Synthesis of the carbohydrate moiety from the parasite Echinococcus multilocularis and their antigenicity against human sera

Synthesis and Antigenicity against Human Sera of a Biotin-Labeled Oligosaccharide Portion of a Glycosphingolipid from the Parasite <i>Echinococcus multilocularis</i>

“…2). As reported previously, 6) compound F showed strong response to the AE group. On the contrary, although some of the patients' sera reacted strongly and the average of absorbance values of AE group (n=60) was significantly higher than that of the NH group (n=60) (p<0.001, Student's t-test), the average reactivity of compound 1 was not strong compared with that of F. However, comparison of the reactivity of individual serum against 1 and F revealed that each serum showed different reactivity to the oligosaccharides (Fig.…”

“…The average reactivity of F was stronger than that of 1, suggesting that the linear trisaccharide structure of F is important as a core structure for the antigenicity. 6) However, further studies will be necessary to elucidate structure-antigenicity relationship of oligosaccharides found in E. multilocularis.…”

“…Furthermore, Hülsmeier et al reported 22) that Em2, an antigen extracted from E. multilocularis, is a mucin-type glycoprotein. Based on these information, we synthesized four glycosphingolipids 12) and five carbohydrate structures of glycoproteins 6,8) of E. multilocularis, and examined antigenicity of the pure compounds by ELISA for their serodiagnostic potential. 5,6,23) Among the synthesized compounds (A-I), a glycosphingolipid, Galβ1 6(Fucα1 3) Galβ1 6Galβ1-Cer (D), and a biotinylated carbohydrate, Galα1 4Galβ1 3GalNAcα1-biotin-probe (F), showed good serodiagnostic potential for AE (Fig.…”

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] Our interests have been focused on the unique saccharide chain structure of GSLs and GPs found in several parasites, Echinococcus multilocularis, 5,6,8,12,16) Schistosoma mansoni, 3,7) Ascaris suum 13,14) and Toxocara canis. 4) Among them, we are strongly interested in the structures of GSLs and GPs from E. multilocularis.…”

Galα1-4Galβ1-3GalNAc is the dominant epitope of Em2 antigen, the mucin-type glycoprotein from Echinococcus multilocularis

“…Therefore, to confirm the correct sequence and serodiagnostic potential of the dominant epitope of Em2, we synthesized 11 O-glycans with possible structures and examined their antigenicity (Koizumi et al 2009;2011). In the ELISA utilizing these synthetic oligosaccharides as antigens, three biotinylated oligosaccharide probes, Galα1-4Galβ1-3GalNAcα1-R (R 0 biotinylated probe) (H), Galα1-4Galβ1-3(GlcNAcβ1-6)GalNAcα1-R (I), and Galβ1-3(Galα1-4Galβ1-4GlcNAcβ1-6) GalNAcα1-R (K), showed significant serodiagnostic potential in AE diagnosis.…”