Syringe pumped high speed flow cytometry of oceanic phytoplankton

Zubkov, Mikhail V.; Burkill, Peter H.

doi:10.1002/cyto.a.20332

Cited by 74 publications

(46 citation statements)

References 43 publications

(73 reference statements)

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“…Previous work has shown no loss of either prokaryotic or eukaryotic cells occurs upon fixation (Zubkov and Burkill, 2006). Synechococcus cells were counted unstained (Zubkov et al, 2000) while eukaryotic phytoplankton and Prochlorococcus were counted after staining with SYBR Green I (Sigma-Aldrich, 1:5000 dilution of the commercial stock; see Zubkov et al, 2000Zubkov et al, , 2007.…”

Section: Methodsmentioning

confidence: 99%

Significant CO2 fixation by small prymnesiophytes in the subtropical and tropical northeast Atlantic Ocean

et al. 2010

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Global estimates indicate the oceans are responsible for approximately half of the carbon dioxide fixed on Earth. Organisms p5 lm in size dominate open ocean phytoplankton communities in terms of abundance and CO 2 fixation, with the cyanobacterial genera Prochlorococcus and Synechococcus numerically the most abundant and more extensively studied compared with small eukaryotes. However, the contribution of specific taxonomic groups to marine CO 2 fixation is still poorly known. In this study, we show that among the phytoplankton, small eukaryotes contribute significantly to CO 2 fixation (44%) because of their larger cell volume and thereby higher cell-specific CO 2 fixation rates. Within the eukaryotes, two groups, herein called Euk-A and Euk-B, were distinguished based on their flow cytometric signature. Euk-A, the most abundant group, contained cells 1.8±0.1 lm in size while Euk-B was the least abundant but cells were larger (2.8±0.2 lm). The Euk-B group comprising prymnesiophytes (73±13%) belonging largely to lineages with no close cultured counterparts accounted for up to 38% of the total primary production in the subtropical and tropical northeast Atlantic Ocean, suggesting a key role of this group in oceanic CO 2 fixation.

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Section: Methodsmentioning

confidence: 99%

Significant CO2 fixation by small prymnesiophytes in the subtropical and tropical northeast Atlantic Ocean

et al. 2010

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“…Marie et al, 1997). An internal standard of 0.5 and 1.0 µm beads (Fluoresbrite microparticles, Polysciences), the concentration of which was determined by syringe pump flow cytometry (Zubkov and Burkill, 2006b), was added to each of the samples. Bacterioplankton groups were distinguished according to their DNA content and scatter properties.…”

Section: Bacterioplanktonmentioning

confidence: 99%

Photoheterotrophy of bacterioplankton is ubiquitous in the surface oligotrophic ocean

Evans

Gómez-Pereira

Martin

et al. 2015

Progress in Oceanography

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Accurate measurements in the Southern Hemisphere were required to test a hypothesis of the ubiquity of photoheterotrophy in the oligotrophic ocean. We present experimental results of light-enhanced uptake of methionine, leucine and ATP by bacterioplankton during two large-scale transects of the South Atlantic. Light increased the uptake of substrates by both dominant bacterioplankton groups: Prochlorococcus and SAR11, as well as for the bulk microbial community. Our consistent experimental evidence strongly indicates that photoheterotrophy is characteristic of dominant bacterioplankton populations in the global oligotrophic ocean.

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“…Yellow-green 0.5 and 1.0 mm reference beads (Fluoresbrite Microparticles, Polysciences, Warrington, PA, USA) were used in all analyses as an internal standard for both fluorescence and flow rates. The absolute concentration of beads in the stock solution was determined using syringe pump flow cytometry (Zubkov and Burkill, 2006).…”

Section: Sampling and Bacterioplankton Enumerationmentioning

confidence: 99%

Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre

et al. 2012

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Subtropical oceanic gyres are the most extensive biomes on Earth where SAR11 and Prochlorococcus bacterioplankton numerically dominate the surface waters depleted in inorganic macronutrients as well as in dissolved organic matter. In such nutrient poor conditions bacterioplankton could become photoheterotrophic, that is, potentially enhance uptake of scarce organic molecules using the available solar radiation to energise appropriate transport systems. Here, we assessed the photoheterotrophy of the key microbial taxa in the North Atlantic oligotrophic gyre and adjacent regions using 33 P-ATP, 3 H-ATP and 35 S-methionine tracers. Light-stimulated uptake of these substrates was assessed in two dominant bacterioplankton groups discriminated by flow cytometric sorting of tracer-labelled cells and identified using catalysed reporter deposition fluorescence in situ hybridisation. One group of cells, encompassing 48% of all bacterioplankton, were identified as members of the SAR11 clade, whereas the other group (24% of all bacterioplankton) was Prochlorococcus. When exposed to light, SAR11 cells took 31% more ATP and 32% more methionine, whereas the Prochlorococcus cells took 33% more ATP and 34% more methionine. Other bacterioplankton did not demonstrate light stimulation. Thus, the SAR11 and Prochlorococcus groups, with distinctly different light-harvesting mechanisms, used light equally to enhance, by approximately one-third, the uptake of different types of organic molecules. Our findings indicate the significance of light-driven uptake of essential organic nutrients by the dominant bacterioplankton groups in the surface waters of one of the less productive, vast regions of the world's oceans-the oligotrophic North Atlantic subtropical gyre.

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Syringe pumped high speed flow cytometry of oceanic phytoplankton

Cited by 74 publications

References 43 publications

Significant CO2 fixation by small prymnesiophytes in the subtropical and tropical northeast Atlantic Ocean

Significant CO2 fixation by small prymnesiophytes in the subtropical and tropical northeast Atlantic Ocean

Photoheterotrophy of bacterioplankton is ubiquitous in the surface oligotrophic ocean

Comparable light stimulation of organic nutrient uptake by SAR11 and Prochlorococcus in the North Atlantic subtropical gyre

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