Immunostimulatory and antioxidant activities of the selenized polysaccharide from edible Grifola frondosa

Abstract

Grifola frondosa polysaccharide (GFP2) was extracted and purified by anion‐exchange chromatography. A selenized G. frondosa polysaccharide, SeGFP2, was modified in selenylation by nitric acid–sodium selenite (HNO3‐Na2SeO3) method. Structural features were investigated, and the lymphocyte proliferation and antioxidant activities were compared taking GFP2 as control. SeGFP2 with a molecular weight of 2.12 × 104 Da was composed of mannose, glucose, and galactose with a ratio of 3.5:11.8:1.0. A typical absorption of selenium ester was observed in SeGFP2 molecule. SeGFP2 was proposed as a branched polysaccharide, which consisted of 1,3‐D‐Glcp, 1,6‐D‐Glcp, 1,4,6‐D‐Galp, and 1,3,6‐D‐Manp. SeGFP2 showed a linear filamentous structure with some branches. SeGFP2 could significantly promote T‐ or B‐lymphocyte proliferation and the enhancement was higher than GFP2. The in vitro antioxidant activities of SeGFP2 were more potent than GFP2. These present data suggested that selenylation could significantly improve the lymphocyte proliferation and in vitro antioxidant activities of GFP2.

Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium

“…GFP-A is mainly composed of Glc with α-type glycosidic linkages, whereas WGFP-A-a, AGFP-A-b, and AGFP-A-c are mainly composed of β-Glc p . Glucan GFPA (∼5,570 kDa) was extracted from the fruiting bodies of G. frondosa ( Li et al, 2022 ) and has an α-1,4-D-Glc p backbone with β-1,4,6-D-Glc p and T-β-Glc p as side chains. The mycelium glucans found in this study have lower molecular weights (4.1 kDa to ∼19.9 kDa) and are composed of a β-1,6-D-Glc p backbone with branched side chains of β-1,3-D-Glc p and T-D-Glc p residues at O-3 of the Glc p residues.…”

Isolation and identification of active components from Grifola frondosa and its anti‐EV71 virus effect

“…7 Grifola frondosa is a type of large medicinal and edible fungi, rich in polyphenols, sterols, proteins, polysaccharides and other active substances, and has many effects such as hypoglycemia, antitumor, anti-virus, anti-oxidantion and so on. 8,9 Previous reports have confirmed that G. frondosa has different degrees of inhibition on HIV virus, influenza virus and herpes simplex virus type I. 10 Therefore, G. frondosa is an important resource for the development of nutritional and functional foods with antiviral activity.…”

“…It has been reported that abundant secondary metabolites in edible and medicinal fungi shown antiviral effects and considered as an important source of viral inhibitors 7 . Grifola frondosa is a type of large medicinal and edible fungi, rich in polyphenols, sterols, proteins, polysaccharides and other active substances, and has many effects such as hypoglycemia, anti‐tumor, anti‐virus, anti‐oxidantion and so on 8,9 . Previous reports have confirmed that G. frondosa has different degrees of inhibition on HIV virus, influenza virus and herpes simplex virus type I 10 .…”