Synthesis of the Stationary Phase IS-Anionic (Internal Surface-Anionic) for Extraction of Ochratoxin A and B from Samples of Beers

Abstract

A new IS-anionic stationary phase was synthesized to make on-line extraction of the ochratoxin A and B from samples of beer for HPLC. The propyltriethylammonium chloride stationary phase was characterized affecting it’s elementary determination and RI specter, respectively. Evaluation of the IS-anionic column for the extraction and quantification of OTA and OTB in beer has shown that the column is suitable for efficient extraction (recovery >76.5%) and precise analysis. The detection limits for OTA and OTB were 0.03 and 0.07 μgL−1, respectively. The range of detector linearity was 0.03 at 20 μgL−1.

A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry

“…Although the procedures used to isolate and purify the OTA can differ substantially, the protocols to detect OTA in various fruits generally consist of extraction, purification, and finally OTA identification [ 15 ]. Usually, solid samples are extracted using acetonitrile-water, methanol-water or even chloroform to enhance the solubility and extraction efficiency of OTA [ 22 ]. In this study, methanol-water and dichloromethane were used as the extraction solvents.…”