Synthesis of L-asparaginase bySerratia marcescens (Nima)

Sukumaran, C. P.; Singh, D. V.; Mahadevan, P. R.

doi:10.1007/bf02716875

Cited by 20 publications

(16 citation statements)

References 10 publications

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“…It is generally accepted that the synthesis of L-asparaginase is under catabolic repression in the case of E. coli and Erwinia aeroideae at higher concentration (Liu and Zajic, 1972;Jeffries, 1976). Glucose was reported as the best carbon source under aerobic conditions for synthesis of L-asparaginase by Serratia marcescens (Sukumaran et al, 1979). In contrast the L-asparaginase activity was increased when the gram-positive bacteria grown on nutritional conditions in which the nitrogen source was apparently the limiting factor for growth, where as the L-asparaginase activity was decreased when the limiting for growth is carbon source (Golden and Bernlohr, 1985).…”

Section: Influence Of Different Carbon Sources On L-asparaginase Prodmentioning

confidence: 95%

Production of L-asparaginase from Natural Substrates by Aspergillus terreus MTCC 1782: Optimization of Carbon Source and Operating Conditions

Baskar¹,

Renganathan²

2011

International Journal of Chemical Reactor Engineering

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In the present work, the effect of different carbon sources, namely glucose, sucrose, maltose, fructose and lactose, was studied for extracellular L-asparaginase production by Aspergillus terreus MTCC 1782 in submerged fermentation. The best carbon source and operating conditions such as initial pH, inoculum size, temperature and agitation rate were optimized. Glucose was found to be the best carbon source for L-asparaginase production using modified Czapek-Dox media containing L-proline as substrate. Sucrose was found to be the best carbon source for L-asparaginase production using modified Czapek-Dox media containing groundnut oil cake flour as substrate. Glucose was found to be the best carbon source for maximum L-asparaginase production using modified Czapek-Dox media containing soya bean meal flour as substrate. The soya bean meal flour was found to be the best natural substrate for maximum L-asparaginase activity of 35.3 IU/mL using 0.6% glucose as carbon source at the optimal culture conditions of initial pH 6, inoculum size 2%, temperature 35 • C and agitation rate 160 rpm.

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Section: Influence Of Different Carbon Sources On L-asparaginase Prodmentioning

confidence: 95%

Production of L-asparaginase from Natural Substrates by Aspergillus terreus MTCC 1782: Optimization of Carbon Source and Operating Conditions

Baskar¹,

Renganathan²

2011

International Journal of Chemical Reactor Engineering

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“…The present study finds limitation in understanding the exact role of oxalate supplementation in subsequent increase in organoleptic characteristic as well as in production yield. However, oxalate has been recorded to promote L-asparginase synthesis (Sukumaran et al, 1979) and the formation of amino acid anhydrides of oxalyl compounds (Worthington et al 1962) in host bacterium which may in turn play some role in enhanced production of prodigiosin. …”

Section: Quantification Purification and Chemical Characterization Omentioning

confidence: 99%

Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia marcescens OX_R strain

Suryavanshi¹,

Waghmode²,

Bharti³

et al. 2016

J App Pharm Sci

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Prodigiosin a multifaceted secondary metabolite produced by Serratia spp. having great potential as a pharmaceutical. In the present study we demonstrate that oxalate supplementation in peptone glycerol production media increased organoleptic characters and yield of prodigiosin pigment extracted from oxalotrophic Serratia marcescens OX_R isolated from Indian bat guano sample. The pigment was demonstrated in-vitro as an antibacterial agent against common opportunistic skin surface pathogen Staphylococcus aureus NCIM 5021 strain as killing activity by agar well diffusion method. The docking analysis and pharmacophore modelling indicated that the probable mechanism of action of the prodigiosin was against Staphylococcus aureus DNA gyrase protein. The pigment was also found to efficiently dye both cotton and latex polymer. In summary, we describe here an oxalotrophic Serratia marcescens which may serve as a potent and economical resource of prodigiosin which owing to its dyeing and anti-bacterial activities finds future avenues to be developed as dressing material for nosocomial subjects or burn victim patients.

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“…The stationary phase was prolonged up to 72hrs showed the maximum yield of biomass. L-asparaginase synthesis was increased by addition of L-aspartic acid, L-glutamine, L-glutamic acid and L-asparagine to the level of 20, 20.7, 19.8 and 25 IU/ 10ml respectively using Serratia marcescens reported by Sugumaran et al [21].The addition of L-aspartic acid or L-glutamic acid to the medium containing sodium fumarate and corn steep liquor slightly enhanced enzyme production, but these amino acids may not be considered specific inducers for enzyme formation [22]. At 5.0% Yeast Extract concentration, the higher aeration provided yields of 5.2 IU/ml in this experiment.…”

Section: Discussionmentioning

confidence: 82%

Effect of Inducers and Physical Parameters on the Production Of L-Asparaginase Using Aspergillus Terreus

Rajesh¹,

Rajesh²,

veni³

et al. 2011

J Bioproces Biotechniq

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An effort has been made to find the best medium and operating conditions for the production of extracellular L-asparaginase by Aspergillus terreus in shake culture fermentation through stepwise optimization strategies like studies on the effect of pH and temperature on L-asparaginase, studies on the enzyme activity in various concentration of L-glutamine and study the growth kinetics for L-glutamine medium. Our study shows that L-glutamine combined with L-asparagine influence the asparaginase production level and physical parameter such as pH and temperature has more contribution in enzyme production. The pH 8 and temperature 35°C found to be good environmental condition the production of extracellular L-asparaginase by Aspergillus terreus.

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Synthesis of L-asparaginase bySerratia marcescens (Nima)

Cited by 20 publications

References 10 publications

Production of L-asparaginase from Natural Substrates by Aspergillus terreus MTCC 1782: Optimization of Carbon Source and Operating Conditions

Production of L-asparaginase from Natural Substrates by Aspergillus terreus MTCC 1782: Optimization of Carbon Source and Operating Conditions

Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia marcescens OX_R strain

Effect of Inducers and Physical Parameters on the Production Of L-Asparaginase Using Aspergillus Terreus

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