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Although hydroxylamine, as such, is a product of normal cellular metabolism it is also a potent mutagen in vitro. However, in spite of this potential, it has not been shown to possess carcinogenic capabilities. Indeed, this chemical has demonstrated carcinostatic activity against certain tumors in animals. In addition, hydroxylamine has been shown to inactivate or inhibit a number of cellular enzymes and some viruses in vitro. It is also a skin irritant and sensitizer. It causes dermatitis and it is corrosive to the eyes. Acute and chronic exposures to hydroxylamine have caused methemoglobinemia and sulfhemoglobinemia.

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