Synthesis of naphthalimide-type chemsensor and its application in quality evaluation for polygonatum sibiricum Red

Abstract

The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM. This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation. The HAN was synthesized based on a CuI-catalyzed cross-coupling reaction in water, and then employed as a novel chemosensor that reacts with reducing sugars. Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min. The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81. The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP. Additionally, the HAN displayed fluorescence enhancement through “push–pull” mode, and fluorescence decreased through “pull–pull” mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.

Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum

“…( 13 , 21 , 51 – 54 ) were listed in Table 3 . With our interest in exploring TCMs related to anti-inflammatory ( 55 – 58 ), in particular Polygonatum species ( 22 , 23 ), we investigated the correlation between anti-inflammatory activity and the structure of Polygonatum polysaccharides. PKP possesses versatile bioactivity, including antioxidant activity, antidiabetic activity, antihyperlipidemic effect, etc., Glc and Gal are the main monosaccharide compositions.…”

“…However, the anti-inflammatory potential of P. kingianum polysaccharides (PKP) has rarely been studied. In our previous studies, the polysaccharide portion purified from the Polygonati species was mainly composed of glucose, glucuronic acid, galactose, and galacturonic acid ( 22 , 23 ).…”

Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua.

“…Following up on the above reports and continuing our interest in studying TCM (18)(19)(20)(21), particularly Polygonatum Rhizoma (22)(23)(24)(25), we reported a novel polysaccharide, PCP-F1, prepared by using the nine-steaming and nine-basking approach. By methylation analysis combined with Congo red assay, a detailed structure was determined.…”