Synthesis of nitrogen-doped graphene nanosheets decorated with gold nanoparticles as an improved sensor for electrochemical determination of chloramphenicol
“…Therefore, monitoring of CAP levels in biological samples and food products is highly important. Various methods have been developed for this purpose, including chemiluminescence , voltammetry and chromatographic methods using fluorescence immunochromatography , gas chromatography–mass spectrometry (GC‐MS) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) .…”
A novel fluorescent nanosensor using molecularly imprinted silica nanospheres embedded CdTe quantum dots (CdTe@SiO2 @MIP) was developed for detection and quantification of chloramphenicol (CAP). The imprinted sensor was prepared by synthesis of molecularly imprinting polymer (MIP) on the hydrophilic CdTe quantum dots via reverse microemulsion method using small amounts of solvents. The resulting CdTe@SiO2 @MIP nanoparticles were characterized by fluorescence, UV-vis absorption and FT-IR spectroscopy and transmission electron microscopy. They preserved 48% of fluorescence quantum yield of the parent quantum dots. CAP remarkably quenched the fluorescence of prepared CdTe@SiO2 @MIP, probably via electron transfer mechanism. Under the optimal conditions, the relative fluorescence intensity of CdTe@SiO2 @MIP decreased with increasing CAP by a Stern-Volmer type equation in the concentration range of 40-500 µg L(-1). The corresponding detection limit was 5.0 µg L(-1). The intra-day and inter-day values for the precision of the proposed method were all <4%. The developed sensor had a good selectivity and was applied to determine CAP in spiked human and bovine serum and milk samples with satisfactory results.
“…Therefore, monitoring of CAP levels in biological samples and food products is highly important. Various methods have been developed for this purpose, including chemiluminescence , voltammetry and chromatographic methods using fluorescence immunochromatography , gas chromatography–mass spectrometry (GC‐MS) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) .…”
A novel fluorescent nanosensor using molecularly imprinted silica nanospheres embedded CdTe quantum dots (CdTe@SiO2 @MIP) was developed for detection and quantification of chloramphenicol (CAP). The imprinted sensor was prepared by synthesis of molecularly imprinting polymer (MIP) on the hydrophilic CdTe quantum dots via reverse microemulsion method using small amounts of solvents. The resulting CdTe@SiO2 @MIP nanoparticles were characterized by fluorescence, UV-vis absorption and FT-IR spectroscopy and transmission electron microscopy. They preserved 48% of fluorescence quantum yield of the parent quantum dots. CAP remarkably quenched the fluorescence of prepared CdTe@SiO2 @MIP, probably via electron transfer mechanism. Under the optimal conditions, the relative fluorescence intensity of CdTe@SiO2 @MIP decreased with increasing CAP by a Stern-Volmer type equation in the concentration range of 40-500 µg L(-1). The corresponding detection limit was 5.0 µg L(-1). The intra-day and inter-day values for the precision of the proposed method were all <4%. The developed sensor had a good selectivity and was applied to determine CAP in spiked human and bovine serum and milk samples with satisfactory results.
“…These traditional separation-based methods are sensitive, reliable and stable, but their high cost and complicated operation limit their application. Electrochemical biosensor has attracted considerable attention for its intrinsic advantages, such as, rapid response, ease to use, inexpensive instrumentation Pilehvar et al, 2012;Borowiec et al, 2013;Pilehvar et al, 2014), which can also be employed in simultaneous determination by using several signal tracers . However, to find high-sensitive and distinguishable multi-tracers is the key issue to achieve simultaneous analysis in the electrochemical assay.…”
“…Carbon‐based nanomaterials, including graphenes, carbon nanotubes (CNTs), nanowires, and nanoribbons, have been used as biomaterials. Among them, CNTs are generally classified as single‐walled, doubled‐walled, or multi‐walled structure nanotubes . Especially, CNTs show the remarkable ability to direct stem cell differentiation and could act as extracellular matrix to offer favorable environment to induce cellular attachment and growth .…”
Section: Introductionmentioning
confidence: 99%
“…It is known that nanoscale metals possess a better orientation as well as large and porous surface areas, and excellent conductivity. For example, gold nanoparticles (AuNPs) have been extensively applied in biomedicine owing to their large surface area, high surface free energy and good biocompatibility …”
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