A Novel Method for the Pre-Column Derivatization of Saccharides from Polygonatum cyrtonema Hua. by Integrating Lambert–Beer Law and Response Surface Methodology

Liu, Hui; Zhao, Yuanyuan; Chen, Leijing; Du, Jiao; Guo, Hongyan; Wang, Bin

doi:10.3390/molecules28052186

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…( 13 , 21 , 51 – 54 ) were listed in Table 3 . With our interest in exploring TCMs related to anti-inflammatory ( 55 – 58 ), in particular Polygonatum species ( 22 , 23 ), we investigated the correlation between anti-inflammatory activity and the structure of Polygonatum polysaccharides. PKP possesses versatile bioactivity, including antioxidant activity, antidiabetic activity, antihyperlipidemic effect, etc., Glc and Gal are the main monosaccharide compositions.…”

Section: Discussionmentioning

confidence: 99%

Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum

Wang

Liu

et al. 2023

Front. Nutr.

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IntroductionThis study aimed to investigate the structure characterization and antiinflammatory activity of a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl.MethodsWe isolated a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl. for the first time, which was then successively purified through hot-water extraction, 80% alcohol precipitation, anion exchange and gel permeation chromatography. The in vitro anti-inflammatory activity of PKP2-1 in MH7A cells was assessed using a CCK-8 kit assay.ResultsMonosaccharide composition assay revealed that PKP2-1 was mainly composed of glucose, galactose, mannose, and glucuronic acid at an approximate molar ratio of 6:2:2:1. It had a molecular weight of approximately 17.34 kDa. Structural investigation revealed that the backbone of PKP2-1 consisted of (→2, 3)-α-D-Galp(4→, →2)-α-D-Manp(3→, →2)-β-D-Glcp(4→) and α-D-Glcp(3→) residues with side chains (→2)-β-D-Glcp(4→, →1)-α-D-Galp(4→) and α-D-Glcp(3→) branches located at O-3 position of (→2, 3)-α-D-Galp(4→). The in vitro anti-inflammatory activity of PKP2-1 in MH7A cells revealed that PKP2-1 could reduce the expression of IL-11β and IL-6, increase the expression of IL-10 and induce apoptosis of synovial fibroblasts.ConclusionThe PKP2-1 could inhibit MH7A cell growth and potentially be exploited as an anti-inflammatory agent.

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Section: Discussionmentioning

confidence: 99%

Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum

Wang

Liu

et al. 2023

Front. Nutr.

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“…Following up on the above reports and continuing our interest in studying TCM (18)(19)(20)(21), particularly Polygonatum Rhizoma (22)(23)(24)(25), we reported a novel polysaccharide, PCP-F1, prepared by using the nine-steaming and nine-basking approach. By methylation analysis combined with Congo red assay, a detailed structure was determined.…”

Section: Introductionmentioning

confidence: 89%

Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua.

Zhao,

Wang,

et al. 2023

Front. Nutr.

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IntroductionPolygonatum cyrtonema Hua. (PC) is a traditional Chinese herb with a history of use in both food and medicine. For clinical use, processed PC pieces are most commonly used, while present research has focused on crude PC polysaccharides (PCPs).MethodsIn this study, a new polysaccharide, PCP-F1, with a molecular weight of 37.46 kDa, was separated from four-time processed PCPs by column chromatography and evaluated by antioxidant activity. It was composed of glucose, mannose, galactose, rhamnose, and galacturonic acid with a molar ratio of 3.5: 2.5: 1.3: 1.8: 0.8.Results and DiscussionThe methylation analysis and two-dimensional NMR measurement revealed that the configuration of PCP-F1 contained nine residues in the primary structural unit by the chain of →3)-α-D-Glcp, →2)-α-D-Glcp (6→, →1)-ꞵ-D-Glcp (2→, →2)-α-D-GalAp (3,4→, →1) -ꞵ-D-Manp (3→, →2)-α-D-Glcp (3→, branched for →3)-α-D-Glcp, →2)-ꞵ-D-Galp (4→, →1)-ꞵ-D-Glcp (2→, →2,4)-α-D-Manp (6→, →3)-α-L-Rhap (4→. Radical scavenging assays indicated that PCP-F1 could scavenge radicals with a high scavenging rate, suggesting PCP-F1 possesses good antioxidant activity. The study confirms the importance of processed PC and offers the potential for exploiting it as a functional food.

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“…Pre-column derivatization technology has garnered attention for its potential to improve the sensitivity of polysaccharide detection [15]. The application of HPLC, combined with pre-column derivatization, offers a new direction for addressing the challenges in polysaccharide analysis.…”

Section: Introductionmentioning

confidence: 99%

Pre-column Derivative HPLC and LC-Orbitrap-MS Analysis of Monosaccharides and Non-Polysaccharides in Polygonati Rhizoma

Liang,

Li,

Peng

et al. 2024

Preprint

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Polygonati Rhizoma, as a traditional medicinal herb, possesses pharmacological effects enhancing physical strength and immunity. In this study, a systematic analysis of the monosaccharide and non-polysaccharides components in Polygonati Rhizoma was conducted using pre-column derivatization high-performance liquid chromatography (HPLC-DAD) and liquid chromatography coupled to electrostatic orbitrap high-resolution mass spectrometry (LC-Orbitrap-MS) techniques. The polysaccharides from Polygonati Rhizoma were initially extracted, hydrolyzed, and derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP), resulting in the successful detection of five monosaccharides. The high sensitivity and specificity of the HPLC-DAD method were confirmed. Furthermore, by comparing the external standard method (ESM) and the quantitative analysis of multi-components by single-marker (QAMS) revealed that D-mannose is the most abundant monosaccharide in Polygonati Rhizoma. The LC-Orbitrap-MS analysis of Polygonati Rhizoma led to the identification of 53 compounds, including organic acids, amino acids, amides, saponins, alkaloids, esters, and others. This research provided significant data for the chemical composition analysis and the pharmacological basis study of Polygonati Rhizoma.

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A Novel Method for the Pre-Column Derivatization of Saccharides from Polygonatum cyrtonema Hua. by Integrating Lambert–Beer Law and Response Surface Methodology

Cited by 4 publications

References 33 publications

Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum

Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum

Structural characterization and antioxidant activity of processed polysaccharides PCP-F1 from Polygonatum cyrtonema Hua.

Pre-column Derivative HPLC and LC-Orbitrap-MS Analysis of Monosaccharides and Non-Polysaccharides in Polygonati Rhizoma

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