KuTZNE R , AND J. CHR ISTOPHE. Amirio acid Icv els in rat paticreos after pilocarpine or pancreozymin. Am. J. Physiol. 224(6): 1309-1313. 1973.-The levels of a séries of nonessential amino acids were rapidly altered in the rat exocrine pancréas after administration of secretogogues. The peak secretory response observed at 30 min after the intraperitoneal injection of pilocarpine (30 mg/kg) was accompanied by élévations of alanine (+ 238%) and aspartate (+ 66%) and diminutions of glutamine (-31%) and glutamate (-20%). Thèse changes suggest that the neural stimulation of pancreatic sécrétion was coupled with changes in redox potentials and oxidative phosphorylation. The shorter secretory cycle in duced by pancreozymin (23 Crick, Harper, and Râper U/kg administered intravenously under Nembutal anesthesia) also led to opposite variations of aspartate, glutamine, and glutamate. Aspartate almost tripled in the pancréas after 10 min, whereas glutamine and glutamate levels were depressed by 43 and 31%, respectively. In the liver, pilocarpine stimulation increased the levels of alanine (+ 71%) and aspartate (+ 43%) and decreased glutamine (-49%) at 30 min postinjection. Pancreozymin ad ministration was not associated with rapid changes in levels of liver amino acids. exocrine pancréas; sécrétion; amino acid metabolism ALTERATION S IN PANCREATIC amino acid levels in response to secretogogues bave not as yet been documented. The discharge of secretory proteins into the lumen in volves propulsion of zymogen granules to the apex of acinar cells, membrane fusion fission, and reutilization of mem brane components (1, 16). Thèse processes are dépendent on respiratory energy (3, 9, 15). Their metabolic needs ap pear to rely inostly on endogenous fatty acids (14) and to a lesser extent on glucose oxidation (3). The participation of free amino acids is uncertain. Furthermore, contradictory results have been published on the concurrent cfTects of secretogogues on pancreatic protein svnthesis (3, 10, 16, 17, 20, 23, 24, 2629). The présent investigation vsas undertaken to ascertain whether amino acids levels were altered so rapidly as to suspect a metabolic relationship between amino acid metabolism and sécrétion. This problem was explored by using the natural hormone pancreozymincholecystokinin, and also with pilocarpine. Pilocarpine is an alkaloid exert ing a potent parasympathomimetic eP'ect on muscarinic receptors and is known to synchronize a secretory cycle in the pancréas (21).