Synthesis of tentacle type magnetic beads as immobilized metal chelate affinity support for cytochrome c adsorption

Türkmen, Deniz; Yavuz, Handan; Deni̇zli̇, Adil

doi:10.1016/j.ijbiomac.2006.01.018

Cited by 49 publications

(12 citation statements)

References 40 publications

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“…The magnetic nanoparticles are ferromagnetic ones since the value for ferromagnetic ions is 3265G. The other parameter, g factor, is 1.4-3.1 and 2.0-9.7 for low-spin and high-spin complexes of magnetite (19). This factor was calculated using the following equation:…”

Section: Results and Discussion Characterization Of Nanoparticlesmentioning

confidence: 99%

Magnetic Nanoparticles for Plasmid DNA Purification through Hydrophobic Interaction Chromatography

Üzek

Özkara

Güngüneş

et al. 2014

Separation Science and Technology

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Magnetic poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-L-phenyl alanine), p(HEMA-co-MAPA) nanoparticles were produced by emulsion polymerization technique. The nanoparticles were characterized by zetasizer, atomic force microscopy (AFM), transmission electron microscopy (TEM), electron spin resonance spectroscopy (ESR), and Fourier transformed infrared spectroscopy (FTIR) techniques. Experimental conditions were optimized for DNA adsorption in aqueous media. The effects of several factors (i.e., temperature, pH, equilibrium concentration of DNA, salt type, and ionic strength) on adsorption capacity were examined in batch studies. The maximum DNA adsorption capacity was 211.3 mg/g for nanoparticles consisting of magnetite. The adsorption isotherms and kinetics were also examined. The Langmuir model and pseudo second-order kinetics are the best fitted to the data. Following the ten adsorption-desorption cycles, the decrease in adsorption capacity was only 12%. Finally, plasmid DNA was purified from E.coli lysate by magnetite nanoparticles consisting of MAPA. The purity and molecular mass of purified plasmid DNA were determined by the use of agarose gel electrophoresis resulting in 7000 base pairs for molecular mass and OC pDNA form following elution.

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Section: Results and Discussion Characterization Of Nanoparticlesmentioning

confidence: 99%

Magnetic Nanoparticles for Plasmid DNA Purification through Hydrophobic Interaction Chromatography

Üzek

Özkara

Güngüneş

et al. 2014

Separation Science and Technology

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“…mPoly(HEMA-EGDMA) beads were prepared by suspension polymerization in an aqueous medium as described in our previous article (33). HEMA was purchased from Sigma and was purified by vacuum distillation under a nitrogen atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Silane-Modified Magnetic Beads: Application to Immunoglobulin G Separation

et al. 2007

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The magnetic poly(2-hydroxyethyl methacrylate ethylene glycol dimethacrylate) [m-poly(HEMA-EGDMA)] beads (150-250-microm diameter in spherical form) were prepared by a radical suspension polymerization technique. The pseudo-specific ligand, reactive imidazole containing 3-(2-imidazoline-1-yl)propyl (triethoxysilane) (IMEO) was selected as a silanization agent. IMEO was covalently immobilized onto the magnetic beads. IMEO-immobilized m-poly(HEMA-EGDMA) beads were used for the affinity adsorption of immunoglobulin-G (IgG) from aqueous solutions and human plasma. To evaluate the degree of IMEO attachment, the m-poly(HEMA-EGDMA) beads were subjected to Si analysis by using flame atomizer atomic absorption spectrometer, and it was estimated as 36.6 mg IMEO/g of polymer. The nonspecific IgG adsorption onto the plain m-poly(HEMA-EGDMA) beads was very low (about 0.4 mg/g). Higher adsorption values (up to 55 mg/g) were obtained when the m-poly(HEMA-EGDMA)/IMEO beads were used from both aqueous solutions and human plasma. The maximum IgG adsorption on the m-poly(HEMA-EGDMA)-IMEO beads was observed at pH 7.0. The IgG molecules could be repeatedly adsorbed and desorbed with m-poly(HEMA-EGDMA)-IMEO beads without noticeable loss in the IgG adsorption capacity. The adsorption capacity from human plasma in magnetically stabilized fluidized bed decreased drastically from 78.9 to 19.6 mg/g with the increase of the flow rate from 0.2 to 3.5 mL/min.

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“…The magnetic carriers also decrease the cost of operation and supply (31)(32)(33)(34). Beside the providing solution for diffusion limitation, nanoparticles providing the increase of the adsorption capacity of the solid support are alternative for the macroporous microparticles (3,21,35,36). High surface area and lack of carrying limitations make the magnetic nanoparticles the desired choice for the purification of biomolecules (37).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Magnetic Polymeric Microparticles

Köse¹

2016

J. Turk. Chem. Soc., Sect. A: Chem.

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Abstract:The separation process is very important in a variety of scientific fields, especially in biochemistry and biotechnology. The separation performance of a method can be determined by the separation time and the purity of the separated molecule, which is directly proportional with the ligand chosen for the target molecule. The separation time is actually a very important step for the cost and the time-dependent analysis, especially in medical applications. Magnetic separation is a very advantageous technique because it eliminates the time-consuming sample preparation and centrifugation steps. However, the magnetic performance of adsorbent may not be strong enough to respond the magnetic force applied externally. Therefore, in nanoparticle studies, magnetic performance of nanoparticles is highly important. In this study, the magnetic performance of polymeric microparticles synthesized using different magnetic cores (Fe2O3 & Fe3O4, Fe & Ni, Ni & Co, Fe & Ni & Co) with a solid support, HEMA (2-hydroxyethylmethacrylate), and a functional monomer, AdeM (adenine methacrylate), were compared. Some other properties such as size, zeta potential, surface morphology, etc. were also studied.

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Synthesis of tentacle type magnetic beads as immobilized metal chelate affinity support for cytochrome c adsorption

Cited by 49 publications

References 40 publications

Magnetic Nanoparticles for Plasmid DNA Purification through Hydrophobic Interaction Chromatography

Magnetic Nanoparticles for Plasmid DNA Purification through Hydrophobic Interaction Chromatography

Silane-Modified Magnetic Beads: Application to Immunoglobulin G Separation

Characterization of Magnetic Polymeric Microparticles

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