Effects of Fasting, Feeding, and Bethanechol Chloride on Pancreatic Microsomal Protein Synthesis in Vitro

Morisset, J.; Black, Owen; Webster, Peter

doi:10.3181/00379727-140-36664

Cited by 11 publications

(2 citation statements)

References 3 publications

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“…It is known that the phosphorylation of S6 can be regulated by CCK (4,5,36), insulin (42), and the branched-chain amino acid leucine (37) in rat pancreas as has been shown in other organs (2,23,49). Cholinergic stimulation and insulin have been demonstrated to activate protein synthesis in pancreas (21,28,48) and the translational machinery in other organs (15,32,48) as well. Thus the stimuli involved in the food intake process that increases vagal neural activity, plasma CCK levels and protein and aminoacids from the food itself (41), could each contribute to the stimulation of pancreatic protein synthesis (14,22) and the activation of translational machinery in the exocrine pancreas after a meal.…”

Section: Discussionmentioning

confidence: 87%

Feeding activates protein synthesis in mouse pancreas at the translational level without increase in mRNA

Sans

Lee

DʼAlecy

et al. 2004

American Journal of Physiology-Gastrointestinal and Liver Physi

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To determine the mechanism of meal-regulated synthesis of pancreatic digestive enzymes, we studied the effect of fasting and refeeding on pancreatic protein synthesis, relative mRNA levels of digestive enzymes, and activation of the translational machinery. With the use of the flooding dose technique with L-[3H]phenylalanine, morning protein synthesis in the pancreas of Institute for Cancer Research mice fed ad libitum was 7.9 +/- 0.3 nmol phenylalanine.10 min(-1).mg protein(-1). Prior fasting for 18 h reduced total protein synthesis to 70 +/- 1.4% of this value. Refeeding for 2 h, during which the mice consumed 29% of their daily food intake, increased protein synthesis to 117.3 +/- 4.9% of the control level. Pancreatic mRNA levels of amylase, lipases, trypsins, chymotrypsin, elastases, as well as those for several housekeeping genes tested were not significantly changed after refeeding compared with fasted mice. By contrast, the major translational control pathway involving Akt, mTOR, and S6K was strongly regulated by fasting and refeeding. Fasting for 18 h decreased phosphorylation of ribosomal protein S6 to almost undetectable levels, and refeeding highly increased it. The most highly phosphorylated form of the eIF4E binding protein (4E-BP1) made up the 14.6% of total 4E-BP1 in normally fed animals, was only 2.8% after fasting, and was increased to 21.4% after refeeding. This was correlated with an increase in the formation of the eIF4E-eIF4G complex after refeeding. By contrast, feeding did not affect eIF2B activity. Thus food intake stimulates pancreatic protein synthesis and translational effectors without increasing digestive enzyme mRNA levels.

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Section: Discussionmentioning

confidence: 87%

Feeding activates protein synthesis in mouse pancreas at the translational level without increase in mRNA

Sans

Lee

DʼAlecy

et al. 2004

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…It is therefore particularly vulnerable to disease states, and the effects of malnutrition may be profound with fibrosis, acinar atrophy and marked reduction in secretion [29,72,78]. Pancreatic endocrine function, however, does appear to be relatively spared.…”

Section: Effects Of Undernutrition On Pancreatic Functionmentioning

confidence: 93%

The effects of undernutrition and refeeding on metabolism and digestive function

Winter

2006

Current Opinion in Clinical Nutrition and Metabolic Care

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Degeneration of mouse pancreatic acinar cells during fasting

Nevalainen

Janigan

1974

Virchows Arch. Abt. B Zellpath.

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Effects of Fasting, Feeding, and Bethanechol Chloride on Pancreatic Microsomal Protein Synthesis in Vitro

Cited by 11 publications

References 3 publications

Feeding activates protein synthesis in mouse pancreas at the translational level without increase in mRNA

Feeding activates protein synthesis in mouse pancreas at the translational level without increase in mRNA

The effects of undernutrition and refeeding on metabolism and digestive function

Degeneration of mouse pancreatic acinar cells during fasting

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