Synthesized zinc peroxide nanoparticles (ZnO₂-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Abstract

Increasing of multidrug resistance (MDR) remains an intractable challenge for burn patients. Innovative nanomaterials are also in high demand for the development of new antimicrobial biomaterials that inevitably have opened new therapeutic horizons in medical approaches and lead to many efforts for synthesizing new metal oxide nanoparticles (NPs) for better control of the MDR associated with the polymicrobial burn wounds. Recently, it seems that metal oxides can truly be considered as highly efficient inorganic agents with antimicrobial properties. In this study, zinc peroxide NPs (ZnO 2 -NPs) were synthesized using the co-precipitation method. Synthesized ZnO 2 -NPs were characterized by X-ray diffraction, Fourier transformed infrared, transmission electron microscopy, thermogravimetric analysis, differential scanning calorimetry, and ultraviolet-visible spectroscopy. The characterization techniques revealed synthesis of the pure phase of non-agglomerated ZnO 2 -NPs having sizes in the range of 15–25 nm with a transition temperature of 211°C. Antimicrobial activity of ZnO 2 -NPs was determined against MDR Pseudomonas aeruginosa (PA) and Aspergillus niger (AN) strains isolated from burn wound infections. Both strains, PA6 and AN4, were found to be more susceptible strains to ZnO 2 -NPs. In addition, a significant decrease in elastase and keratinase activities was recorded with increased concentrations of ZnO 2 -NPs until 200 µg/mL. ZnO 2 -NPs revealed a significant anti-inflammatory activity against PA6 and AN4 strains as demonstrated by membrane stabilization, albumin denaturation, and proteinase inhibition. Moreover, the results of in vivo histopathology assessment confirmed the potential role of ZnO 2 -NPs in the improvement of skin wound healing in the experimental animal models. Clearly, the synthesized ZnO 2 -NPs have demonstrated a competitive capability as antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory candidates, suggesting that the ZnO 2 -NPs are promising metal oxides that are potentially valued for biomedical applications.

Nanoplatforms of Manganese Ferrite Nanoparticles Functionalized with Anti‐Inflammatory Drugs

“…Acetylsalicylic acid has shown a dose‐dependent ability to inhibit the induced protein denaturation . Zinc peroxide, silver and iron nanoparticles have also been tested as anti‐inflammatory agents via inhibition of denaturation and have been found of concentration dependent anti‐inflammatory activity , , . Herein, we aimed at examining both the anti‐inflammatory capacity of MnFe 2 O 4 MNPs and the collective properties of MNPs coupled and/or loaded with ASA.…”

Rapid Immobilization of Cellulase onto Graphene Oxide with a Hydrophobic Spacer

“…It is well known that GO contained epoxy, carbonyl, and hydroxyl groups [27] and the modification is easier than other nanoparticles [28][29][30][31][32][33]. In our previous work, GO derivatives were used as the carrier for immobilization of cellulose [2] and NADH oxidase [22], and the immobilized enzyme retain higher stability and good reusability.…”

Phytochemical, Gas Chromatography With Mass Spectrometry Analysis of Andrographis Serpyllifolia Methanol Leaf Extract and Its Antioxidant and Antibacterial Activities

“…Burn followed by microbial infection is a very serious complication that often results in the patients' death [13]. About 45% of mortality is recorded in burned patients as a consequence of microbial infections [14]. On the other hand, the WHO regarding drug resistance has encouraged and promoted screening and utilization of medicinal plants as a new alternative therapy against multi-drug resistant pathogens that cause severe infections and difficult-to-treat diseases [15].…”