Unveiling the Antifouling Performance of Different Marine Surfaces and Their Effect on the Development and Structure of Cyanobacterial Biofilms

Faria, Sara; Teixeira‐Santos, Rita; Romeu, Maria J.; Morais, João; Jong, Ed de; Sjollema, Jelmer; Vasconcelos, Vı́tor; Mergulhão, Filipe

doi:10.3390/microorganisms9051102

Cited by 17 publications

(17 citation statements)

References 65 publications

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“…Moreover, one of the key issues in the development of new AF surfaces is the necessary screening of candidate surfaces (usually tested in vitro in a first step prior to in situ testing) before scale-up and final performance evaluation. Since it has been shown that hydrodynamics can severely affect biofilm formation [ 87 , 88 , 89 , 90 ] and gene expression by fouling organisms [ 91 , 92 ], it is recommended that these tests be performed in controlled hydrodynamic conditions that mimic the final application scenario [ 93 ].…”

Section: Resultsmentioning

confidence: 99%

Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Review

2022

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Although carbon materials are widely used in surface engineering, particularly graphene (GP) and carbon nanotubes (CNTs), the application of these nanocomposites for the development of antibiofilm marine surfaces is still poorly documented. The aim of this study was, thus, to gather and discuss the relevant literature concerning the antifouling performance of carbon-based coatings against marine micro- and macrofoulers. For this purpose, a PRISMA-oriented systematic review was conducted based on predefined criteria, which resulted in the selection of thirty studies for a qualitative synthesis. In addition, the retrieved publications were subjected to a quality assessment process based on an adapted Methodological Index for Non-Randomized Studies (MINORS) scale. In general, this review demonstrated the promising antifouling performance of these carbon nanomaterials in marine environments. Further, results from the revised studies suggested that functionalized GP- and CNTs-based marine coatings exhibited improved antifouling performance compared to these materials in pristine forms. Thanks to their high self-cleaning and enhanced antimicrobial properties, as well as durability, these functionalized composites showed outstanding results in protecting submerged surfaces from the settlement of fouling organisms in marine settings. Overall, these findings can pave the way for the development of new carbon-engineered surfaces capable of preventing marine biofouling.

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Section: Resultsmentioning

confidence: 99%

Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Review

2022

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“…These devices are easy to handle, which allows for studying the adhesion of different microbial strains and consequent biofilm formation in rapid and inexpensive assays, due to their reduced volume [ 69 ]. Depending on the format used, they enable high throughput at an affordable cost and sometimes non-invasive imaging through optical coherence tomography (OCT) [ 70 , 71 ] and confocal laser scanning microscopy (CLSM) [ 72 ]. Particularly for larger well dimensions, it is possible to place coupons at the bottom of the wells so that different surface materials can be tested [ 70 , 73 , 74 ].…”

Section: Biofilm Platformsmentioning

confidence: 99%

“…Depending on the format used, they enable high throughput at an affordable cost and sometimes non-invasive imaging through optical coherence tomography (OCT) [ 70 , 71 ] and confocal laser scanning microscopy (CLSM) [ 72 ]. Particularly for larger well dimensions, it is possible to place coupons at the bottom of the wells so that different surface materials can be tested [ 70 , 73 , 74 ]. The main limitations of microplates are that loosely attached cells may not be measured correctly due to detachment during washing and that biofilms formed in this platform are affected by sedimentation.…”

Section: Biofilm Platformsmentioning

confidence: 99%

“…However, in the last few years, efforts have been made to predict flow conditions in easy-to-handle biofilm platforms like microplates [ 68 , 71 ]. A detailed hydrodynamic analysis of the 12-well microplates [ 71 ] allows us to define the operational conditions that should be used in the laboratory bench to further assess the biofilm formation capacity of marine bacteria [ 70 , 71 ] and the antibiofilm activity of novel surface coatings [ 22 , 82 ] under hydrodynamic conditions prevailing in natural aquatic environments.…”

Section: Adhesion and Biofilm Studies Performed Under Controlled Hydrodynamicsmentioning

confidence: 99%

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A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions

Gomes

Mergulhão

2021

Microorganisms

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The early colonization of surfaces and subsequent biofilm development have severe impacts in environmental, industrial, and biomedical settings since they entail high costs and health risks. To develop more effective biofilm control strategies, there is a need to obtain laboratory biofilms that resemble those found in natural or man-made settings. Since microbial adhesion and biofilm formation are strongly affected by hydrodynamics, the knowledge of flow characteristics in different marine, food processing, and medical device locations is essential. Once the hydrodynamic conditions are known, platforms for cell adhesion and biofilm formation should be selected and operated, in order to obtain reproducible biofilms that mimic those found in target scenarios. This review focuses on the most widely used platforms that enable the study of initial microbial adhesion and biofilm formation under controlled hydrodynamic conditions - modified Robbins devices, flow chambers, rotating biofilm devices, microplates, and microfluidic devices - and where numerical simulations have been used to define relevant flow characteristics, namely the shear stress and shear rate.

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“…Although cyanobacteria proteomics has dealt with many technical challenges 22 , cyanobacteria molecular biology is a promising field for further advancements 23 , 24 . The fouling surface properties and shear rate are two parameters that have a critical impact on marine biofilms 25 , 26 . In a previous qualitative proteomic study, the proteomic profile of biofilm cells from two cyanobacterial strains developed on glass and perspex, and at different shear forces was analysed 27 .…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic conditions affect the proteomic profile of marine biofilms formed by filamentous cyanobacterium

Romeu

Domínguez-Pérez

Almeida

et al. 2022

npj Biofilms Microbiomes

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Proteomic studies on cyanobacterial biofilms can be an effective approach to unravel metabolic pathways involved in biofilm formation and, consequently, obtain more efficient biofouling control strategies. Biofilm development by the filamentous cyanobacterium Toxifilum sp. LEGE 06021 was evaluated on different surfaces, glass and perspex, and at two significant shear rates for marine environments (4 s−1 and 40 s−1). Higher biofilm development was observed at 4 s−1. Overall, about 1877 proteins were identified, and differences in proteome were more noticeable between hydrodynamic conditions than those found between surfaces. Twenty Differentially Expressed Proteins (DEPs) were found between 4 s−1 vs. 40 s−1. On glass, some of these DEPs include phage tail proteins, a carotenoid protein, cyanophynase glutathione-dependent formaldehyde dehydrogenase, and the MoaD/ThiS family protein, while on perspex, DEPs include transketolase, dihydroxy-acid dehydratase, iron ABC transporter substrate-binding protein and protein NusG. This study contributes to developing a standardized protocol for proteomic analysis of filamentous cyanobacterial biofilms. This kind of proteomic analysis can also be useful for different research fields, given the broad spectrum of promising secondary metabolites and added-value compounds produced by cyanobacteria, as well as for the development of new antibiofilm strategies.

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Unveiling the Antifouling Performance of Different Marine Surfaces and Their Effect on the Development and Structure of Cyanobacterial Biofilms

Cited by 17 publications

References 65 publications

Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Review

Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Review

A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions

Hydrodynamic conditions affect the proteomic profile of marine biofilms formed by filamentous cyanobacterium

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