Synuclein-γ promotes migration of MCF7 breast cancer cells by activating extracellular-signal regulated kinase pathway and breaking cell-cell junctions

Zhuang, Qing; Liu, Caiyun; Qu, Like; Shou, Chengchao

doi:10.3892/mmr.2015.3799

Cited by 11 publications

(15 citation statements)

References 26 publications

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“…In migration assays, SNCG silencing significantly decreased cell migration in cell lines from both ECC and ICC. This finding was consistent with previous reports in breast cancer, hepatocellular carcinoma, and gallbladder carcinoma 38–40 . The results of in vitro assays suggested that the role of SNCG may be associated with cell migration, which is generally linked to cell differentiation and metastasis regardless of the origin of the biliary tract tumor.…”

Section: Discussionsupporting

confidence: 92%

“…It has been reported that SNCG increases cell proliferation in gallbladder, colorectal, and prostate cancer 30,38,41 . However, other studies on breast cancer and pancreatic cancer showed no significant correlation between SNCG expression and cell proliferation 9,39 . These inconsistencies may result from differences in the functions of SNCG expression in each cancer.…”

Section: Discussionmentioning

confidence: 99%

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Gamma‐synuclein is a novel prognostic marker that promotes tumor cell migration in biliary tract carcinoma

et al. 2021

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Gamma‐synuclein (SNCG) promotes invasive behavior and is reportedly a prognostic factor in a range of cancers. However, its role in biliary tract carcinoma (BTC) remains unknown. Consequently, we investigated the clinicopathological significance and function of SNCG in BTC. Using resected BTC specimens from 147 patients with adenocarcinoma (extrahepatic cholangiocarcinoma [ECC, n = 96]; intrahepatic cholangiocarcinoma [ICC, n = 51]), we immunohistochemically evaluated SNCG expression and investigated its correlation with clinicopathological factors and outcomes. Furthermore, cell lines with high SNCG expression were selected from 16 BTC cell lines and these underwent cell proliferation and migration assays by siRNAs. In the results, SNCG expression was present in 22 of 96 (22.9%) ECC patients and in 10 of 51 (19.6%) ICC patients. SNCG expression was significantly correlated with poorly differentiated tumor in both ECC and ICC (p = 0.01 and 0.03, respectively) and with perineural invasion and lymph node metastases in ECC (p = 0.04 and 0.003, respectively). Multivariate analyses revealed that SNCG expression was an independent poor prognostic factor in both OS and RFS in both ECC and ICC. In vitro analyses showed high SNCG expression in three BTC cell lines (NCC‐BD1, NCC‐BD3, and NCC‐CC6‐1). Functional analysis revealed that SNCG silencing could suppress cell migration in NCC‐BD1 and NCC‐CC6‐1 and downregulate cell proliferation in NCC‐CC6‐1 significantly. In conclusion, SNCG may promote tumor cell activity and is potentially a novel prognostic marker in BTC.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Gamma‐synuclein is a novel prognostic marker that promotes tumor cell migration in biliary tract carcinoma

et al. 2021

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“…In addition, it is expressed at presynaptic terminals in the brain and the peripheral nervous system (35). It promotes migration, invasion and metastasis of tumor cells (36), which have proven that expression of SNCG protein is elevated in the advanced stages of various types of cancers, including ovarian (37), lung, liver (38), esophagus (39), colon (38) and in particular, breast (40,41). For example, Singh and Jia (41) revealed that SNCG protein expression was correlated with pathological factors, including lymph node metastasis, tumor size and stage, and follow-up revealed that the survival rate of patients with SNCG -positive protein expression were significantly lower than the patients with negative expression for 438 cases with breast cancer.…”

Section: Discussionmentioning

confidence: 99%

Combining affinity propagation clustering and mutual information network to investigate key genes in fibroid

Chen

Wang

Liu

et al. 2017

Experimental and Therapeutic Medicine

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The aim of the present study was to investigate key genes in fibroids based on the multiple affinity propogation-Krzanowski and Lai (mAP-KL) method, which included the maxT multiple hypothesis, Krzanowski and Lai (KL) cluster quality index, affinity propagation (AP) clustering algorithm and mutual information network (MIN) constructed by the context likelihood of relatedness (CLR) algorithm. In order to achieve this goal, mAP-KL was initially implemented to investigate exemplars in fibroid, and the maxT function was employed to rank the genes of training and test sets, and the top 200 genes were obtained for further study. In addition, the KL cluster index was applied to determine the quantity of clusters and the AP clustering algorithm was conducted to identify the clusters and their exemplars. Subsequently, the support vector machine (SVM) model was selected to evaluate the classification performance of mAP-KL. Finally, topological properties (degree, closeness, betweenness and transitivity) of exemplars in MIN constructed according to the CLR algorithm were assessed to investigate key genes in fibroid. The SVM model validated that the classification between normal controls and fibroid patients by mAP-KL had a good performance. A total of 9 clusters and exemplars were identified based on mAP-KL, which were comprised of CALCOCO2, COL4A2, COPS8, SNCG, PA2G4, C17orf70, MARK3, BTNL3 and TBC1D13. By accessing the topological analysis for exemplars in MIN, SNCG and COL4A2 were identified as the two most significant genes of four types of methods, and they were denoted as key genes in the progress of fibroid. In conclusion, two key genes (SNCG and COL4A2) and 9 exemplars were successfully investigated, and these may be potential biomarkers for the detection and treatment of fibroid.

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“…The pro-invasive capacity of α-synuclein provides a biological explanation for its upregulation in atypical and anaplastic meningiomas. Although there is few evidence for α-synuclein-mediated cell invasiveness, γ-synuclein has been shown to promote cell migration and invasion in different types of tumor cells such as oral squamous cell carcinoma [12], breast cancer [21], and gastric cancer [22]. …”

Section: Discussionmentioning

confidence: 99%

Alpha-synuclein contributes to malignant progression of human meningioma via the Akt/mTOR pathway

2016

Cancer Cell Int

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BackgroundThe aim of this study is to explore the expression of alpha-synuclein (α-synuclein) in benign, atypical, and anaplastic meningiomas and determine its role in the malignant progression of meningiomas.MethodsExpression of α-synuclein was measured in 44 meningioma samples by real-time PCR analysis. The effects of overexpression or knockdown of α-synuclein on meningioma cell growth, invasiveness, and tumorigenicity were determined.ResultsAtypical and anaplastic meningiomas displayed significantly greater levels of α-synuclein mRNA, relative to benign tumors. Depletion of α-synuclein decreased cell proliferation and colony formation and promoted apoptosis in IOMM-Lee meningioma cells, whereas overexpression of α-synuclein facilitated cell proliferation and colony formation in CH-157MN meningioma cells. Silencing of α-synuclein attenuated IOMM-Lee cell migration and invasion. In contrast, ectopic expression of α-synuclein increased the invasiveness of CH-157MN cells. In vivo studies further demonstrated that downregulation of α-synuclein significantly retarded meningioma growth in nude mice. At the molecular level, the phosphorylation levels of Akt, mTOR, p70S6K and 4EBP were significantly decreased in α-synuclein-depleted IOMM-Lee cells.ConclusionsIn conclusion, α-synuclein upregulation contributes to aggressive phenotypes of meningiomas via the Akt/mTOR pathway and thus represents a potential therapeutic target for malignant meningiomas.

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Synuclein-γ promotes migration of MCF7 breast cancer cells by activating extracellular-signal regulated kinase pathway and breaking cell-cell junctions

Cited by 11 publications

References 26 publications

Gamma‐synuclein is a novel prognostic marker that promotes tumor cell migration in biliary tract carcinoma

Gamma‐synuclein is a novel prognostic marker that promotes tumor cell migration in biliary tract carcinoma

Combining affinity propagation clustering and mutual information network to investigate key genes in fibroid

Alpha-synuclein contributes to malignant progression of human meningioma via the Akt/mTOR pathway

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