Synthetic bone scaffolds and fracture repair

Carson, Joshua; Bostrom, Mathias P.

doi:10.1016/j.injury.2007.02.008

Cited by 141 publications

(112 citation statements)

References 18 publications

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“…However, in clinic osseointegration is often compromised by large peri-implant bone defect. Several regional bone regeneration techniques including bone tissue engineering has been reported to solve such peri-implant bone defect [1][2][3] . Bone morphogenetic proteins (BMPs) emerge as promising candidates for bone tissue engineering due to their significant effects on bone and cartilage growth 4,5) .…”

Section: Introductionmentioning

confidence: 99%

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer

Sun

Wang

Zheng

et al. 2012

Dental Materials Journal

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This study aimed to compare the effects of bone morphogenetic protein BMP2/7 heterodimer and BMP homodimers on bone regeneration in bone defects model. Identical peri-implant bone defects model were created using proper controls on the frontal skull in 18 minipigs. Collagen sponges with low-dose (30 ng/mL) BMP2/7 heterodimer, BMP2 or BMP7 homodimer were filled in the defects. New bone formation and the expression of type I collagen (Col1), alkaline phosphatase (ALP) and osteocalcin (OCN) were evaluated after 2, 3, and 6 weeks of implantation. BMP2/7 resulted in significantly higher new bone areas percentage in the defect region than BMP2 and BMP7 (p<0.05). Immunohistochemical staining of Col1, ALP and OCN was stronger in BMP2/7 group than BMP2, BMP7 and control group (p<0.05). These results demonstrate that BMP2/7 heterodimer is a stronger inducer of osteoblastogenesis and could be applied at low dose to reduce the cost and side effects of BMP homodimers.

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Section: Introductionmentioning

confidence: 99%

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer

Sun

Wang

Zheng

et al. 2012

Dental Materials Journal

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show abstract

“…Furthermore, to resolve the problems of nonbioabsorbable materials, recently, biodegradable materials, such as poly-l-lactic acid (PLLA) and polylactic-glycolic-acid (PLGA), have been used. Those materials can be fabricated for a wide range of biomedical applications with a high processability, controlled degradation, adjustable mechanical properties, and the possibility for modification [4][5][6] . In particular, PLGA has been used extensively in the biomaterial field and is generally considered to be biocompatible.…”

Section: Introductionmentioning

confidence: 99%

Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium

et al. 2017

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In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells.

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“…1,2 One rational challenge in this area is to create scaffolds that could provide an appropriate extracellular matrix (ECM) for efficient adhesion, survey, proliferation, and differentiation of therapeutic cellular products that will be further applied to an injured tissue. However, perfect mimicry is difficult to achieve, considering the complex molecular, cellular, and mechanical processes requiring being timely coordinated for tissue repair.…”

Section: Introductionmentioning

confidence: 99%

Glycosaminoglycan Mimetic Associated to Human Mesenchymal Stem Cell-Based Scaffolds Inhibit Ectopic Bone Formation, but Induce Angiogenesis In Vivo

Frescaline

Bouderlique

Mansoor

et al. 2013

Tissue Engineering Part A

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Tissue engineering approaches to stimulate bone formation currently combine bioactive scaffolds with osteocompetent human mesenchymal stem cells (hMSC). Moreover, osteogenic and angiogenic factors are required to promote differentiation and survival of hMSC through improved vascularization through the damaged extracellular matrix (ECM). Glycosaminoglycans (GAGs) are ECM compounds acting as modulators of heparinbinding protein activities during bone development and regenerative processes. GAG mimetics have been proposed as ECM stabilizers and were previously described for their positive effects on bone formation and angiogenesis after local treatment. Here, we developed a strategy associating the GAG mimetic [OTR 4120 ] with bone substitutes to optimize stem cell-based therapeutic products. We showed that [OTR 4120 ] was able to potentiate proliferation, migration, and osteogenic differentiation of hMSC in vitro. Its link to tricalcium phosphate/hydroxyapatite scaffolds improved their colonization by hMSC. Surprisingly, when these combinations were tested in an ectopic model of bone formation in immunodeficient mice, the GAG mimetics inhibit bone formation induced by hMSC and promoted an osteoclastic activity. Moreover, the inflammatory response was modulated, and the peri-implant vascularization stimulated. All together, these findings further support the ability of GAG mimetics to organize the local ECM to coordinate the host response toward the implanted biomaterial, and to inhibit the abnormal bone formation process on a subcutaneous ectopic site.

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Synthetic bone scaffolds and fracture repair

Cited by 141 publications

References 18 publications

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer

Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium

Glycosaminoglycan Mimetic Associated to Human Mesenchymal Stem Cell-Based Scaffolds Inhibit Ectopic Bone Formation, but Induce Angiogenesis In Vivo

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