Effect of Inducers and Physical Parameters on the Production Of L-Asparaginase Using Aspergillus Terreus

Abstract

An effort has been made to find the best medium and operating conditions for the production of extracellular L-asparaginase by Aspergillus terreus in shake culture fermentation through stepwise optimization strategies like studies on the effect of pH and temperature on L-asparaginase, studies on the enzyme activity in various concentration of L-glutamine and study the growth kinetics for L-glutamine medium. Our study shows that L-glutamine combined with L-asparagine influence the asparaginase production level and physical parameter such as pH and temperature has more contribution in enzyme production. The pH 8 and temperature 35°C found to be good environmental condition the production of extracellular L-asparaginase by Aspergillus terreus.

Isolation, purification and characterization of an extracellular L-asparaginase produced by a newly isolated <i>Bacillus megaterium</i> strain MG1 from the water bodies of Moraghat forest, Jalpaiguri, India

“…Moreover, previous studies also indi- cated that L-asparagine can induce L-asparaginase production, though different bacteria use different control mechanisms at the gene level. Rajesh et al (2011) showed that L-asparagine induced Aspergillus terreus to produce more asparaginase enzyme. The ans operon in Rhizobium etli was also reported to carry an asparagine permease gene, suggesting that the operon's expression is determined by the level of asparagine in the cell and it is probable that regulation is mainly at a transcriptional level, asparagine being the inducer (Ortuño-Olea and Durán-Vargas, 2000).…”

Dietary Fermented Rice Bran Is an Effective Modulator of Ulcerative Colitis in Experimental Animal

“…A number of fermentation studies on rice bran indicated that the process is capable of producing short-chain fatty acids such as acetic acid, propionic acid, butyric acid, lactic acid, enzymes such as protease amylase, phenolic compound, and antioxidant compound like vitamin E as well as secondary metabolites such as griseofulvin, etc. for treatment of UC [67][68][69][70][71][72][73][74]. Previous studies also indicated that it is possible to change the lipid and phospholipid composition and phenolic acid content and antioxidant activities of rice bran would be the effect modulators for the treatment of UC [75][76].…”

The IN VITRO STUDY OF APOPTOSIS INDUCTION BY L-ASPARAGINASE OF ASPERGILLUS FUMIGATUS (ASPF-6) ON HUMAN LEUKEMIA CELL LINES

“…Extracellular matrix was removed using Trypsin and it is continued for 2-4 mine in CO 2 incubator. After trypsinization cells were suspended in fresh serum to neutralize the trypsin [13]. The solution subjected to centrifugation at 25,000 rpm/4 min and the pellet was collected.…”