Synthetic studies on the carbohydrate moiety of the antigen from the parasite Echinococcusmultilocularis

Echinococcus multilocularisand Its Intermediate Host: A Model of Parasite-Host Interplay

“…Hülsmeier and co-workers [33] have then isolated novel mucin-type glycoforms from the metacestode of E. multilocularis , and these glycoforms contained mucin-type core-I type and core-II type structures that were further diversified by addition of GlcNAc or Gal residues. Koizumi et al [34] reported on the synthesis of the glycan portions of a glycoprotein antigen of E. multilocularis in order to elucidate the interactions between oligosaccharides and sera of E. multilocularis -infected hosts. Stereocontrolled synthesis of branched tri-, tetra-, and pentasaccharides displaying a Gal β 1->3GalNAc core in the glycan portion of the glycoprotein antigen was achieved, which may become an interesting tool for further studies on their putative biological function.…”

Galα1-4Galβ1-3GalNAc is the dominant epitope of Em2 antigen, the mucin-type glycoprotein from Echinococcus multilocularis

“…Significant differences were observed between the AE patient group and NH group (P < 0.001, Student's t test). Among the 11 oligosaccharides we have synthesized (Koizumi et al 2009;2011), only the three in Table 1 showed strong reactivity, and the other eight showed only a low sensitivity (data not shown).…”

“…Therefore, to confirm the correct sequence and serodiagnostic potential of the dominant epitope of Em2, we synthesized 11 O-glycans with possible structures and examined their antigenicity (Koizumi et al 2009;2011). In the ELISA utilizing these synthetic oligosaccharides as antigens, three biotinylated oligosaccharide probes, Galα1-4Galβ1-3GalNAcα1-R (R 0 biotinylated probe) (H), Galα1-4Galβ1-3(GlcNAcβ1-6)GalNAcα1-R (I), and Galβ1-3(Galα1-4Galβ1-4GlcNAcβ1-6) GalNAcα1-R (K), showed significant serodiagnostic potential in AE diagnosis.…”

Synthesis and Antigenicity against Human Sera of a Biotin-Labeled Oligosaccharide Portion of a Glycosphingolipid from the Parasite <i>Echinococcus multilocularis</i>

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] Our interests have been focused on the unique saccharide chain structure of GSLs and GPs found in several parasites, Echinococcus multilocularis, 5,6,8,12,16) Schistosoma mansoni, 3,7) Ascaris suum 13,14) and Toxocara canis. 4) Among them, we are strongly interested in the structures of GSLs and GPs from E. multilocularis.…”

“…Furthermore, Hülsmeier et al reported 22) that Em2, an antigen extracted from E. multilocularis, is a mucin-type glycoprotein. Based on these information, we synthesized four glycosphingolipids 12) and five carbohydrate structures of glycoproteins 6,8) of E. multilocularis, and examined antigenicity of the pure compounds by ELISA for their serodiagnostic potential. 5,6,23) Among the synthesized compounds (A-I), a glycosphingolipid, Galβ1 6(Fucα1 3) Galβ1 6Galβ1-Cer (D), and a biotinylated carbohydrate, Galα1 4Galβ1 3GalNAcα1-biotin-probe (F), showed good serodiagnostic potential for AE (Fig.…”