Synthesis and biocompatibility of phosphoryl polymer and relationship between biocompatibility and water structure

Abstract

A series of copolymers, poly(methylmethacrylate co 2 methacryloyloxyethyl phosphorylcho line), with various compositions of methyl methacrylate (MMA) and 2 methacryloyloxyethyl phosphoryl choline (MPC) were synthesized by radical copolymerization in a mixed solvent of ethanol and chloroform. The structures of the copolymers were confirmed by proton nuclear magnetic resonance and elemental anal ysis. The properties and morphologies of the copolymers were characterized by differential scanning calorim eter, scanning electron microscopy, and optical microscope. The adsorption of bovine serum albumin (BSA) and the adhesion of platelet on the surfaces of the copolymer membrane significantly decreased with increas ing the MPC composition. The copolymers containing MPC above 18% showed excellent biocompatibility. Moreover, the relationship between the water structure and the biocompatibility was illustrated by changing quantity of the MPC in copolymers. The result showed that the amount of free water affected the platelet compatibility of the copolymer.

Two-Step Recrystallization of Water in Concentrated Aqueous Solution of Poly(ethylene glycol)

“…However, much interest has recently been shown in the relationship between the nonbiofouling property and recrystallization of water because of the finding by DSC of recrystallization of water in poly(2-methoxyethyl acrylate) (PMEA) , having excellent blood compatibility. In fact, DSC analysis revealed the existence of recrystallized water in some nonbiofouling polymers such as poly( N -vinyl pyrrolidone) (PVP), polysaccharides, − and poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), , although there was a difference between water-soluble polymers (PEG, PVP, polysaccharides, and PMPC) and nonwater-soluble polymer (PMEA). The recrystallized water was hypothesized to be the interfacial multilayered water molecules having a specific H-bonding structure that is recrystallizable and that prevents biofouling at the physiological T …”

Recent progress and perspectives in applications of 2-methacryloyloxyethyl phosphorylcholine polymers in biodevices at small scales

“…Hence, the investigation of the structure and behaviors of water in MPC polymers and their surfaces has been investigated by using techniques such as differential scanning calorimetry, 174,175 attenuated total reflection infrared spectroscopy, 176,177 and thermogravimetric analysis. 178 The use of these approaches can provide information of the water layer from the bulk, to micrometer, or even nanometer scales, but still faces great difficulty in further extending to a single molecule or atomic level to fully understand the details of the role of the water molecule in the MPC polymers. A combination of these conventional techniques with small nanodevices allowing the confinement of single molecules would provide new approaches to reach this goal.…”

The preparation of HEMA-MPC films for ocular drug delivery

“…As the relative percentage of MPC increased there was an increase in the bulk free water ratio compared to bound water. The increased amount of zwitterionic MPC pendent chain results in an increase in bulk water (Shi et al 2012;Chen et al 2010). The primary hydroxy group in the HEMA pendent chain can also form hydrogen bonds with water.…”

“…Hydrogel properties (e.g. swelling) and drug release profiles are frequently related to the water content within the gel (Lee et al 1975;Jhon & Andrade 1973;Shi et al 2012). Drug loading into the hydrogel is also an important parameter that can influence by the solvents that are used (Lewis et al 2008).…”