2017
DOI: 10.1007/978-1-4939-6940-1_1
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Synthetic Protein Switches: Theoretical and Experimental Considerations

Abstract: Synthetic protein switches with tailored response functions are finding increasing applications as tools in basic research and biotechnology. With a number of successful design strategies emerging, the construction of synthetic protein switches still frequently necessitates an integrated approach that combines detailed biochemical and biophysical characterization in combination with high-throughput screening to construct tailored synthetic protein switches. This is increasingly complemented by computational st… Show more

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Cited by 5 publications
(1 citation statement)
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“…Inspiration can be taken from nature, where signaling is underpinned by protein switches that sense and respond to molecular inputs . Engineered protein switches have been designed for synthetic biology and sensing applications and could be transformational as a rapid alternative to laboratory immunoassays. Immunoassays typically utilize separate reagents for analyte recognition and signal generation, necessitating multiple immobilization and wash steps that prohibit direct point-of-need use (Figure A) . Microfluidic lab-on-a-chip systems can automate the steps, but few, like the Abbott iSTAT point-of-care analyzer, have been commercially successful .…”
mentioning
confidence: 99%
“…Inspiration can be taken from nature, where signaling is underpinned by protein switches that sense and respond to molecular inputs . Engineered protein switches have been designed for synthetic biology and sensing applications and could be transformational as a rapid alternative to laboratory immunoassays. Immunoassays typically utilize separate reagents for analyte recognition and signal generation, necessitating multiple immobilization and wash steps that prohibit direct point-of-need use (Figure A) . Microfluidic lab-on-a-chip systems can automate the steps, but few, like the Abbott iSTAT point-of-care analyzer, have been commercially successful .…”
mentioning
confidence: 99%