Synthesis of silver nanoparticles using Bombyx mori silk fibroin and antibacterial activity

Harisha, K. S.; Shilpa, M.; Asha, S.; Parushuram, N.; Ranjana, R.; Narayana, B.; Sangappa, Y.

doi:10.1088/1757-899x/577/1/012008

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…Nanotechnology is an important field of applied science and technology dealing with design, synthesis and manipulation of particles whose structures range from approximately 1-100 nm [8,9]. In recent years, rapid advances have been made in the development of the VNp using different precursor materials such as shellac [10], iron oxide (Fe 3-δ O 4 magnetite nanoparticles) [11], silver nitrate (AgNO 3 ) [10], gold [12], a lipid matrix with antioxidant [13], porous silicon coupled to a peptide [14], chitosan [15], poly (lactic-co-glycolic acid) (PLGA) [16,17], lipid-polymer hybrid (glyceryl tripalmitate/Eudragit RS100) [18].…”

Section: Introductionmentioning

confidence: 99%

Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains

et al. 2020

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Bacterial infections represent one of the leading causes of mortality in the world. Among causative pathogens, S. aureus is prominently known as the underlying cause of many multidrug resistant infections that are often treated with the first-line choice antibiotic vancomycin (VCM). Loading antibiotics into polymeric nanoparticles (Np) displays promise as an alternative method to deliver therapy due to the greater access and accumulation of the antibiotic at the site of the infection as well as reducing toxicity, irritation and degradation. The aim of this work was to prepare, characterize and evaluate VCM-loaded nanoparticles (VNp) for use against S. aureus strains. Moreover, conjugation of Nps with holo-transferrin (h-Tf) was investigated as an approach for improving targeted drug delivery. VNp were prepared by double emulsion solvent evaporation method using PLGA and PVA or DMAB as surfactants. The particles were characterized for size distribution, Zeta Potential, morphology by transmission electron microscopy, encapsulation yield and protein conjugation efficiency. Process yield and drug loading were also investigated along with an in vitro evaluation of VNp antimicrobial effects against S. aureus strains. Results showed that Np were spontaneously formed with a mean diameter lower than 300 nm in a narrow size distribution that presented a spherical shape. The bioconjugation with h-Tf did not appear to increase the antimicrobial effect of VNp. However, non-bioconjugated Np presented a minimal inhibitory concentration lower than free VCM against a MRSA (Methicillin-resistant S. aureus) strain, and slightly higher against a 6 Author to whom any correspondence should be addressed.

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Section: Introductionmentioning

confidence: 99%

Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains

et al. 2020

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“…In summary, phenolic groups act as capping agents, stabilizing the AgNPs within the hydrogel by reducing Ag + to Ag 0 . Simultaneously, this process promotes the initiation of dityrosine crosslinking in photocrosslinkable environments [18,34].…”

Section: Resultsmentioning

confidence: 99%

Silver nitrate-assisted photo-crosslinking for enhancing the mechanical properties of an alginate/silk fibroin-based 3D scaffold

Moon,

Choi,

Cha

et al. 2024

Biofabrication

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Scaffolds play a pivotal role in tissue engineering and serve as vital biological substitutes providing structural support for cell adhesion and subsequent tissue development. An ideal scaffold must possess mechanical properties suitable for tissue function and exhibit biodegradability. Although synthetic polymer scaffolds offer high rigidity and elasticity owing to their reactive side groups, which facilitate tailored mechanical and rheological properties, they may lack biological cues and cause persistent side effects during degradation.To address these challenges, natural polymers have garnered attention owing to their inherent bioactivity and biocompatibility. However, natural polymers such as silk fibroin (SF) and tyramine-modified alginate–tyramine (AT) have limitations, including uncontrolled mechanical properties and weak structural integrity. In this study, we developed a blend of SF and AT as a printable biomaterial for extrusion-based 3D printing. The use of photocrosslinkable SF/AT inks facilitated the fabrication of complex scaffolds with high printability, thereby enhancing their structural stability. The incorporation of silver nitrate facilitated the tunability of mechanical and rheological behaviors. SF/AT scaffolds with varying stiffness in the physiologically relevant range for soft tissues (51–246 kPa) exhibited excellent biocompatibility, indicating their promising potential for diverse applications in tissue engineering.

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“…One of the most attractive plant proteins is silk fibroin. 76 Silk fibroin is an insoluble, biocompatible protein that is extracted from the silkworm Bombyx mori. The presence of tyrosine in the structure of this protein renders it a bioreducing agent for the preparation of inorganic nanomaterials.…”

Section: Biosynthesis Of Nanoparticlesmentioning

confidence: 99%

“…To date, an impressive array of plant proteins has been exploited for the preparation of nanosized materials. One of the most attractive plant proteins is silk fibroin . Silk fibroin is an insoluble, biocompatible protein that is extracted from the silkworm Bombyx mori.…”

Section: Biosynthesis Of Nanoparticlesmentioning

confidence: 99%

Biofabricated Nanostructures and Their Composites in Regenerative Medicine

Makvandi

Ghomi

Padil

et al. 2020

ACS Appl. Nano Mater.

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Biosynthesis of nanomaterials is gaining attention as a sustainable, environmentally friendly, and reliable method for manufacturing a extensive array of nanostructures, such as metal/metal oxides and organic and hybrid materials. Green synthesis is considered a crucial tool to reduce the harsh effects associated with conventional synthesis. Nanocomposite materials containing biosynthesized nanostructures are highly sought after in regenerative medicine. In the present Review, biosynthesis of metal/metal oxides and carbon-based nanomaterials using microorganisms (e.g., bacteria and fungi) and natural compounds (e.g., polysaccharides, proteins, fruit juices, and plant extracts) is highlighted. The toxicity of biosynthesized nanoparticles for biomedical application is also reviewed in depth. The applications of bionanocomposites prepared from these ecofriendly nanoparticles in tissue engineering are reviewed to provide readers with a background for future studies.

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Synthesis of silver nanoparticles using Bombyx mori silk fibroin and antibacterial activity

Cited by 8 publications

References 11 publications

Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains

Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains

Silver nitrate-assisted photo-crosslinking for enhancing the mechanical properties of an alginate/silk fibroin-based 3D scaffold

Biofabricated Nanostructures and Their Composites in Regenerative Medicine

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