Synthesis of Ultrasensitive Magnetic Resonance Contrast Agents for Cancer Imaging Using PEG-Fatty Acid

Abstract

Diagnosis of cancer in the early stages requires sensitive magnetic resonance (MR) probes to detect low concentrations of magnetic substances. In this study, ultrasensitive magnetic resonance contrast agents (UMRCAs) composed of magnetic nanocrystals and amphiphilic block copolymers were synthesized for cancer detection using polyethylene glycol and fatty acid. The chemical structures and the compositions of PEGylated magnetic nanoparticles were analyzed. UMRCAs displayed remarkable colloidal stability and high sensitivity as MR probes. Furthermore, UMRCAs exhibited low cytotoxicity and excellent cancer detection ability in an in vivo animal model.

Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers

“…Fourthly, the liposomal barrier protects encapsulated pharmaceuticals from the degradative effects of the surrounding environment. 45 SPIONs can be loaded within the liposomes in two ways, ie, by incubating previously prepared liposomes and SPIONs under the influence of an external force resulting in localization of SPIONs within the hydrophilic core of liposomes, 46,47 or by directly precipitating the SPIONs within the hydrophilic core of liposomes, yielding highly uniform nanoparticles with sizes around 15 nm. 48 However, during coating, agglomerates of SPIONs sometimes get coated instead of discrete SPION cores being coated within a phospholipid shell.…”

Superparamagnetic iron oxide nanoparticles as a dual imaging probe for targeting hepatocytes in vivo

“…This problem limits their usefulness as an active targeting agent. Many researchers have focused on preventing aggregation of SPION in water and increasing blood circulation time (7,8). Furthermore, specific imaging studies using SPION for visualization of molecular events in vivo are an important field of research (8,9).…”

A systematic study of core size and coating thickness on manganese-doped nanocrystals for high T2 relaxivity as magnetic resonance contrast agent

“…The resulting product was filtered using a cellulose acetate syringe filter (pore size ≈ 200 nm) and dialyzed for two weeks against 10 mM sodium phosphate buffer (pH 7.4) using dialysis tube. saturation of the organic and continuous phases, the mixture was emulsified for 15 min with an ultrasonicator (ULH700S, Ulssohitech, Korea) at 300 W. After solvent evaporation in 4 h, the product was purified 3 times with centriprep at 3,000 rpm for 30 min to remove excess DA-PEG molecules [9].…”

“…The cells were incubated for 24 h with prepared MnMNCs, rinsed with 100 μL PBS (pH 7.4, 1 mM), and then treated with freshly prepared MTT solution (10 μL) and incubated for an additional 4 h before adding 100 μL dimethylsulfoxide. After 24 h, the plates were assayed using an enzyme-linked immunosorbent assay (Spectra Max 340, Molecular Devices, USA) and the results were measured at an absorbance wavelength of 575 nm and a reference wavelength of 650 nm [9].…”

“…And the coating molecules are important factor that would affect the nuclear relaxation of water protons by forming hydrogen bond. Therefore, it is necessary work to study the affect of coating materials even if their iron oxide core sizes are similar [8][9][10][11].…”