Systematic Deviations due to Random Noise Levels in Size Exclusion Chromatography Coupled With Multi Angle Laser Light Scattering

Randomly branched bisphenol A polycarbonates. I. Molecular weight distribution modeling, interfacial synthesis, and characterization

“…The GPC-LS 19,20 results for 3e to g showed reasonably good agreement with the model calculations (Table IX). Each sample gave a high LS signal at the high molecular weight part of the chromatogram, indicative of the presence of highly branched molecules.…”

Interference Prevention in Size-Exclusion Chromatographic Analysis of Debranched Starch Glucans by Aqueous System

“…It became possible to read the absolute molecular weight of low molecular weight molecules, despite the systematic deviation in the low molecular-weight region caused by low light scattering signal. 45 The dn/dc value of amylodextrin (0.147) determined at 690 nm at 35°C on the elution with 0.3 M sodium nitrate is close to 0.146, 17 the value that was determined on the elution with 0.15 M sodium nitrate and has been widely applied in the literature. While this dn/dc value was applied to maltohexaose in the current system, the difference between calculated and theoretical molecular weight was less than 10%.…”

Viscosimetric detection in size‐exclusion chromatography (SEC/GPC): The Goldwasser method and beyond

“…In addition to baseline determination [24,25], the injection concentration is thus an important parameter to optimize in order to use the Goldwasser method without compromising the possibility to determine distributions. Signal-to-noise ratio is known to be an important parameter in multiple-detection SEC [11,20]. It also plays a role in the single-detector method that the Goldwasser method is.…”

“…Labelling is possible [18,19], but this additional step is generally not used. Low signal-to-noise ratio as obtained with the refractive index detector are however not desirable, since it can lead to important artefacts in the determination of molecular weight by multiple-detection SEC [11,20]. Finally, industrial polymers are generally copolymers so that they can combine different properties.…”

“…There are however a number of limitations. A general limitation is that the difference in sensitivity of the detectors can lead to some artefacts where the signal-to-noise is too weak on one of the detectors [11]. A specific limitation is that a number of important polymers have a refractive index close to the one of common eluents.…”