Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential

Abstract

Many pathogenic strains have acquired multidrug-resistant patterns in recent a year, which poses a major public health concern. The growing need for effective antimicrobial agents as novel therapies against multidrug-resistant pathogens has drawn scientist attention toward nanotechnology. Silver nanoparticles are considered capable of killing multidrug-resistant isolates due to their oligo-dynamic effect on microorganisms. In this research study NPs were synthesized using the gram-positive bacteria Lactobacillus bulgaricus and its activity against selected pathogenic strains. Lactobacillus bulgaricus pure cultures were isolated from raw milk and grown in “De Man, Rogasa, and Sharp” broth for synthesis of nanoparticles. Lactobacillus bulgaricus culture was centrifuged and Cell- free supernatant of it was employed with aqueous silvery ions and evaluated their antibacterial activities against bacterial strains i.e. Staphylococcus aureus, Staphylococcus epidermidis and Salmonella typhi using agar well diffusion assay. Antibiotic profiling against selected pathogenic strains were also conducted using disc diffusion method. The synthesis and characterization of silver nanoparticles were monitored primarily by the conversion of the pale-yellow color of the mixture into a dark-brown color and via ultraviolet-visible absorption spectroscopy and Scanning electron microscopy respectively. The result showed that that AgNPs with size (30.65-100 nm) obtained from Lactobacillus bulgaricus were found to exhibit antibacterial activities against selected bacterial strains. Taken together, these findings suggest that Lactobacillus bulgaricus has great potential for the production of AgNPs with antibacterial activities and highly effective in comparison to tested antibiotics.

Evaluation The Antibacterial Activity of Biosynthesis Silver Nanoparticles by Lactobacillus Gasseri Bacteria

“…(Table 1 and Figure 7). From the foregoing, it is clear that increasing the concentrations increases the rate of inhibition, and the antibacterial activity of AgNO3 and AgNPs above, it is clear that AgNPs were more effective as growth inhibitor for S. haemolyticus bacteria and K.pneomonaie bacteria in comparison to AgNO3; this is in agreement with previous studies [32,33].…”

Comparative Account of Biogenic Synthesis of Silver Nanoparticles Using Probiotics and Their Antimicrobial Activity Against Challenging Pathogens

“…Among different metallic nanoparticles, silver nanoparticles (AgNPs) are gaining attention as one of the most promising nanoparticles due to various unique properties it possesses [5]. AgNPs have large surface area to volume ratio and have strong toxic effect against wide range of microorganisms and can be used as a potential antimicrobial agent [6]. Silver nanoparticles synthesized using lactic acid bacteria (LAB), have been reported in different studies nowadays [7].…”

Brassica oleracea L. (Acephala Group) based zinc oxide nanoparticles and their efficacy as antibacterial agent

“…A variety of methods are available to synthesize the nanoparticles for their subsequent use in the antibacterial studies (Naseer et al, 2022). These methods include the synthesis of nanoparticles by employing the physical, chemical and biological treatments.…”