Synthesis of silver nanoparticles using pine mushroom extract: A potential antimicrobial agent against E. coli and B. subtilis

Anthony, Kevin John Pulikotil; Muralidharan, M.; Jeyaraj, M.; Rathinam, Navanietha Krishnaraj; Gurunathan, Sangiliyandi

doi:10.1016/j.jiec.2013.10.008

Cited by 77 publications

(32 citation statements)

References 72 publications

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“…41 The observed band shift of the -OH and C-O groups, loss of existing C-O, and appearance of a new C-O peak suggest that both -OH and C-O groups of the extract are involved in the synthesis of AgNPs. 35 The results of this experiment suggest that the C-O group of amino acid residues in peptides and proteins has strong ability of binding metal; therefore, the proteins most likely form a layer on the AgNPs, which prevents agglomeration of the particles and thus, stabilizes the nanoparticles in the medium. 3,36,46,47 size distribution analysis of B-agNPs and F-agNPs Powers et al 48 proposed dynamic light scattering (DLS) as a useful technique for evaluating particle size and size distribution of nanomaterials in solution.…”

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confidence: 85%

“…35 Fresh mushrooms (5 g) were washed repeatedly with distilled water to remove any organic impurities. The cleaned mushrooms were then crushed to small pieces with a sterilized knife.…”

Section: Preparation Of Mushroom Extractmentioning

confidence: 99%

“…10 Both B-AgNPs and F-AgNPs represent a significant consensus with earlier findings reporting the synthesis of AgNPs using geranium leaf and mushroom extracts. 10,35,36,38,43 FTIr spectra of B-agNPs and F-agNPs FTIR spectra of the nanoparticles were recorded in order to identify the functional groups of biomolecules involved in the reduction and capping/stabilization of the synthesized nanoparticles.…”

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confidence: 99%

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Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Gurunathan¹,

Park²,

Han³

et al. 2015

IJN

270

151

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Background Recently, the use of nanotechnology has been expanding very rapidly in diverse areas of research, such as consumer products, energy, materials, and medicine. This is especially true in the area of nanomedicine, due to physicochemical properties, such as mechanical, chemical, magnetic, optical, and electrical properties, compared with bulk materials. The first goal of this study was to produce silver nanoparticles (AgNPs) using two different biological resources as reducing agents, Bacillus tequilensis and Calocybe indica. The second goal was to investigate the apoptotic potential of the as-prepared AgNPs in breast cancer cells. The final goal was to investigate the role of p53 in the cellular response elicited by AgNPs. Methods The synthesis and characterization of AgNPs were assessed by various analytical techniques, including ultraviolet-visible (UV-vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM). The apoptotic efficiency of AgNPs was confirmed using a series of assays, including cell viability, leakage of lactate dehydrogenase (LDH), production of reactive oxygen species (ROS), DNA fragmentation, mitochondrial membrane potential, and Western blot. Results The absorption spectrum of the yellow AgNPs showed the presence of nanoparticles. XRD and FTIR spectroscopy results confirmed the crystal structure and biomolecules involved in the synthesis of AgNPs. The AgNPs derived from bacteria and fungi showed distinguishable shapes, with an average size of 20 nm. Cell viability assays suggested a dose-dependent toxic effect of AgNPs, which was confirmed by leakage of LDH, activation of ROS, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells in MDA-MB-231 breast cancer cells. Western blot analyses revealed that AgNPs induce cellular apoptosis via activation of p53, p-Erk1/2, and caspase-3 signaling, and downregulation of Bcl-2. Cells pretreated with pifithrin-alpha were protected from p53-mediated AgNPs-induced toxicity. Conclusion We have demonstrated a simple approach for the synthesis of AgNPs using the novel strains B. tequilensis and C. indica , as well as their mechanism of cell death in a p53-dependent manner in MDA-MB-231 human breast cancer cells. The present findings could provide insight for the future development of a suitable anticancer drug, which may lead to the development of novel nanotherapeutic molecules for the treatment of cancers.

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confidence: 85%

“…35 Fresh mushrooms (5 g) were washed repeatedly with distilled water to remove any organic impurities. The cleaned mushrooms were then crushed to small pieces with a sterilized knife.…”

Section: Preparation Of Mushroom Extractmentioning

confidence: 99%

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confidence: 99%

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Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Gurunathan¹,

Park²,

Han³

et al. 2015

IJN

270

151

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“…The AgNPs synthesized by Acacia leucophloea extract had antibacterial activity against S. aureus [34]. AgNPs synthesized by pine mushroom ( Tricholoma matsutake ) extracts could be a potential antimicrobial agent against E. coli and B. subtilis [35]. Kim et al reported Gram-negative strains were more sensitive to AgNPs than Gram-positive strains because of their difference on membrane structure [36].…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Using Taxus yunnanensis Callus and Their Antibacterial Activity and Cytotoxicity in Human Cancer Cells

2016

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Plant constituents could act as chelating/reducing or capping agents for synthesis of silver nanoparticles (AgNPs). The green synthesis of AgNPs has been considered as an environmental friendly and cost-effective alternative to other fabrication methods. The present work described the biosynthesis of AgNPs using callus extracts from Taxus yunnanensis and evaluated their antibacterial activities in vitro and potential cytotoxicity in cancer cells. Callus extracts were able to reduce silver nitrate at 1 mM in 10 min. Transmission electron microscope (TEM) indicated the synthesized AgNPs were spherical with the size range from 6.4 to 27.2 nm. X-ray diffraction (XRD) confirmed the AgNPs were in the form of nanocrystals. Fourier transform infrared spectroscopy (FTIR) suggested phytochemicals in callus extracts were possible reducing and capping agents. The AgNPs exhibited effective inhibitory activity against all tested human pathogen bacteria and the inhibition against Gram-positive bacteria was stronger than that of Gram-negative bacteria. Furthermore, they exhibited stronger cytotoxic activity against human hepatoma SMMC-7721 cells and induced noticeable apoptosis in SMMC-7721 cells, but showed lower cytotoxic against normal human liver cells (HL-7702). Our results suggested that biosynthesized AgNPs could be an alternative measure in the field of antibacterial and anticancer therapeutics.

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“…However, the reducing reagents may increase the environmental toxicity or biological hazards . Moreover, plant‐mediated nanoparticle synthesis is preferred because it involves non‐toxic reagents, is cost‐effective, uses environmentally friendly solvents, is a single‐step method for biosynthesis process and is safe for humans and the environment.…”

Section: Introductionmentioning

confidence: 99%

Iron oxide nanoparticles coated with green tea extract as a novel magnetite reductant and stabilizer sorbent for silver ions: Synthetic application of Fe₃O₄@green tea/Ag nanoparticles as magnetically separable and reusable nanocatalyst for reduction of 4‐nitrophenol

Veisi

Ghorbani

2017

Applied Organom Chemis

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Green tea extract having many phenolic hydroxyl and carbonyl functional groups in its molecular framework can be used in the modification of Fe 3 O 4 nanoparticles. Moreover, the feasibility of complexation of polyphenols with silver ions in aqueous solution can improve the surface properties and capacity of the Fe 3 O 4 @green tea extract nanoparticles (Fe 3 O 4 @GTE NPs) for sorption and reduction of silver ions. Therefore, the novel Fe 3 O 4 @GTE NPs nano-sorbent has potential ability as both reducing and stabilizing agent for immobilization of silver nanoparticles to make a novel magnetic silver nanocatalyst (Fe 3 O 4 @GTE/Ag NPs). Inductively coupled plasma analysis, transmission and scanning electron microscopies, energy-dispersive X-ray and Fourier transform infrared spectroscopies, and vibrating sample magnetometry were used to characterize the catalyst. Fe 3 O 4 @GTE/Ag NPs shows high catalytic activity as a recyclable nanocatalyst for the reduction of 4-nitrophenol at room temperature.

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Synthesis of silver nanoparticles using pine mushroom extract: A potential antimicrobial agent against E. coli and B. subtilis

Cited by 77 publications

References 72 publications

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Biosynthesis of Silver Nanoparticles Using Taxus yunnanensis Callus and Their Antibacterial Activity and Cytotoxicity in Human Cancer Cells

Iron oxide nanoparticles coated with green tea extract as a novel magnetite reductant and stabilizer sorbent for silver ions: Synthetic application of Fe₃O₄@green tea/Ag nanoparticles as magnetically separable and reusable nanocatalyst for reduction of 4‐nitrophenol

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Synthesis of silver nanoparticles using pine mushroom extract: A potential antimicrobial agent against E. coli and B. subtilis

Cited by 77 publications

References 72 publications

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in&nbsp;MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in&nbsp;MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Biosynthesis of Silver Nanoparticles Using Taxus yunnanensis Callus and Their Antibacterial Activity and Cytotoxicity in Human Cancer Cells

Iron oxide nanoparticles coated with green tea extract as a novel magnetite reductant and stabilizer sorbent for silver ions: Synthetic application of Fe3O4@green tea/Ag nanoparticles as magnetically separable and reusable nanocatalyst for reduction of 4‐nitrophenol

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Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Iron oxide nanoparticles coated with green tea extract as a novel magnetite reductant and stabilizer sorbent for silver ions: Synthetic application of Fe₃O₄@green tea/Ag nanoparticles as magnetically separable and reusable nanocatalyst for reduction of 4‐nitrophenol