In the second stage of our study concerning the search for new antibacterial rifamycin antibiotics, reaction of the aldehydes 3‐formylrifamycin SV (1) and 25‐O‐deacetyl‐3‐formylrifamycin SV with ammonia and acetone has been investigated. A new synthetic method for the preparation of a new group of rifamycin derivatives with a cyclic substituent at C‐3 having a 4‐piperidone structure, represented by compounds 6a, 6b, and 7a, has been developed. The structures of the compounds and a reaction mechanism have been proposed on the basis of mass spectrometry results as well as 1D and 2D 1H and 13C NMR analysis. The results of the in vitro tests confirm the antituberculous activity of the synthesized compounds. Furthermore, 6a, which is isolated in good yield, is a promising substrate for a new class of rifamycin derivatives. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)