Systematic Analysis of Gibberellin Pathway Components in Medicago truncatula Reveals the Potential Application of Gibberellin in Biomass Improvement

Wang, Hongfeng; Jiang, Hongjiao; Xu, Yiteng; Wang, Yan; Zhu, Lin; Yu, Xiaolin; Kong, Fanjiang; Zhou, Chuanen; Han, Li

doi:10.3390/ijms21197180

Cited by 10 publications

(6 citation statements)

References 80 publications

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“…Similarity, in M. truncatula , loss of DWARF AND INCREASED BRANCHING 1 ( DIB1 ) (syn. MtGA3OX1 ) function also leads plants to produce small leaves due to decreased cell length, whereas plants over‐expressing MtGA20ox1 produce larger leaves, implying the mechanism by which GA regulates leaf size is conserved among different species (Wang et al, 2020; Zhang et al, 2020a). Meanwhile, BR promotes leaf growth via its positive effect on both cell proliferation and cell expansion.…”

Section: The Regulation Of Leaf Size and Growthmentioning

confidence: 99%

From genes to networks: The genetic control of leaf development

Wang

Kong

Zhou

2021

JIPB

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Substantial diversity exists for both the size and shape of the leaf, the main photosynthetic organ of flowering plants. The two major forms of leaf are simple leaves, in which the leaf blade is undivided, and compound leaves, which comprise several leaflets. Leaves form at the shoot apical meristem from a group of undifferentiated cells, which first establish polarity, then grow and differentiate. Each of these processes is controlled by a combination of transcriptional regulators, microRNAs and phytohormones. The present review documents recent advances in our understanding of how these various factors modulate the development of both simple leaves (focusing mainly on the model plant Arabidopsis thaliana) and compound leaves (focusing mainly on the model legume species Medicago truncatula).

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Section: The Regulation Of Leaf Size and Growthmentioning

confidence: 99%

From genes to networks: The genetic control of leaf development

Wang

Kong

Zhou

2021

JIPB

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“…Studies on several dwarf mutants in pea ( Pisum sativum ), soybean ( Glycine max ), and the model legume M. truncatula have suggested that the GA pathway plays a conserved role in controlling plant height in legumes ( Yaxley et al, 2001 ; Li et al, 2018 ; Guo et al, 2020 ; Zhang et al, 2020 ). Notably, despite the M. truncatula GA20ox family genes exhibited functional redundancy ( Ma et al, 2019 ), overexpression of GA20ox can increase M. truncatula plant height and biomass ( Wang et al, 2020 ), suggesting an application potential of the GA20ox family genes for biomass improvement in legumes. Nevertheless, due to the ancient genome duplication event, most legume plants undergo gene duplication and subsequent functional diversification ( Shoemaker et al, 2006 ), thus the biological functions of the GA20ox family genes in legumes remain largely unclear.…”

Section: Introductionmentioning

confidence: 99%

The GA 20-Oxidase Encoding Gene MSD1 Controls the Main Stem Elongation in Medicago truncatula

Yin

et al. 2021

Front. Plant Sci.

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Plant height is an important agronomic trait that is closely related to biomass yield and crop production. Despite legumes comprise one of the largest monophyletic families that are second only to grasses in terms of economic and nutritional values, due to an ancient genome duplication event, most legume plants have complex genomes, thus the molecular mechanisms that determine plant height are less known in legumes. Here, we report the identification and characterization of MAIN STEM DWARF1 (MSD1), which is required for the plant height in the model legume Medicago truncatula. Loss of function of MSD1 leads to severely reduced main stem height but normal lateral branch elongation in M. truncatula. Histological analysis revealed that the msd1-1 main stem has shorter internodes with reduced cell size and number compared with the wild type, indicating that MSD1 affects cell elongation and cell proliferation. MSD1 encodes a putative GA 20-oxidase that is expressed at significantly higher levels in the main shoot apex than in the lateral shoot apices, suggesting that MSD1 expression is associated with its effect on the main stem elongation. UPLC-MS/MS analysis showed that GA9 and GA4, two identified products of the GA 20-oxidase, were severely reduced in msd1-1, and the dwarf phenotype of msd1-1 could be rescued by supplementation with gibberellic acid GA3, confirming that MSD1 functions as a biologically active GA 20-oxidase. Moreover, we found that disruption of either MtGA20ox7 or MtGA20ox8, homologs of MSD1, has little effects on the elongation of the main stem, while the msd1-1 mtga20ox7-1 mtga20ox8 triple mutants exhibits a severe short main shoot and lateral branches, as well as reduced leaf size, suggesting that MSD1 and its homologs MtGA20ox7 and MtGA20ox8, redundantly regulate M. truncatula shoot elongation and leaf development. Taken together, our findings demonstrate the molecular mechanism of MSD1-mediated regulation of main stem elongation in M. truncatula and provide insights into understanding the functional diversity of GA 20-oxidases in optimizing plant architecture in legumes.

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“…GID1 and DELLA are also present and interact in M. truncatula (Jiao et al 2020 ). One of the candidates that we identified was a gene similar to M. truncatula GIBBERELIN-INSENSITIVE 1C (GID1C) (Wang et al 2020b ) , located in 1_12.58. A gene similar to the INDETERMINATE DOMAIN (IDD) group of the C2H2 zinc finger protein family was located in 7_14.52.…”

Section: Discussionmentioning

confidence: 99%

Identification of loci controlling timing of stem elongation in red clover using genotyping by sequencing of pooled phenotypic extremes

et al. 2022

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Main conclusion Through selective genotyping of pooled phenotypic extremes, we identified a number of loci and candidate genes putatively controlling timing of stem elongation in red clover. Abstract We have identified candidate genes controlling the timing of stem elongation prior to flowering in red clover (Trifolium pratense L.). This trait is of ecological and agronomic significance, as it affects fitness, competitivity, climate adaptation, forage and seed yield, and forage quality. We genotyped replicate pools of phenotypically extreme individuals (early and late-elongating) within cultivar Lea using genotyping-by-sequencing in pools (pool-GBS). After calling and filtering SNPs and GBS locus haplotype polymorphisms, we estimated allele frequencies and searched for markers with significantly different allele frequencies in the two phenotypic groups using BayeScan, an FST-based test utilizing replicate pools, and a test based on error variance of replicate pools. Of the three methods, BayeScan was the least stringent, and the error variance-based test the most stringent. Fifteen significant markers were identified in common by all three tests. The candidate genes flanking the markers include genes with potential roles in the vernalization, autonomous, and photoperiod regulation of floral transition, hormonal regulation of stem elongation, and cell growth. These results provide a first insight into the potential genes and mechanisms controlling transition to stem elongation in a perennial legume, which lays a foundation for further functional studies of the genetic determinants regulating this important trait.

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Systematic Analysis of Gibberellin Pathway Components in Medicago truncatula Reveals the Potential Application of Gibberellin in Biomass Improvement

Cited by 10 publications

References 80 publications

From genes to networks: The genetic control of leaf development

From genes to networks: The genetic control of leaf development

The GA 20-Oxidase Encoding Gene MSD1 Controls the Main Stem Elongation in Medicago truncatula

Identification of loci controlling timing of stem elongation in red clover using genotyping by sequencing of pooled phenotypic extremes

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