Synthesis of terpolymers having a phospholipid polar group and poly(oxyethylene) in the side chain and their protein adsorption–resistance properties

Abstract

Terpolymers having phospholipid polar groups were synthesized from 2-methacryloyloxyethyl phosphorylcholine (MPC), methacryloyl or acryloyl poly(oxyethylene) macromonomers (POEM) [(CH 2 CH 2 O) n (where n ϭ 2-23); PEOM(2), PEOM(23), ME(9), Ph(6)], and n-butyl methacrylate (BMA). The characteristics of these terpolymer membranes were investigated by water content (H) and X-ray photoelectron spectroscopy. The content of water in the terpolymer increased with increasing content of MPC and length of oxyethylene units. The membranes of terpolymers were found to adsorb bovine serum albumin much less than those of poly(methyl methacrylate) and poly(BMA). Even though the contents of MPC in the terpolymer were 5 to 25 mol %, the terpolymer depressed BSA adsorption more than poly(MPC-co-BMA) consisting of 29 mol % of MPC. The use of terpolymer with POEM can decrease the amount of MPC in the polymer.

Protein-resistance performance of amphiphilic copolymer brushes consisting of fluorinated polymers and polyacrylamide grafted from silicon surfaces

“…The adsorption experiments were performed by BCA protein assay reagent. [30][31][32] Polymer-modied silicon wafers with 1 cm  1 cm area were immersed in PBS solution containing 2 mg mL À1 BSA or 1 mg mL À1 Fg. The adsorption was incubated in shaker at a constant temperature of 37 C for 24 h. Aer adsorption, the samples were taken out and rinsed with PBS three times to remove the non-adsorbed protein.…”

Synthesis and biocompatibility of phosphoryl polymer and relationship between biocompatibility and water structure

“…Ishihara and Nakayama groups had prepared a se rial of MPC modified polymers to improve their bio compatibility and hemocompartibility [14,15]. Oishi et al also reported that the polymers bearing a phos phorylcholine moiety had the excellent antithrombo genicity [16]. However, some of these materials showed poor mechanical properties and the limitation on the applications as blood contacting materials.…”

Synthesis of star polymer poly(ethylene glycol)3–poly(N,N‐dimethyl acrylamide) and its application in protein resistance and separation

“…One direct and effective way is the modification of the surface with protein‐resistant materials 3. Various polymer materials, such as heparin,8 dextran,9 polyoxazolines,10 poly(ethylene glycol) (PEG) or oligo(ethylene glycol),11, 12 and zwitterionic polymers,13–16 have been used to physically modify the surface. Particularly, PEG‐based materials with excellent nonfouling capabilities and biocompatibility have been used widely 17, 18.…”