Preparation of fatty acid methyl esters for gas-liquid chromatography

Ichihara, Kazuo; Fukubayashi, Yumeto

doi:10.1194/jlr.d001065

Cited by 529 publications

(384 citation statements)

References 19 publications

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“…After that lipid extracts were separated by thinlayer chromatography in the system of solvents hexan : diethyl ether : glacial acetic acid (85 : 15 : 1) a fraction of free fatty acids was methylated with 3 М HCl in methanol [12].…”

Section: Methodsmentioning

confidence: 99%

The effect of N-stearoylethanolamine on plasma lipid composition in rats with experimental insulin resistance

Onopchenko¹

2015

Ukr.Biochem.J.

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Section: Methodsmentioning

confidence: 99%

The effect of N-stearoylethanolamine on plasma lipid composition in rats with experimental insulin resistance

Onopchenko¹

2015

Ukr.Biochem.J.

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Section: Preparation Of Fatty Acid Methyl Esters (Fame)mentioning

confidence: 99%

“…Transesterification with these reagents can be developed at room temperature in a short period of time. On the other hand, a disadvantage is that they do not convert free fatty acids in FAME, which limit its application to oils with high acidity (Simionato et al, 2010;Ichihara and Fukubayashi, 2010). For the analysis of CLA in samples having high levels of free fatty acids, Kramer et al (1997) reported a method using acid and base catalysis in sequence.…”

Section: Ajabsmentioning

confidence: 99%

“…The process of deriving the fatty acid analysis can be performed by acidic or basic catalysis (Eder, 1995;Millinsk et al, 2008;Simionato et al, 2010;Ichihara and Fukubayashi 2010;Millinsk et al, 2011). The methylation catalyzed by acid is commonly used because it is effective under many conditions and methyls all fatty acids, including the esterified and unesterified and those in salt form.…”

Section: Preparation Of Fatty Acid Methyl Esters (Fame)mentioning

confidence: 99%

“…The reagents commonly used in the transesterification of glycerides by basic catalysis is sodium hydroxide (NaOH) or potassium (KOH) in methanol (Millinsk et al, 2008;Ichihara and Fukubayashi, 2010) and sodium methoxide (NaOCH 3 ) in methanol (Millinsk et al, 2008). Transesterification with these reagents can be developed at room temperature in a short period of time.…”

Section: Ajabsmentioning

confidence: 99%

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Implications of Method Chosen for Analysis of Fatty Acids in Meat: A Review

Macedo¹,

Lacerda²,

Silva³

et al. 2012

American Journal of Agricultural and Biological Sciences

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The influence of the beef Fatty Acid (FA) profile on human health, has become a recurring concern. For an analysis of the lipid composition of food, it is necessary that the complex lipids be quantified in a suitable form for subsequent analysis of their composition by gas chromatography. The choice of appropriate methods of lipid extraction and esterification of fatty acids, is important in preventing chemical changes from within occurring in these molecules and the forming of artifacts. Among the methods used for quantification of lipids, those that use a mixture of polar and nonpolar solvents separate the lipids in the sample of the non-lipid components. As these methods were carried at ambient temperature, they most suitable for further analysis of the FA profile. These methods were extracted lipids efficiently and did not alter their chemical constitution. The methods of derivation of fatty acids for esterification could be catalyzed by acids or bases. The method was chosen to convert the lipids in Fatty Acid Methyl Esters (FAME) and allow their analysis by gas chromatography, should not lead to incomplete conversion of lipids into their FAME or form artifacts. The reagents commonly used in the transesterification of triacylglycerols in meat samples were the hydroxides of sodium or potassium hydroxide in methanol. Transesterification with these reagents could be developed at room temperature in a short period of time. It becomes a reliable technique for the identification of isomers of fatty acids such as conjugated linoleic acid. No single method performs optimally in all situations. The researcher must know the nature of the sample and select the appropriate method.

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