Systematic assessment of chlorine tolerance mechanism in a potent biofilm-forming marine bacterium Halomonas boliviensis

Rajeev, Meora; Sushmitha, T.J.; Prasath, Krishnan Ganesh; Toleti, Subba Rao; Pandian, Shunmugiah Karutha

doi:10.1016/j.ibiod.2020.104967

Cited by 21 publications

(8 citation statements)

References 44 publications

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“…Field sampling was conducted in the intake area of a nuclear power plant (08°10′08′′N, 77°42′45′′E) located in the coastal region of Kudankulam, Tamil Nadu, India ( S1a Fig in S1 File ). A detailed description of the studied power plant is provided in our previous study [ 12 ]. The study site is located in the distal end of the Gulf of Mannar Biosphere Reserve and is endowed with rich marine diversity with various species of microbes, corals, fishes, crustaceans, mollusks, and sponges [ 37 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…The development of the microbial growth on acrylic coupons was visualized using field-emission scanning electron microscope (FESEM, SUPRA 55VP; Carl Zeiss, Germany) as described in our previous study [ 12 ]. Briefly, the bacterial biomass fixed on the acrylic coupons was dehydrated by passing through the graded ethanol concentrations (20, 40, 60, 80, and 100%; 10 min each).…”

Section: Methodsmentioning

confidence: 99%

“…Coastal positioned cooling systems utilize a large volume of seawater as a key source for waste heat removal [ 11 , 12 ]. The traversing seawater through power plants generally contains a wide spectrum of microorganisms that tend to settle and form biofilms on the surface of the condenser and other operational machinery [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Bacterial community structure of early-stage biofilms is dictated by temporal succession rather than substrate types in the southern coastal seawater of India

et al. 2021

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Bacterial communities colonized on submerged substrata are recognized as a key factor in the formation of complex biofouling phenomenon in the marine environment. Despite massive maritime activities and a large industrial sector in the nearshore of the Laccadive Sea, studies describing pioneer bacterial colonizers and community succession during the early-stage biofilm are scarce. We investigated the biofilm-forming bacterial community succession on three substrata viz. stainless steel, high-density polyethylene, and titanium over 15 days of immersion in the seawater intake area of a power plant, located in the southern coastal region of India. The bacterial community composition of biofilms and peripheral seawater were analyzed by Illumina MiSeq sequenced 16S rRNA gene amplicons. The obtained metataxonomic results indicated a profound influence of temporal succession over substrate type on the early-stage biofilm-forming microbiota. Bacterial communities showed vivid temporal dynamics that involved variations in abundant bacterial groups. The proportion of dominant phyla viz. Proteobacteria decreased over biofilm succession days, while Bacteroidetes increased, suggesting their role as initial and late colonizers, respectively. A rapid fluctuation in the proportion of two bacterial orders viz. Alteromonadales and Vibrionales were observed throughout the successional stages. LEfSe analysis identified specific bacterial groups at all stages of biofilm development, whereas no substrata type-specific groups were observed. Furthermore, the results of PCoA and UPGMA hierarchical clustering demonstrated that the biofilm-forming community varied considerably from the planktonic community. Phylum Proteobacteria preponderated the biofilm-forming community, while the Bacteroidetes, Cyanobacteria, and Actinobacteria dominated the planktonic community. Overall, our results refute the common assumption that substrate material has a decisive impact on biofilm formation; rather, it portrayed that the temporal succession overshadowed the influence of the substrate material. Our findings provide a scientific understanding of the factors shaping initial biofilm development in the marine environment and will help in designing efficient site-specific anti-biofouling strategies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Bacterial community structure of early-stage biofilms is dictated by temporal succession rather than substrate types in the southern coastal seawater of India

et al. 2021

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“…In addition, some species of this genus, viz. , P. ruthenica and P. espejiana , have been shown to produce dense exopolysaccharide (EPS) that helps them in forming potent biofilms on artificial surfaces ( 6 – 8 ).…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequencing of Pseudoalteromonas tetraodonis Strain kknpp56, a Potent Biofilm-Forming Bacterium Isolated from Early-Stage Marine Biofilm

Rajeev

Sushmitha

Toleti

et al. 2021

Microbiol Resour Announc

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“…Marine biofouling is the attachment of undesirable molecules and micro-and macroorganisms to submerged surfaces, posing serious economic and environmental implications. Biofouling on ship hulls causes an increase in frictional drag, which leads to higher fuel consumption, maintenance costs, and downtimes [1][2][3]. Moreover, submerged marine facilities and equipment can be damaged by biofouling, representing additional costs for marine industries [1].…”

Section: Introductionmentioning

confidence: 99%

Unveiling the Antifouling Performance of Different Marine Surfaces and Their Effect on the Development and Structure of Cyanobacterial Biofilms

et al. 2021

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Since biofilm formation by microfoulers significantly contributes to the fouling process, it is important to evaluate the performance of marine surfaces to prevent biofilm formation, as well as understand their interactions with microfoulers and how these affect biofilm development and structure. In this study, the long-term performance of five surface materials—glass, perspex, polystyrene, epoxy-coated glass, and a silicone hydrogel coating—in inhibiting biofilm formation by cyanobacteria was evaluated. For this purpose, cyanobacterial biofilms were developed under controlled hydrodynamic conditions typically found in marine environments, and the biofilm cell number, wet weight, chlorophyll a content, and biofilm thickness and structure were assessed after 49 days. In order to obtain more insight into the effect of surface properties on biofilm formation, they were characterized concerning their hydrophobicity and roughness. Results demonstrated that silicone hydrogel surfaces were effective in inhibiting cyanobacterial biofilm formation. In fact, biofilms formed on these surfaces showed a lower number of biofilm cells, chlorophyll a content, biofilm thickness, and percentage and size of biofilm empty spaces compared to remaining surfaces. Additionally, our results demonstrated that the surface properties, together with the features of the fouling microorganisms, have a considerable impact on marine biofouling potential.

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Systematic assessment of chlorine tolerance mechanism in a potent biofilm-forming marine bacterium Halomonas boliviensis

Cited by 21 publications

References 44 publications

Bacterial community structure of early-stage biofilms is dictated by temporal succession rather than substrate types in the southern coastal seawater of India

Bacterial community structure of early-stage biofilms is dictated by temporal succession rather than substrate types in the southern coastal seawater of India

Draft Genome Sequencing of Pseudoalteromonas tetraodonis Strain kknpp56, a Potent Biofilm-Forming Bacterium Isolated from Early-Stage Marine Biofilm

Unveiling the Antifouling Performance of Different Marine Surfaces and Their Effect on the Development and Structure of Cyanobacterial Biofilms

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