Synthesis of high 4-hydroxybutyrate copolymer by Cupriavidus sp. transformants using one-stage cultivation and mixed precursor substrates strategy

Syafiq, Ishak Muhammad; Huong, Kai‐Hee; Shantini, K.; Vigneswari, Sevakumaran; Aziz, Nursolehah Abd; Amirul, Al-Ashraf Abdullah; Bhubalan, Kesaven

doi:10.1016/j.enzmictec.2016.11.011

Cited by 18 publications

(18 citation statements)

References 28 publications

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“…Co-feeding of soybean oil and γ-butyrolactone produces high yield of copolymer accumulation, although higher supplementation of the precursor has been shown to increase the 4HB fraction while inhibiting cell growth (Park and Kim, 2011). High PHA density fed-batch cultivation strategies employing mixed precursors (5:1 ratios 1,4-butanediol and 1,6-hexanediol) have been developed to boost the 4HB content up to 99 mol% in Cupriavidus malaysiensis transformed with additional copies of PHA synthase gene (PhaC) (Norhafini et al, 2017(Norhafini et al, , 2019Syafiq et al, 2017).…”

Section: Poly (3-hydroxybutyrate-co-4-hydroxybutyrate)mentioning

confidence: 99%

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Turco

Santagata

Corrado

et al. 2021

Front. Bioeng. Biotechnol.

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The transition toward “green” alternatives to petroleum-based plastics is driven by the need for “drop-in” replacement materials able to combine characteristics of existing plastics with biodegradability and renewability features. Promising alternatives are the polyhydroxyalkanoates (PHAs), microbial biodegradable polyesters produced by a wide range of microorganisms as carbon, energy, and redox storage material, displaying properties very close to fossil-fuel-derived polyolefins. Among PHAs, polyhydroxybutyrate (PHB) is by far the most well-studied polymer. PHB is a thermoplastic polyester, with very narrow processability window, due to very low resistance to thermal degradation. Since the melting temperature of PHB is around 170–180°C, the processing temperature should be at least 180–190°C. The thermal degradation of PHB at these temperatures proceeds very quickly, causing a rapid decrease in its molecular weight. Moreover, due to its high crystallinity, PHB is stiff and brittle resulting in very poor mechanical properties with low extension at break, which limits its range of application. A further limit to the effective exploitation of these polymers is related to their production costs, which is mostly affected by the costs of the starting feedstocks. Since the first identification of PHB, researchers have faced these issues, and several strategies to improve the processability and reduce brittleness of this polymer have been developed. These approaches range from the in vivo synthesis of PHA copolymers, to the enhancement of post-synthesis PHB-based material performances, thus the addition of additives and plasticizers, acting on the crystallization process as well as on polymer glass transition temperature. In addition, reactive polymer blending with other bio-based polymers represents a versatile approach to modulate polymer properties while preserving its biodegradability. This review examines the state of the art of PHA processing, shedding light on the green and cost-effective tailored strategies aimed at modulating and optimizing polymer performances. Pioneering examples in this field will be examined, and prospects and challenges for their exploitation will be presented. Furthermore, since the establishment of a PHA-based industry passes through the designing of cost-competitive production processes, this review will inspect reported examples assessing this economic aspect, examining the most recent progresses toward process sustainability.

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Section: Poly (3-hydroxybutyrate-co-4-hydroxybutyrate)mentioning

confidence: 99%

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Turco

Santagata

Corrado

et al. 2021

Front. Bioeng. Biotechnol.

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“…The bacteria strain of Cupriavidus malaysiensis USMAA1020 transformant harboring additional PHA synthase gene from Cupriavidus malaysiensis USMAA2-4, was used to produce P(3HB-co-4HB) copolymer in a 15 L bioreactor with a total working volume of 10 L as previously described (Syafiq et al, 2017). A mixture of carbon precursors (0.75 wt% C) with both 1,4-butanediol (0.625 wt% C) and 1,6-hexanediol (0.125 wt% C) were used for the biosynthesis of P(3HB-co-4HB).…”

Section: Biosynthesis Of P(3hb-co-4hb)mentioning

confidence: 99%

“…Removal of endotoxins were done using hydrogen peroxide (Vigneswari et al, 2015). The gas chromatography (GC) analysis was carried out to determine the PHA composition and content in the lyophilized cells based on a study done by Braunegg et al (1978) with some modification, using Shimadzu GC-17A (Shimadzu, Japan) as described by Syafiq et al (2017).…”

Section: Biosynthesis Of P(3hb-co-4hb)mentioning

confidence: 99%

Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation

Vigneswari

Chai

Kamarudin

et al. 2020

Front. Bioeng. Biotechnol.

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Biomaterial scaffolds play crucial role to promote cell proliferation and foster the regeneration of new tissues. The progress in material science has paved the way for the generation of ingenious biomaterials. However, these biomaterials require further optimization to be effectively used in existing clinical treatments. It is crucial to develop biomaterials which mimics structure that can be actively involved in delivering signals to cells for the formation of the regenerated tissue. In this research we nanoengineered a functional scaffold to support the proliferation of myoblast cells. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer is chosen as scaffold material owing to its desirable mechanical and physical properties combined with good biocompatibility, thus eliciting appropriate host tissue responses. In this study P(3HB-co-4HB) copolymer was biosynthesized using Cupriavidus malaysiensis USMAA1020 transformant harboring additional PHA synthase gene, and the viability of a novel P(3HB-co-4HB) electrospun nanofiber scaffold, surface functionalized with RGD peptides, was explored. In order to immobilize RGD peptides molecules onto the P(3HB-co-4HB) nanofibers surface, an aminolysis reaction was performed. The nanoengineered scaffolds were characterized using SEM, organic elemental analysis (CHN analysis), FTIR, surface wettability and their in vitro degradation behavior was evaluated. The cell culture study using H9c2 myoblast cells was conducted to assess the in vitro cellular response of the engineered scaffold. Our results demonstrated that nano-P(3HB-co-4HB)-RGD scaffold possessed an average fiber diameter distribution between 200 and 300 nm, closely biomimicking, from a morphological point of view, the structural ECM components, thus acting as potential ECM analogs. This study indicates that the surface conjugation of biomimetic RGD peptide to the nano-P(3HB-co-4HB)

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“…Біосинтез інших мікробних метаболітів на змішаних субстратах. Стратегія використання суміші субстратів використовується не лише для отримання ПАР, а й для інших практично цінних мікробних метаболітів: ліпідів [16][17][18][19][20][21][22][23][24], екзополісахаридів [25][26][27], пілігідроксиалканоатів [28][29][30], ферментів [31][32][33], органічних кислот [34][35][36][37][38], пропандіолу [39,40], біоетанолу [41], біоводню [42,43].…”

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“…У наступних дослідженнях [29] автори встановили можливість підвищення концентрації синтезованого на суміші 1,4-бутандіолу і 1,6-гександіолу сополімеру до 8,6 г/л (вміст 4HB 35%). Крім того, у [30] було показано можливість отримання сополімеру на суміші γ-бутиролактону та 1,6-гександіолу з вмістом 4HB 92% за рахунок використання генно модифікованих штамів Cupriavidus sp. USMAA1020 phaC2-4 та Cupriavidus sp.…”

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Intensification of Synthesis of Practically Important Microbial Metabolites on Substrates Mixture

Пирог¹,

Gershtman²,

Penchuk³

2018

SWNUFT

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Mixture of growth substrates Increasing the efficiency of microbial technology Cultivation of microorganisms on mixture of growth substrates allows to avoid unproductive loss of carbon and energy that occurs when using monosubstrates, and also to improve efficiency of transformation of carbon substrates into biomass and to intensify synthesis of secondary metabolites. The paper analyzes the modern scientific literature of last two or five years concerning the increase of synthesis on the mixed substrates (including industrial waste) of primary (organic acids, lipids, enzymes), secondary (polyhydroxyalkanoates, polysaccharides, surface-active substances) metabolites, and also bioethanol and biohydrogen. Using mixture of substrates in microbial technologies enables to increase the rates of synthesis of practically valuable metabolites by 1.5-10 times compared with cultivation producers on corresponding monosubstrates, and in some cases even regulate the composition and properties of final product. Own experimental data on synthesis intensification on mixture of industrial waste (frying sunflower oil, waste of biodiesel production, molasses) of microbial exopolysaccharide ethapolan (producer Acinetobacter sp. IMV B-7005) and Nocardia vaccinii IMV B-7405 surfactants are presented. Unlike most scientists who empirically establish both the concentration of substrates in the mixture and the choice of monosubstrates, in our studies to increase the carbon transformation of substrates mixture into final product, the molar ratio of monosubstrate concentrations in mixture was established on the basis of theoretical calculations of the energy requirement for process biosynthesis. In addition, using mixture of waste to obtain microbial surfactants will not only reduce cost of final product, but also utilize toxic industrial waste and increase profitability of biodiesel production.

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Synthesis of high 4-hydroxybutyrate copolymer by Cupriavidus sp. transformants using one-stage cultivation and mixed precursor substrates strategy

Cited by 18 publications

References 28 publications

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation

Intensification of Synthesis of Practically Important Microbial Metabolites on Substrates Mixture

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