate, respectively, and then lettuce was planted. Compared with the control, spent mushroom substrate application significantly increased the lettuce biomass, soil enzyme activities and chitinase, protease, nitric-oxide reductase, nitrogenase, and urease gene abundances. However, the antibiotic resistance and human-disease gene abundances were also significantly enhanced following the spent mushroom substrate application.Relative to spent mushroom substrate alone, the dicyandiamide application significantly decreased the soil pH and human-disease gene abundances, although extra dicyandiamide and 3,4-dimethylpyrazole phosphate enhanced 61.4% and 45.0% of dry biomass of aboveground lettuce. The human-disease gene abundances were positively correlated with soil pH but were negatively correlated with the Acidobacteria and Streptomyces ratios in soils. Spent mushroom substrate application could generate double-edged sword effects, and the health risk should be considered in the spent mushroom substrate applications. Extra dicyandiamide application could further promote crop growth while decrease the human-disease risk from the spent mushroom substrate.

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Section: The Linkages Among Soil Abiotic Properties Microbial Activit...supporting

confidence: 61%

Co‐occurrences of soil nitrogen cycling and human‐disease genes following spent mushroom substrate and nitrification inhibitor applications: A strategy for decreasing health risk

Zhang

Wang

et al. 2022

Land Degrad Dev

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“…In this work, activity of intestinal trypsin enzyme in SBM group were remarkably lower than FM group, dietary soy iso avone and soy saponin and its mixed decreased trypsin. M. albus is a kind of carnivorous aquatic animal [39] , higher activity of trypsin is bene t for protein digestion [24] . These results may explain that dietary soy iso avone and soy saponin decreased growth of M. albus.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary soy isoflavone and soy saponin on growth performance, intestinal structure, intestinal immunity and gut microbiota community on rice field eel (Monopterus albus)

et al. 2021

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Background: Soy iso avone and soy saponin are heat-stable glycosides present in soybean meal, which is the major factor restrict soy protein using in aquatic feed. This study is aimed to conduct that dietary soy iso avone and soy saponin on growth performance, intestinal structure, intestinal immunity and gut microbiota community on rice eld eel (Monopterus albus), and revealed its mechanism. Results: In current study, dietary 1g/kg soy iso avone, 2g/kg soy saponin and their mixed feed decreased growth of M. albus. Dietary 1g/kg soy iso avone signi cantly increased serum alkaline phosphatase (P < 0.05), dietary 2g/kg soy saponin remarkably declined intestinal immunoglobulin M (P < 0.05). Dietary 1g/kg soy iso avone and 2g/kg soy saponin mixed feed signi cantly decreased intestinal complement 3, complement 4 and immunoglobulin M, amounts of intestinal goblet cells per root and shannon of gut microbiota (P < 0.05). Dietary 1g/kg soy iso avone, 2g/kg soy saponin and their mixed feed down regulated intestinal tight junction protein (Interleukin-12, Interleukin-15, Tight junction protein ZO-2-like) and anti-in ammatory (Interleukin-10, Transforming growth factor beta 2) mRNA expression, up regulated pri-in ammatory factors (Interleukin-1 β, Interleukin-6, Interleukin-8, Interleukin-12 and Interleukin-15) mRNA expression. Conclusion: Based on the current results, dietary soy iso avone and soy saponin could damage the intestinal structure and changes gut bacteria community of M. albus, and destroyed the intestinal barrier, eventually induced intestinal in ammatory occurring, soy iso avone and soy saponin mixed feed has more serious effect than they single supplemented.

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“…A potential barrier to the use of microbiome manipulations for sustainable aquaculture is that a signi cant proportion of host-associated bacteria have never been identi ed, in our study and others [15,17,[103][104][105]. For example, one ASV from our study could only be taxonomically ascribed with con dence at the Kingdom level, yet it accounted for > 30% of the total abundance of the microbes in some sh populations.…”

Section: Microbiomes and The Sustainable Development Of Aquaculturementioning

confidence: 66%

Influence of Seaweed Supplements on the Intestinal Bacteria in the Rabbitfish Siganus Fuscescens: Evidence for a Core Microbiome

Thépot

Slinger

Rimmer

et al. 2020

Preprint

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BackgroundWe know very little about natural variation in microbiomes of marine herbivorous fish in the wild or in captivity (aquaculture). Understanding how the consumption of seaweed influences intestinal microbial communities will shed light on how such phytobiotics could enhance the health and productivity of farmed fish. Here we screened the effects of supplementing the diets of mottled rabbitfish (Siganus fuscescens), a candidate species for international aquaculture development, with 15 different species of seaweeds and functional supplements currently used in aquaculture, on the bacterial communities that colonised their hindguts. ResultsRemarkably, the second most abundant phylum and the majority (53%) of the bacterial genera were not assigned, highlighting a significant knowledge gap for the field of animal microbiomes. Dietary supplementation increased alpha diversity by up to 23% relative to the control fish. Furthermore, most supplements significantly increased the relative abundance of Firmicutes, with similar trends for Proteobacteria and consistent decreases in Bacteroides. Seaweed supplementation also had important effects at the genus level, including significant increases in Fusobacterium sp. in fish fed seaweed - especially the green Caulerpa taxifolia – and overall trends for reduced levels of Arcobacter sp., a genus that includes fish and human pathogens. When we compared microbiomes in our fish to those from two recently published studies of conspecific populations sampled many thousands of kilometres away, the populations were clearly distinct, however there were 55 ASVs that were shared across the three fish populations, of which 35 were present in 50% of all fish sampled.ConclusionThe identification of a core microbiome suggests that a host organism relies on certain microbes for key functions and our findings suggest that this candidate aquaculture species has a core microbiome in its hindgut which is robust to dietary manipulations and broad geographical and temporal variation. This insight will help guide future work investigating the functional and mechanistic bases of these relationships, as will improvements in microbial taxonomic resolution. Supplementation with seaweeds did have subtle influences on bacteria in the hindgut of Siganus fuscescens which could have important impacts on fish health and should be considered as aquaculture systems are developed for this species.

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Systematic evaluation of the gut microbiome of swamp eel (Monopterus albus) by 16S rRNA gene sequencing

Cited by 7 publications

References 60 publications

Co‐occurrences of soil nitrogen cycling and human‐disease genes following spent mushroom substrate and nitrification inhibitor applications: A strategy for decreasing health risk

Co‐occurrences of soil nitrogen cycling and human‐disease genes following spent mushroom substrate and nitrification inhibitor applications: A strategy for decreasing health risk

Effects of dietary soy isoflavone and soy saponin on growth performance, intestinal structure, intestinal immunity and gut microbiota community on rice field eel (Monopterus albus)

Influence of Seaweed Supplements on the Intestinal Bacteria in the Rabbitfish Siganus Fuscescens: Evidence for a Core Microbiome

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