Synthetic poly(vinyl alcohol)–chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility

Zhao, Yifan; Zhao, Jingyu; Hu, Wenzhi; Ma, Kui; Yin, Chao; Sun, Pengchao; Fu, Xiaobing; Zhang, Hong

doi:10.1039/c8tb03181a

Cited by 89 publications

(48 citation statements)

References 38 publications

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“…Despite the porous and three-dimensional structure of sponges promoting blood clotting, soft and injectable materials such as hemostatic hydrogels have attracted research interest within the past decade. Previous studies have reported that the hemostatic hydrogels possess the unique ability to adequately fill and adhere to irregular wounds, contributing to more efficient hemorrhage control [ 11 , 12 , 13 ]. Moreover, soft hemostatic hydrogels could be easily removed through water or phosphate buffered saline (PBS), which can be carried out to reduce secondary wound bleeding [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

An Injectable Hybrid Gelatin Methacryloyl (GelMA)/Phenyl Isothiocyanate-Modified Gelatin (Gel-Phe) Bioadhesive for Oral/Dental Hemostasis Applications

et al. 2021

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Biomaterials are widely used for effectively controlling bleeding in oral/dental surgical procedures. Here, gelatin methacryloyl (GelMA) was synthesized by grafting methacrylic anhydride on gelatin backbone, and phenyl isothiocyanate-modified gelatin (Gel-Phe) was synthesized by conjugating different gelatin/phenyl isothiocyanate molar ratios (G/P ratios) (i.e., 1:1, 1:5, 1:10, 1:15, 1:25, 1:50, 1:100, and 1:150) with gelatin polymer chains. Afterward, we combined GelMA and Gel-Phe as an injectable and photo-crosslinkable bioadhesive. This hybrid material system combines photo-crosslinking chemistry and supramolecular interactions for the design of bioadhesives exhibiting a highly porous structure, injectability, and regulable mechanical properties. By simply regulating the G/P ratio (1:1–1:15) and UV exposure times (15–60 s), it was possible to modulate the injectability and mechanical properties of the GelMA/Gel-Phe bioadhesive. Moreover, we demonstrated that the GelMA/Gel-Phe bioadhesive showed low cytotoxicity, a highly porous network, and the phenyl-isothiourea and amine residues on Gel-Phe and GelMA polymers with synergized hemostatic properties towards fast blood absorption and rapid clotting effect. An in vitro porcine skin bleeding and an in vitro dental bleeding model confirmed that the bioadhesive could be directly extruded into the bleeding site, rapidly photo-crosslinked, and reduced blood clotting time by 45%. Moreover, the in situ crosslinked bioadhesive could be easily removed from the bleeding site after clotting, avoiding secondary wound injury. Overall, this injectable GelMA/Gel-Phe bioadhesive stands as a promising hemostatic material in oral/dental surgical procedures.

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Section: Introductionmentioning

confidence: 99%

An Injectable Hybrid Gelatin Methacryloyl (GelMA)/Phenyl Isothiocyanate-Modified Gelatin (Gel-Phe) Bioadhesive for Oral/Dental Hemostasis Applications

et al. 2021

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“…Genipin/PVA/chitosan buccal patch (Formulation 4) and a commercial chitosan‐based dressing were placed into glass tubes at 37°C and the mixture of blood and citrate dextrose was placed on the samples. The resulting samples were examined by inverting the tubes until the blood flow completely stopped and the clotting time was recorded 45 …”

Section: Methodsmentioning

confidence: 99%

Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications

Shirvan

Hemmatinejad

Bahrami

et al. 2021

J Biomedical Materials Res

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Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders. The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications. The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer‐by‐layer process. An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss. Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties. The swelling index of the patches was examined with respect to the PVA/chitosan ratio. The effect of genipin addition to the electrospinning solution was also studied on mucoadhesive and swelling properties. The cell viability of buccal patches was assessed by methylthiazolydiphenyl‐tetrazolium bromide test on L929 fibroblast cell line. The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria. The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.

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“…The model of skin defect: [21,22] the anesthetized SD rats were fixed on the operating table, after the hair of their back was shaved, a full-thickness defect with a diameter of 10 mm was built on the back. The observed animals were randomly divided into the hydrogel experimental group and the blank control group (n = 5).…”

Section: Animal Experimental Studymentioning

confidence: 99%

By Endowing Polyglutamic Acid/Polylysine Composite Hydrogel with Super Intrinsic Characteristics to Enhance its Wound Repair Potential

Wei

Chen

Wang

et al. 2021

Macromolecular Bioscience

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Among these biomaterials, hydrogels have attracted much attention. The reason is that hydrogels are easier to graft and modify than other materials, and are more versatile. Moreover, their unique extracellular matrix structure and high moisture retention are beneficial for forming a good wound healing environment. [8,9] However, most of the traditional hydrogels used for wound repair have poor adhesion, hemostasis and antibacterial function, thus limiting their clinical application. [10][11][12] This study would focus on the above problems, and develop a multifunctional PGA-PLL-Ca composite hydrogel with good adhesion, moisture retention and hemostatic effect, and excellent antibacterial properties that can promote wound healing.In this work, such multifunctional PGA-PLL-Ca composite hydrogel would be synthesized using poly glutamic acid and polylysine as precursors, casein as foaming agent and calcium as coagulant. Hydrophilic group and positively charged amino group in the PGA-PLL-Ca composite hydrogel would create excellent hydrophilicity, moisture retention, adhesion and antibacterial properties for hydrogels, while casein would endow hydrogel with uniform porous structure, and the introduction of calcium ions would provide with good hemostatic properties. These unique properties could not only facilitate hemostasis, but also provide a moist, sterile and closed microenvironment for wound to heal better. Up to date, this kind of multifunctional material design is rarely reported. Experimental Section Materialsγ-Polyglutamic acid (M W ≈ 800 kDa), ε-polylysine (M W ≈ 3000), casein (chemical grade), anhydrous calcium chloride (≥96%), 1-hydroxypyrrolidine (NHS, ≥98%), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC⋅HCl, ≥98.5%) were purchased from Xi'an Bella Biotech-

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Synthetic poly(vinyl alcohol)–chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility

Abstract: Synthetic poly(vinyl alcohol)–chitosan as a new type of highly efficient hemostatic sponge with blood-triggered swelling and high biocompatibility.

Cited by 89 publications

References 38 publications

An Injectable Hybrid Gelatin Methacryloyl (GelMA)/Phenyl Isothiocyanate-Modified Gelatin (Gel-Phe) Bioadhesive for Oral/Dental Hemostasis Applications

An Injectable Hybrid Gelatin Methacryloyl (GelMA)/Phenyl Isothiocyanate-Modified Gelatin (Gel-Phe) Bioadhesive for Oral/Dental Hemostasis Applications

Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications

By Endowing Polyglutamic Acid/Polylysine Composite Hydrogel with Super Intrinsic Characteristics to Enhance its Wound Repair Potential

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