2021
DOI: 10.1016/bs.mie.2020.09.009
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Synthetic protein switches: Combinatorial linker engineering with iFLinkC

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Cited by 4 publications
(8 citation statements)
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“…In the context of the iFLinkC-X assembly framework, α- and ω-plasmids strictly feature Gly at their N- and C-terminal ends (Figure D). In this way, the resultant linker, once assembled, is readily compatible with the parent iFLinkC assembly process , (Figure A). Apart from that, the α- and ω-plasmids generally code for the two lowest indices of a linker motif, while the bridging amino acid X on the inside can be flexibly chosen to connect arbitrary linker fragments depending on the predominant amino acid in a polymeric linker repeat (Figure D).…”
Section: Resultsmentioning
confidence: 99%
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“…In the context of the iFLinkC-X assembly framework, α- and ω-plasmids strictly feature Gly at their N- and C-terminal ends (Figure D). In this way, the resultant linker, once assembled, is readily compatible with the parent iFLinkC assembly process , (Figure A). Apart from that, the α- and ω-plasmids generally code for the two lowest indices of a linker motif, while the bridging amino acid X on the inside can be flexibly chosen to connect arbitrary linker fragments depending on the predominant amino acid in a polymeric linker repeat (Figure D).…”
Section: Resultsmentioning
confidence: 99%
“…We recently developed a DNA assembly process termed iterative functional linker cloning (iFLinkC) which enables the fusion of any two protein coding sequences, for example, a functional domain with a linker element, via a single Gly residue , (Figure A). Assembly is achieved through the combined action of several type IIS restriction enzymes and T4 DNA ligase.…”
Section: Resultsmentioning
confidence: 99%
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“…Engineering Recombinant Nanopore Assemblies. Finally, a set of recombinant nanopores was engineered by recombining the truncated S 21 56 variant with ring-shaped oligomeric protein structures and linkers of varying length and flexibility 46,47 (Figure 6A). Ring-shaped oligomeric protein structures were drawn from members of the Lsm protein family AfSm2, 48 AfSm1, 49 and ScLsm3 50 featuring defined hexa-, hepta-, and octameric geometries.…”
Section: ■ Resultsmentioning
confidence: 99%