Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage

Yim, Evelyn K.F.; Pang, S. W.; Leong, Kam W.

doi:10.1016/j.yexcr.2007.02.031

Cited by 702 publications

(725 citation statements)

References 34 publications

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“…On the titanium nanotubes, tube diameters of 70 and 100 nm enhanced the expression of different osteogenic markers in mesenchymal stem cells compared with cells on a flat titanium surface [21]. On nanogratings, also an up-regulation of neuronal markers, such as microtubule-associated protein 2, was observed in mesenchymal stem cells [22]. This effect of the nanostructure on the neuronal differentiation was stronger than biochemical cues which were added to induce neuronal differentiation.…”

Section: Cell Regulation By Physical Characteristics Of a Materials Tomentioning

confidence: 92%

Cell-material interaction

Rychly

Nebe

2013

BioNanoMaterials

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Abstract:The interaction of cells with material surfaces is of fundamental relevance and contributes to the clinical success of implants. Cells sense their physico-chemical environment resulting in focal adhesion reorganization, spatio-temporal localization and activation of integrin dependent signalling proteins as well as phenotypic changes, e.g., of the actin cytoskeleton. The technology of designing instructive biomaterials involves chemical modifications by grafting of chemical groups, adhesion ligands and growth factors. Physical modifications of the materials are created by structuring the surfaces stochastically and geometrically and by modifications of the material stiffness. Insights into the mechanism at the interface that are involved in the regulation of cells such as stem cells, osteoblasts and chondrocytes by materials will advance the development of innovative biomaterials in regenerative medicine.

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Section: Cell Regulation By Physical Characteristics Of a Materials Tomentioning

confidence: 92%

Cell-material interaction

Rychly

Nebe

2013

BioNanoMaterials

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“…order/disorder. In a different study, Yim et al [83] demonstrated that nanograted substrata were able to induce transdifferentiation of human mesenchymal stem cells (MSCs) in neurons.…”

Section: Topo-cue-mediated Crosstalkmentioning

confidence: 99%

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Ventre

Causa

Netti

2012

J. R. Soc. Interface.

158

153

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The development of novel biomaterials able to control cell activities and direct their fate is warranted for engineering functional biological tissues, advanced cell culture systems, single-cell diagnosis as well as for cell sorting and differentiation. It is well established that crosstalk at the cell–material interface occurs and this has a profound influence on cell behaviour. However, the complete deciphering of the cell–material communication code is still far away. A variety of material surface properties have been reported to affect the strength and the nature of the cell–material interactions, including biological cues, topography and mechanical properties. Novel experimental evidence bears out the hypothesis that these three different signals participate in the same material–cytoskeleton crosstalk pathway via adhesion plaque formation dynamics. In this review, we present the relevant findings on material-induced cell response along with the description of cell behaviour when exposed to arrays of signals—biochemical, topographical and mechanical. Finally, with the aid of literature data, we attempt to draw unifying elements of the material–cytoskeleton–cell fate chain.

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“…It is therefore reasonable to expect that the presentation of topographic signals is one of the strategies that Nature adopts to impart to cells specific orders, which eventually dictate their behaviour. In fact, many works demonstrated that patterned substrates strongly influence cell adhesion, migration and differentiation, suggesting that the presentation of topographic signals might be a powerful tool to control and guide cell behaviour in vitro [4][5][6][7][8]. Indeed, recent literature has addressed the importance of the material-cytoskeleton crosstalk, which is at the helm of the biophysical and biochemical stimuli eventually governing cell fate and functions [9].…”

Section: Introductionmentioning

confidence: 99%

Topographic cell instructive patterns to control cell adhesion, polarization and migration

Ventre

Natale

Rianna

et al. 2014

J. R. Soc. Interface.

105

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Topographic patterns are known to affect cellular processes such as adhesion, migration and differentiation. However, the optimal way to deliver topographic signals to provide cells with precise instructions has not been defined yet. In this work, we hypothesize that topographic patterns may be able to control the sensing and adhesion machinery of cells when their interval features are tuned on the characteristic lengths of filopodial probing and focal adhesions (FAs). Features separated by distance beyond the length of filopodia cannot be readily perceived; therefore, the formation of new adhesions is discouraged. If, however, topographic features are separated by a distance within the reach of filopodia extension, cells can establish contact between adjacent topographic islands. In the latter case, cell adhesion and polarization rely upon the growth of FAs occurring on a specific length scale that depends on the chemical properties of the surface. Topographic patterns and chemical properties may interfere with the growth of FAs, thus making adhesions unstable. To test this hypothesis, we fabricated different micropatterned surfaces displaying feature dimensions and adhesive properties able to interfere with the filopodial sensing and the adhesion maturation, selectively. Our data demonstrate that it is possible to exert a potent control on cell adhesion, elongation and migration by tuning topographic features' dimensions and surface chemistry.

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Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage

Cited by 702 publications

References 34 publications

Cell-material interaction

Cell-material interaction

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Topographic cell instructive patterns to control cell adhesion, polarization and migration

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