Synthetic Sequence-specific Ligands

Gursky, G. V.; Заседателев, А. С.; Zhuze, A. L.; Khorlin, A.A.; Grokhovsky, S. L.; Streltsov, S. А.; Surovaya, A. N.; Nikitin, S.; Крылов, А. С.; Retchinsky, V.O.; Mikhailov, Michael; Beabealashvili, R.Sh.; Gottikh, B. P.

doi:10.1101/sqb.1983.047.01.043

Cited by 78 publications

(49 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1) (.sios = 40,000) was synthesized and purified as described earlier [4]. Poly(dA)' poly(dT) (&Z60 = 12,000) and poWWV1 pWWV1 @m = 13,600) were obtained from P.L.…”

Section: Methodsmentioning

confidence: 99%

Mono‐, di‐ and trimeric binding of a bis‐netropsin to DNA

et al. 1995

Self Cite

View full text Add to dashboard Cite

“…1) (.sios = 40,000) was synthesized and purified as described earlier [4]. Poly(dA)' poly(dT) (&Z60 = 12,000) and poWWV1 pWWV1 @m = 13,600) were obtained from P.L.…”

Section: Methodsmentioning

confidence: 99%

Mono‐, di‐ and trimeric binding of a bis‐netropsin to DNA

et al. 1995

Self Cite

View full text Add to dashboard Cite

“…Since each of Nt subunits may separately bind DNA, the bis-Nts are capable of bidendate interaction with DNA [13,14,20]. It is reasonable to assume that the ligands which arc able to bind specifically to longer stretches of DNA in the minor groove should be more potent topo I inhibitors.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous studies we synthesized a seria of high sequence-specific derivatives ofbis-Nts [11,12] and analyzed their ability to bind DNA and to inhibit a number of DNA-directed enzymes [13][14][15]. The choice of these bis-Nt derivatives ( The present paper describes the effects of this new seria ofbis-Nts capable of bidendate binding to DNA (Fig.…”

Section: Introductionmentioning

confidence: 99%

Human DNA‐topoisomerase I activity is affected by bis‐netropsin's binding to DNA minor groove

et al. 1998

View full text Add to dashboard Cite

SUMMARYBis-netropsins (bis-Nts) are known to be efficient inhibitors of human DNA topoisomerase (topo) I with a higher antitumor activity than netropsin. New sequence-specific derivatives of bis-Nts were used for modulation of topo I-mediated DNA cleavage with and without camptothecin (CPT). Relation between the bis-Nts binding sites and topo I cleavage sites has been analyzed with the plasmid DNA constructs generated by insertion of synthetic oligonucleotides containing various topo I-cleavage and bis-Nt-binding sites. These constructs offer an opportunity to study minor groove binders effects on the topo I reaction on DNA. Three major effects: (i) bis-Nt -mediated disappearance of some of the topo I cleavage sites; (ii) enhancement of some other sites, and, (iii) generation of new cleavage sites, have been found and analyzed. These effects demonstrate that bis-Nts drastically change the topo Imediated DNA cleavage.

show abstract

“…In addition, structural changes arise in DNA when the parameters of its aqueous environment are changed or various ligands are bound. A convenient model for studying the local parameters of the double helix is provided by low-molecular-weight sequence-specific ligands, which bind to particular DNA sequences (Gursky et al, 1983;Zimmer, Ch. & Wahnert, 1986;Bailly et al, 2005).…”

Section: Ultrasonic Cleavage Of Nicked Dnamentioning

confidence: 99%