Synthesis of protein for DNA replication and cleavage events in the sand dollar embryo

Young, Charles W.; Hendler, F. J.; Karnofsky, David A.

doi:10.1016/0014-4827(69)90112-8

Cited by 41 publications

(6 citation statements)

References 18 publications

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“…Cells in older embryos appeared to undergo at most one division before mitotic arrest . These observations are consistent with a requirement for protein synthesis for entry of cells into mitosis (10,11) . Nuclei in embryos treated with 10 -4 M emetine for 30 min appeared larger and paler than those in normal embryos, but there was no other obvious cell damage .…”

Section: Ef Fect Of Emetine On Cell and Embryo Morphologysupporting

confidence: 85%

The Effect of Protein Synthesis Inhibition on the Entry of Messenger Rna Into the Cytoplasm of Sea Urchin Embryos

Hogan

Gross

1971

The Journal of Cell Biology

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Section: Ef Fect Of Emetine On Cell and Embryo Morphologysupporting

confidence: 85%

The Effect of Protein Synthesis Inhibition on the Entry of Messenger Rna Into the Cytoplasm of Sea Urchin Embryos

Hogan

Gross

1971

The Journal of Cell Biology

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“…The apparent insensitivity of protein synthesis at this stage to the inhibitor is probably related to the high resistance of early embryos to inhibitors in general, though it may be a characteristic of the present species (MANO 1970). Unlike the findings of BLACK et al 1967 andYOUNG et al 1969, in this work concentrations of inhibitor sufficient t o inhibit the second and third periods of DNA synthesis also prevented the first period of synthesis, while at lower concentrations of inhibitor no delay in initiation of DNA synthesis was observed. So, the fact that partial inhibition of protein synthesis causes delay in initiation of DNA synthesis suggests that DNA synthesis is not initiated until a definite amount of protein required for the initiation has been synthesized and accumulated.…”

Section: Discussioncontrasting

confidence: 50%

Dependence of Periodic Dna Synthesis on Protein Synthesis: Delay of Dna Synthesis in the Early Cleavage Stage of Sea Urchin Embryos Treated With Cycloheximide and Colchicine

Suzuki

Mano

1973

Dev Growth Differ

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The effects of cycloheximide and colchicine 011 cleavage and syntheses of DNA and proteins in cleaving embryos of the sea urchin, Hemicentrotus pulcherrimus, were examined. Cyclohexirnide caused delay of cell division with prolongation of the streak stage. Both inhibitors also caused delay in initiation o f DNA synthesis. The decrease in the rate and prolongation of the period of DNA synthesis caused by these inhibitors varied with their concentrations and the time of administration. Initiation o f DNA synthesis was' delayed when cycloheximide was added to suspensions of embryos between the time after preceding DNA synthesis terminated and a definite time before the predicted time of initiation of the next synthesis o f DNA, except at the stage of pronuclear fusion. However, when the inhibitor was added after initiation of the synthesis, the latter proceeded normally. Addition of 10 rnM cycloheximide immediately after fertilization or 2 mM cycloheximide 60 min before fertilization also delayed DNA synthesis at the stage o f pronuclear fusion, indicating that synthesis at this stage also required prior protein synthesis. Colchicine had less inhibitory effect on protein synthesis, but greatly delayed initiation o f DNA synthesis and prolonged its duration. These facts suggest that a definite amount of a particular protein must be synthesized and accumulated in each synthetic cycle before initiation o f DNA synthesis.In the early cleavage stage of sea urchin embryos DNA synthesis is known to occur periodically during the cell cycle (NEMER 1962, ZIMMERMAN 1963 1964). In pronuclei of sand dollar embryos incorporation of thymidine was also 'shown t o begin shortly before the fusion (SIMMEL and KARNOPSKY 1961).

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“…Puromycin inhibits protein synthesis and arrests cleavage at the streak stage in sea urchin eggs (Hultin, 1961). Cycloheximide also blocks cleavage in echinoderm eggs (Young et a/., 1969). Puromycin sensitivity is rapidly lost at about the time of the streak stage and the sensitivity period to puromycin is extended in the irradiated eggs (Rustad and Burchill, 1966) in which the streak stage is prolonged (Henshaw, 1940).…”

Section: Inducedmentioning

confidence: 99%

“…In addition to the synthesis of DNA and ATP, the first cleavage of echinoderm eggs appears to require protein synthesis (Hultin, 1961 ;Zotin, Milman and Faustov, 1965;Young, Hendler and Karnofsky, 1969). In order to further elucidate the mechanisms of ME action on cleavage, it is necessary to obtain more detailed information on the response of cells to ME during the cleavage cycles and on the relationship between protein synthesis required for division and the effects of ME.…”

mentioning

confidence: 99%

Delay and Quadripartition in Sea Urchin Eggs Induced by Short Exposure to 2-Mercaptoethanol

Yuyama¹

1971

The Biological Bulletin

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Synthesis of protein for DNA replication and cleavage events in the sand dollar embryo

Cited by 41 publications

References 18 publications

The Effect of Protein Synthesis Inhibition on the Entry of Messenger Rna Into the Cytoplasm of Sea Urchin Embryos

The Effect of Protein Synthesis Inhibition on the Entry of Messenger Rna Into the Cytoplasm of Sea Urchin Embryos

Dependence of Periodic Dna Synthesis on Protein Synthesis: Delay of Dna Synthesis in the Early Cleavage Stage of Sea Urchin Embryos Treated With Cycloheximide and Colchicine

Delay and Quadripartition in Sea Urchin Eggs Induced by Short Exposure to 2-Mercaptoethanol

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