Synthesis of phosphatidylcholine: An improved method without using the cadmium chloride complex of sn-glycero-3-phosphocholine

Ichihara, Kazuo; Iwasaki, Hitomi; Ueda, Kaori; Takizawa, Ryoko; Naito, Hideko; Tomosugi, Mitsuhiro

doi:10.1016/j.chemphyslip.2005.06.001

Cited by 43 publications

(35 citation statements)

References 31 publications

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“…The yield of LPC increased from 38.3 to 70.1 mol% when the molar ratio was increased from 1:6.25 to 1:50. Several studies have shown that a high excess of fatty acid over GPC is necessary to achieve sufficient conversion, and products of high purity (Adlercreutz, Lyberg, & Adlercreutz, 2003;Ichihara et al, 2005). However, if the concentration of the acyl donor is increased too much, a loss of lipase activity can occur by the inhibition of acidic substrate, which acidifies the microaqueous phase surrounding the lipase or causes desorption of water from the interface (Lee & Akoh, 1998;Yankah & Akoh, 2000).…”

Section: Molar Ratiomentioning

confidence: 98%

Enzymatic synthesis of lysophosphatidylcholine containing CLA from sn-glycero-3-phosphatidylcholine (GPC) under vacuum

Hong

Kim

et al. 2011

Food Chemistry

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Section: Molar Ratiomentioning

confidence: 98%

Enzymatic synthesis of lysophosphatidylcholine containing CLA from sn-glycero-3-phosphatidylcholine (GPC) under vacuum

Hong

Kim

et al. 2011

Food Chemistry

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“…Phosphatidylcholine (PC; dioleoyl) was synthesized according to Ref. 13 . Glass-distilled solvents were purchased from SigmaAldrich and Wako Pure Chemical Industries (Osaka, Japan).…”

Section: Reagentsmentioning

confidence: 99%

Preparation of fatty acid methyl esters for gas-liquid chromatography

Ichihara¹,

Fukubayashi²

2010

Journal of Lipid Research

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529

347

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“…Dioleoyl phosphatidylcholine (PC) was synthesized from sn-glycero-3-phosphocholine and oleic acid [17]. Acetone, hexane, and methanol were purchased from Wako Pure Chemical Industries as glass-distilled solvents for analysis of residual pesticides.…”

Section: Reagentsmentioning

confidence: 99%

Preparation of Fatty Acid Methyl Esters by Selective Methanolysis of Polar Glycerolipids

Ichihara

Yamaguchi

Araya

et al. 2010

Lipids

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KOH in aqueous methanol catalyzes selective methanolysis of polar glycerolipids with O-ester-linked acyl residues, while triacylglycerols and sterol esters are inert in the solution. Based on these findings, a convenient and reliable method was developed for the preparation of fatty acid methyl esters (FAMEs) from polar glycerolipids in lipid mixtures without prior isolation. Methanolysis of polar glycerolipids was completed within 2.5 min by vortexing or 20 min by shaking with 0.7 M KOH/70% (v/v) methanol in the presence of hexane at 30 degrees C. The yields of FAMEs obtained by the present method were greater than 95%. The method was applied successfully to gas chromatographic analysis of the fatty acid compositions of polar glycerolipids in seed oil and blood. No obvious differences were found between the fatty acid compositions determined by the present method and those determined by conventional methods, including lipid extraction with chloroform/methanol followed by isolation of polar lipids by chromatography. The fatty acid composition of polar glycerolipids, including phospholipids, can be determined readily in many crude samples.

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Synthesis of phosphatidylcholine: An improved method without using the cadmium chloride complex of sn-glycero-3-phosphocholine

Cited by 43 publications

References 31 publications

Enzymatic synthesis of lysophosphatidylcholine containing CLA from sn-glycero-3-phosphatidylcholine (GPC) under vacuum

Enzymatic synthesis of lysophosphatidylcholine containing CLA from sn-glycero-3-phosphatidylcholine (GPC) under vacuum

Preparation of fatty acid methyl esters for gas-liquid chromatography

Preparation of Fatty Acid Methyl Esters by Selective Methanolysis of Polar Glycerolipids

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