Synthesis, radiolabeling, and in vivo evaluation of an 18F-labeled isatin analog for imaging caspase-3 activation in apoptosis

Chu, Wenhua; Rothfuss, Justin; Zeng, Chenbo; Xu, Jinbin; Jones, Lynne A.; Welch, Michael J.; Mach, Robert H.

doi:10.1016/j.bmcl.2006.07.045

Cited by 101 publications

(89 citation statements)

References 14 publications

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“…Caspases are also implicated in apoptosis, and caspase-3 has recently been labeled with 18 F (77). In a murine model of hepatic apoptosis, highly specific liver uptake of this tracer was shown (78). Further experiments determining the usefulness in cardiovascular applications of 18 F-labeled caspase-3 tracers seem justified.…”

Section: Evaluation Of Known or Suspected Cardiomyopathymentioning

confidence: 99%

Hybrid PET/MR Imaging of the Heart: Potential, Initial Experiences, and Future Prospects

et al. 2013

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Learning Objectives: On successful completion of this activity, participants should be able to describe (1) advantages and shortcomings of hybrid PET/MR scanners with respect to cardiovascular applications (e.g. myocardial perfusion imaging and viability imaging), (2) additional value of the MR component in cardiac imaging, and (3) technical challenges and workflow considerations regarding hybrid PET/MR scanners in the field of cardiology (e.g. attenuation correction and cardiac/respiratory/patient motion).Financial Disclosure: Dr. Schwaiger is an investigator, meeting participant, and lecturer for Siemens Medical. The authors of this article have indicated no other relevant relationships that could be perceived as a real or apparent conflict of interest. CME Credit: SNMMI is accredited by the Accreditation Council for Continuing Medical Education (ACCME) to sponsor continuing education for physicians. SNMMI designates each JNM continuing education article for a maximum of 2.0 AMA PRA Category 1 Credits. Physicians should claim only credit commensurate with the extent of their participation in the activity. For CE credit, participants can access this activity through the SNMMI Web site (http:// www.snmmi.org/ce_online) through March 2016.PET/CT and other combined scanners have in the past decade rapidly emerged as important research tools and are proving to be invaluable for improved diagnostics in routine nuclear medicine. The design of hybrid PET/MR scanners presented a formidable technical challenge, and only recently were these instruments introduced to the market. Initial expectations of the performance of these scanners have been high, notably because of the potential for superior tissue contrast inherent in the MR modality, as well as the potential for multiparametric functional imaging in conjunction with PET. However, the additional value and potential clinical role that these new systems might bring to the cardiac field have yet to be documented. This review presents a comparative summary of the existing applications for PET and MR in the field of cardiology and suggests potential cardiac applications exploiting unique properties of the newly introduced combined instrumentation. The diverse range of imaging modalities for cardiology includes echocardiography, CT, MR imaging, SPECT, and PET, each of which offers distinct properties and advantages. The demand for combined PET/CT instrumentation in clinical nuclear medicine has grown remarkably, because of the notable advantages presented by hybrid imaging with respect to anatomic localization of lesions. Although these advantages were initially driven by the demands of oncology imaging, the resultant broader availability of PET/CT has provided the opportunity to use these scanners for indications beyond oncology, such as in the field of cardiac imaging (1). Furthermore, CT images are necessary for rapid attenuation correction, which is of particular importance for quantification in myocardial perfusion imaging. Through the use of suitable CT components a...

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Section: Evaluation Of Known or Suspected Cardiomyopathymentioning

confidence: 99%

Hybrid PET/MR Imaging of the Heart: Potential, Initial Experiences, and Future Prospects

et al. 2013

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“…Imaging the activity of the caspase cascade could be quite informative as most forms of apoptosis converge on the terminal execution enzyme, caspase-3. 77,78 The backbone of these tracers is based on the 5-pyrrolidinylsulfonyl isatin class of nonpeptidyl caspase inhibitors. The dicarbonyl function of isatins covalently binds to the cysteine residue of the active site of a given caspase.…”

Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

In vivo imaging of apoptosis

Blankenberg¹

2008

Cancer Biology & Therapy

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“…4,8 Imaging the activation of the caspase cascade such as caspase 3 activity has been demonstrated in animals using positron emission tomography (PET). 9,10 However, no human data have ever been published and caspase 3 activation is not necessarily unique to apoptosis. 11,12 On the other hand, the PS externalization is thought to be an early event in apoptosis that closely follows caspase 3 activation.…”

Section: Introductionmentioning

confidence: 99%

Increase of reduced nicotinamide adenine dinucleotide fluorescence lifetime precedes mitochondrial dysfunction in staurosporine-induced apoptosis of HeLa cells

Yu¹,

Guo²,

Wang

et al. 2011

J. Biomed. Opt.

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Abstract.In vivo noninvasive detection of apoptosis represents a new tool that may yield a more definite diagnosis, a more accurate prognosis, and help improve therapies for human diseases. The intrinsic fluorescence of reduced nicotinamide adenine dinucleotide (NADH) may be a potential optical biomarker for the apoptosis detection because NADH is involved in the respiration for the mitochondrial membrane potential ( ) formation and adenosine-5'-triphosphate (ATP) synthesis, and the depletion of and ATP level is the hallmark of apoptosis. We have previously observed the NADH fluorescence lifetime change is associated with staurosporine (STS)-induced mitochondria-mediated apoptosis. However, its relationship with mitochondrial functions such as , ATP, and oxygen consumption rate is not clear. In this study, we investigated this relationship. Our results indicate that the NADH fluorescence lifetime increased when and ATP levels were equal to or higher than their values of controls and decreased before the depletion of and ATP, and the oxygen consumption rate did not change. These findings suggest that the increased NADH fluorescence lifetime in STS-induced cell death occurred before the depletion of and ATP and activation of caspase 3, and was not simply caused by cellular metabolic change. Furthermore, the NADH fluorescence lifetime change is associated with the pace of apoptosis. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Synthesis, radiolabeling, and in vivo evaluation of an 18F-labeled isatin analog for imaging caspase-3 activation in apoptosis

Cited by 101 publications

References 14 publications

Hybrid PET/MR Imaging of the Heart: Potential, Initial Experiences, and Future Prospects

Hybrid PET/MR Imaging of the Heart: Potential, Initial Experiences, and Future Prospects

In vivo imaging of apoptosis

Increase of reduced nicotinamide adenine dinucleotide fluorescence lifetime precedes mitochondrial dysfunction in staurosporine-induced apoptosis of HeLa cells

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