Galα1-4Galβ1-3GalNAc is the dominant epitope of Em2 antigen, the mucin-type glycoprotein from Echinococcus multilocularis

Yamano, Kimiaki; Koizumi, Akihiko; Takeda, Tadahiro; Kiuchi, Fumiyuki; Hada, Noriyasu

doi:10.1007/s00436-012-2902-1

Cited by 14 publications

(8 citation statements)

References 37 publications

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“…ELISA was performed as previously described [ 1 , 18 ]. Biotin-labeled oligosaccharides A-E in H 2 O (13 pmol per well) were added to the wells of flat-bottomed microplates (Streptavidin C96, No.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, Antigenicity Against Human Sera and Structure-Activity Relationships of Carbohydrate Moieties from Toxocara larvae and Their Analogues

et al. 2012

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Stereocontrolled syntheses of biotin-labeled oligosaccharide portions containing the Galβ1-3GalNAc core of the TES-glycoprotein antigen obtained from larvae of the parasite Toxocara and their analogues have been accomplished. Trisaccharides Fuc2Meα1-2Gal4Meβ1-3GalNAcα1-OR (A), Fucα1-2Gal4Meβ1-3GalNAcα1-OR (B), Fuc2Meα1-2Galβ1-3GalNAcα1-OR (C), Fucα1-2Galβ1-3GalNAcα1-OR (D) and a disaccharide Fuc2Meα1-2Gal4Meβ1-OR (E) (R = biotinylated probe) were synthesized by block synthesis using 5-(methoxycarbonyl)pentyl-2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl-(1→3)-2-azide-4-O-benzyl-2-deoxy-α-D-galactopyranoside as a common glycosyl acceptor. We examined the antigenicity of these five oligosaccharides by enzyme linked immunosorbent assay (ELISA). Our results demonstrate that the O-methyl groups in these oligosaccharides are important for their antigenicity and the biotinylated oligosaccharides A, B, C and E have high serodiagnostic potential to detect infections caused by Toxocara larvae.

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Section: Methodsmentioning

confidence: 99%

Synthesis, Antigenicity Against Human Sera and Structure-Activity Relationships of Carbohydrate Moieties from Toxocara larvae and Their Analogues

et al. 2012

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“…It makes sense that most of these antibodies are specifically bound to the mucin glycans, that is that the LL is antigenic. When the fragmentary information available is put together, including data for E. multilocularis , the suggestion reached is that a major antigenic carbohydrate motif in E. granulosus infection may be the Galα1‐4Galβ1‐3Gal trisaccharide, which terminates many LL O ‐glycans . Whether in the infection context the LL suffices to generate anticarbohydrate antibody responses or there is a role for priming by oncosphere (and in secondary infection, PSC) glycans is not known.…”

Section: Molecules Making Up the Llmentioning

confidence: 99%

Parasite molecules and host responses in cystic echinococcosis

Díaz

Casaravilla

Barrios

et al. 2016

Parasite Immunology

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Cystic echinococcosis is the infection by the larvae of cestode parasites belonging to the Echinococcus granulosus sensu lato species complex. Local host responses are strikingly subdued in relation to the size and persistence of these larvae, which develop within mammalian organs as 'hydatid cysts' measuring up to tens of cm in diameter. In a context in which helminth-derived immune-suppressive, as well as Th2-inducing, molecules garner much interest, knowledge on the interactions between E. granulosus molecules and the immune system lags behind. Here, we discuss what is known and what are the open questions on E. granulosus molecules and structures interacting with the innate and adaptive immune systems, potentially or in demonstrated form. We attempt a global biological approach on molecules that have been given consideration primarily as protective (Eg95) or diagnostic antigens (antigen B, antigen 5). We integrate glycobiological information, which traverses the discussions on antigen 5, the mucin-based protective laminated layer and immunologically active preparations from protoscoleces. We also highlight some less well-known molecules that appear as promising candidates to possess immune-regulatory activities. Finally, we point out gaps in the molecular-level knowledge of this infectious agent that hinder our understanding of its immunology.

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] Our interests have been focused on the unique saccharide chain structure of GSLs and GPs found in several parasites, Echinococcus multilocularis, 5,6,8,12,16) Schistosoma mansoni, 3,7) Ascaris suum 13,14) and Toxocara canis. 4) Among them, we are strongly interested in the structures of GSLs and GPs from E. multilocularis.…”

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confidence: 99%

“…Based on these information, we synthesized four glycosphingolipids 12) and five carbohydrate structures of glycoproteins 6,8) of E. multilocularis, and examined antigenicity of the pure compounds by ELISA for their serodiagnostic potential. 5,6,23) Among the synthesized compounds (A-I), a glycosphingolipid, Galβ1 6(Fucα1 3) Galβ1 6Galβ1-Cer (D), and a biotinylated carbohydrate, Galα1 4Galβ1 3GalNAcα1-biotin-probe (F), showed good serodiagnostic potential for AE (Fig. 1).…”

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confidence: 99%

Synthesis and Antigenicity against Human Sera of a Biotin-Labeled Oligosaccharide Portion of a Glycosphingolipid from the Parasite <i>Echinococcus multilocularis</i>

Hada

Kitamura

Yamano

et al. 2016

CHEMICAL & PHARMACEUTICAL BULLETIN

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Synthesis of a biotinylated analog of the carbohydrate portion of a glycosphingolipid from the parasite Key words glycosphingolipid; Echinococcus multilocularis; biotin probe; chemical synthesisIn our continuing studies to elucidate the biological function of carbohydrate moieties of glycosphingolipids (GSLs) and glycoproteins (GPs) from invertebrates, we have synthesized oligosaccharides found in various Protostomia phyla.

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Galα1-4Galβ1-3GalNAc is the dominant epitope of Em2 antigen, the mucin-type glycoprotein from Echinococcus multilocularis

Cited by 14 publications

References 37 publications

Synthesis, Antigenicity Against Human Sera and Structure-Activity Relationships of Carbohydrate Moieties from Toxocara larvae and Their Analogues

Synthesis, Antigenicity Against Human Sera and Structure-Activity Relationships of Carbohydrate Moieties from Toxocara larvae and Their Analogues

Parasite molecules and host responses in cystic echinococcosis

Synthesis and Antigenicity against Human Sera of a Biotin-Labeled Oligosaccharide Portion of a Glycosphingolipid from the Parasite <i>Echinococcus multilocularis</i>

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