Synthesis of gold nanoparticles derived from mannosylerythritol lipid and evaluation of their bioactivities

Abstract

In this study, we introduce a simple and green method for synthesis of gold nanoparticles (AuNPs) using microbial glycolipid mannosylerythritol lipid (MEL) produced from Ustilago maydis CGMCC 5.203 and to evaluate their biomedical activities. MEL was found 10.3 g/L using sunflower oil. The formation of MEL-AuNPs was verified using UV–visible spectrum, XRD, TEM, FTIR, SEM, and EDX. In the biomedical examinations, MEL-AuNPs demonstrated potential cytotoxicity against HepG2 cells, and IC 50 values were found to be 100 and 75 µg/mL for 24 h and 48 h of exposure, respectively, which indicates its good performance against cancer cells. The IC 50 value of MEL-AuNPs was found to be 115 and 124 µg/mL for DPPH and ABTS scavenging activities, respectively. The biosynthesized MEL-AuNPs significantly inhibited cell growth of pathogenic Gram-positive and Gram-negative bacteria. These findings indicated that MEL plays a crucial role in the rapid biofabrication method of metallic NPs possessed the potential of biomedical activities.

AuNPs composites of gelatin hydrogels crosslinked by ferrocene‐containing polymer as recyclable supported catalysts

“…The appearance of these peaks undisputedly demonstrates the successful formation of AuNPs in AuNPs‐HG‐1 . Notably, the reflection peak at 38.23° has higher intensity than other reflection peaks at 44.38°, 64.52°, and 77.55°, showing good accordance with the previous reports . The estimation of crystalline size ( D ) of AuNPs‐HG‐1 was then conducted by using Debye–Scherrer equation [ D = (0.9 λ )/ β cos θ ] that reveals the relationship between diffraction peaks broadening and crystalline size .…”

Synergistic antibacterial and antibiofilm effects of ultrasound and MEL-A against methicillin-resistant Staphylococcus aureus

“…Our previous researches also revealed that MEL-A has significantly inhibited the growth of Bacillus cereus [12] and Staphylococcus aureus [13] . Expect for its intrinsic capacity, MELs have been a key part of nanoparticles as emulsifier, exhibiting potent inhibitive effect on pathogens [30] , [31] , [32] . However, the abuse of antibiotics resulted in the emergence of methicillin-resistant Staphylococcus aureus (MRSA), which can survive in severe environment and convey a novel challenge to anti-infective treatments [33] .…”

Contributions of Glycolipid Biosurfactants and Glycolipid-Modified Materials to Antimicrobial Strategy: A Review

“…These findings indicated that MELs play a crucial role in the rapid biosynthesis of metallic nanoparticles and enhance the antimicrobial property, yet the underlying mechanism of inhibition still needs further study. metallic nanomaterials including silver, zinc oxide, and gold nanoparticles mediated by MEL-A, and all of them exert inhibitory efficacy against pathogenic gram-positive and gram-negative bacteria [104]. These findings indicated that MELs play a crucial role in the rapid biosynthesis of metallic nanoparticles and enhance the antimicrobial property, yet the underlying mechanism of inhibition still needs further study.…”