Synthesis of selenium-containing polysaccharides and evaluation of antioxidant activity in vitro

Wang, Junlong; Zhao, Baotang; Wang, Xiaofang; Yao, Jian; Zhang, Ji

doi:10.1016/j.ijbiomac.2012.08.011

Cited by 93 publications

(38 citation statements)

References 25 publications

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“…(969 μg/g) was significantly higher than that of SeASP MW4 (Table 1). Selenized ASP using conventional heating method exhibited an approximately 10-fold higher Se contents (the maximum Se content of 1703 μg/g) compared to SeASP MW [24].…”

Section: Discussionmentioning

confidence: 95%

“…The substitution position, molecular weight and solution conformation were characterized by employing 13 C NMR spectroscopy, size-exclusion chromatograph combined with multi-angle laser light scattering photometer (SEC-MALLS), congo red (CR) spectrophotometric analysis and atomic force microscopy (AFM) observation. After selenylation, SeASP exhibited better scavenge hydroxyl and superoxide radical activities in vitro [24]. In anti-tumor assays, SeASP displayed greater anti-proliferative effects against HepG-2 cells, A549 cells and Hela cells in a dose-dependent manner [25].…”

Section: Introductionmentioning

confidence: 95%

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Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

Wang

Yang

Bao

et al. 2017

International Journal of Biological Macromolecules

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In this work, selenylation of Artemisia sphaerocephala polysaccharide (SeASP) was studied by using HSeO/HNO/BaCl reaction system in microwave field. SeASP exhibited the Se content range of 111-264μg/g with high yields (72.1-94.9%). C NMR results indicated that the weak C-6 substitution was occurred. The decrease (from 7.348×10g/mol to 1.736-4.667×10g/mol) in weight average molecular mass (M) of SeASP was observed in size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS) analysis. SeASP exhibited a more rigid solution conformation which might be due to the degradation of polysaccharide chains in acidic reaction reagent. This was also supported by atomic force microscopy (AFM) result that SeASP showed short chains and island-like topography. In anti-tumor activity assays, SeASP exhibited the inhibition rates of 32.381% and 39.776% against human non-small cell lung cancer cell line (H1650) at the concentration of 100 and 200μg/mL, respectively. The relatively weak inhibition effect of SeASP was not related to cell apoptosis and cell cycle arrest, suggesting Se content might be a key factor to influence the anti-tumor activities of selenized polysaccharides in vitro.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 95%

Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

Wang

Yang

Bao

et al. 2017

International Journal of Biological Macromolecules

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“…concentrations and catalyst amount). The Se contents of SeASPs varied from 168 to 1703 μg/g (Wang et al, 2012). In the present study, it was important to study how MW or Se content affects its conformation in aqueous solution.…”

Section: Solution Conformation Of Seaspsmentioning

confidence: 99%

“…The preparation of selenized ASP (SeASP) and determination of selenium contents were reported in our previous report (Wang et al, 2012). Briefly, purified ASP was added in HNO3 solutions (the concentration of 0.4-1.2%) with stirring for 10 h. Then, different amounts of H2SeO3 and BaCl2 were added and reacted for 6 h. After the reaction, the mixture was neutralized (pH value was adjusted to 7-8 with 2 mol/L NaOH) and dialyzed (molecular weight cutoff 8-12 kDa) until the solution was colorless when ascorbic acid was added.…”

Section: Synthesis Of Selenized Aspmentioning

confidence: 99%

“…The C-6 substituted pattern and a decrease in MW were determined by using 13 C NMR spectroscopy and size-exclusion chromatograph combined with multi-angle laser light scattering photometer (SEC-MALLS). Compared to the native ASP, SeASP showed greater scavenge hydroxyl and superoxide radical activities in vitro (Wang et al, 2012). Moreover, some researchers have focused on the relations between conformation and biological activity of modified polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Wang

Bao

et al. 2016

Carbohydrate Polymers

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It has been reported in our previous work that selenized Artemisia sphaerocephala polysaccharides (SeASPs) with the Se content range of 168-1703μg/g were synthesized by using Na2SeO3/HNO3/BaCl2 system. In the present work, the solution property of SeASP was studied by using size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS). A decrease in df values indicated that SeASPs with different conformational features that were highly dependent on MW. SeASPs exhibited a more rigid conformation (df value of 1.29-1.52) in low molecular weight range (MW of 1.026-1.426×10(4)g/mol) and compact spherical conformation in high molecular weight range (MW of 2.268-4.363×10(4)g/mol). It could be due to the degradation of polysaccharide chains in HNO3, which was supported in monosaccharide composition analysis. Congo red (CR) spectrophotometric method and atomic force microscopy (AFM) results also confirmed the conformational transition and the evidence on the shape of the rigid chains. In vitro anti-tumor assays, SeASP2 displayed greater anti-proliferative effects against three tumor cell lines (hepatocellular carcinoma HepG-2 cells, lung adenocarcinom A549 cells and cervical squamous carcinoma Hela cells) in a dose-dependent manner. This suggested that selenylation could significantly enhance the anti-tumor activities of polysaccharide derivatives in vitro.

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Immunostimulatory and antioxidant activities of the selenized polysaccharide from edible Grifola frondosa

Zhu

et al. 2022

Food Science & Nutrition

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Grifola frondosa polysaccharide (GFP2) was extracted and purified by anion‐exchange chromatography. A selenized G. frondosa polysaccharide, SeGFP2, was modified in selenylation by nitric acid–sodium selenite (HNO3‐Na2SeO3) method. Structural features were investigated, and the lymphocyte proliferation and antioxidant activities were compared taking GFP2 as control. SeGFP2 with a molecular weight of 2.12 × 104 Da was composed of mannose, glucose, and galactose with a ratio of 3.5:11.8:1.0. A typical absorption of selenium ester was observed in SeGFP2 molecule. SeGFP2 was proposed as a branched polysaccharide, which consisted of 1,3‐D‐Glcp, 1,6‐D‐Glcp, 1,4,6‐D‐Galp, and 1,3,6‐D‐Manp. SeGFP2 showed a linear filamentous structure with some branches. SeGFP2 could significantly promote T‐ or B‐lymphocyte proliferation and the enhancement was higher than GFP2. The in vitro antioxidant activities of SeGFP2 were more potent than GFP2. These present data suggested that selenylation could significantly improve the lymphocyte proliferation and in vitro antioxidant activities of GFP2.

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Synthesis of selenium-containing polysaccharides and evaluation of antioxidant activity in vitro

Cited by 93 publications

References 25 publications

Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Immunostimulatory and antioxidant activities of the selenized polysaccharide from edible Grifola frondosa

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