Synthesis of glucosylglycerol in salt-stressed cells of the cyanobacterium Microcystis firma

Hagemann, Martin; Erdmann, Norbert; Wittenburg, Eckard

doi:10.1007/bf00456704

Cited by 39 publications

(24 citation statements)

References 16 publications

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“…Rapid changes in levels of glucosylglycerol following exposure to hyperosmotic or hypoosmotic stress were reported previously, 33,34 but no significant turnover of glucosylglycerol was observed in saltacclimated cells. 35,36 Our results suggest that in addition to serving as a compatible solute glucosylglycerol may also have a function of an energy or carbon store.…”

Section: Resultsmentioning

confidence: 82%

Untargeted metabolic footprinting reveals a surprising breadth of metabolite uptake and release by Synechococcus sp. PCC 7002

2011

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Cyanobacteria are important primary producers in diverse ecosystems, yet little is known about the extent of their metabolic interactions with the environment. We have used an integrated, untargeted metabolic footprinting approach to systematically evaluate the uptake and release of metabolites between a model marine cyanobacterium Synechococcus sp. PCC 7002 and different growth media. It was found that 47 out of 202 detected metabolites were consumed, and an additional 55 metabolites were released by the cells. Surprisingly, Synechococcus was found to uptake a great diversity of metabolites dominant in and specific to its own metabolite extract including histidine betaine (hercynine), g-glutamyl phenylalanine and a hexosamine-based trisaccharide. This provides Synechococcus a mechanism to benefit from the lysis of part of their population (i.e. due to environmental stress or predation). Additionally, stable isotope probing was used to show that adenine and glutamate are actively metabolized following uptake. A significant turnover of glucosylglycerol, a cyanobacterial compatible solute, as opposed to a negligible turnover of the hexosamine-based trisaccharide were also observed using stable isotope probing. The untargeted metabolic footprinting approach used in this study is generally applicable to investigate metabolic interactions of microorganisms with the environment and may prove useful to construct microbial community foodwebs.

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Section: Resultsmentioning

confidence: 82%

Untargeted metabolic footprinting reveals a surprising breadth of metabolite uptake and release by Synechococcus sp. PCC 7002

2011

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“…After the times indicated in the Figures samples were taken aseptically from the cultures and used in the labelling experiments. Cell number, cell volume and the content of glucosylglycerol were estimated as described by Hagemann et al (1987).…”

Section: Methodsmentioning

confidence: 99%

Alterations of protein synthesis in the cyanobacterium Synechocystis sp. PCC 6803 after a salt shock

Hagemann

Wölfel

Krüger

1990

Journal of General Microbiology

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Salt-induced changes in protein synthesis were investigated in the cyanobacterium Synechcystis sp. PCC 6803. Immediately after a salt shock of 684 mM-NaC1, total protein synthesis was almost completely blocked. Then, parallel to the accumulation of the osmoprotective compound glucosylglycerol, protein synthesis recovered gradually but remained diminished. The activation of glucosylglycerol synthesis was not inhibited by chloramphenicol at concentrations which totally inhibited protein synthesis. The qualitative protein composition of salt-shocked and control cells was similar. However, the rates of synthesis of single proteins were altered in cells shocked for 10 h and adapted to high salt conditions. Using two-dimensional gel electrophoresis, proteins were found which were synthesized at enhanced rates after adding salt.

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“…Adjustment of the internal osmotic pressure with organic solutes has been reported for a wide range of microorganisms, including bacteria (Csonka, 1989), cyanobacteria (Reed et al, 1986;Hagemann et al, 1987), fungi (Gadd et al, 1984;Jennings, 1984;Hocking, 1986), algae (Ben-Amotz & Avron, 1983) and yeasts (Adler et al, 1985 ;Meikle et al, 1988). Osmotically stressed yeasts accumulate osmoregulatory solutes such as glycerol and arabitol (Onishi, 1963).…”

Section: Introductionmentioning

confidence: 99%

Effect of salt stress on lipid composition and membrane fluidity of the salttolerant yeast Zygosaccharomyces rouxii

Hosono¹

1992

Journal of General Microbiology

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When the salt-tolerant yeast Zygosuccharomyces rouxii was grown in YPD medium containing 15% (w/v) NaCl, the relative amounts of C16:1 and c 1 8 : 1 fatty acids in acyl lipids increased and those of c18:O and c18:2 acids decreased both in whole cells and in crude plasma membrane preparations, as compared with cells grown in YPD medium alone. The proportions of C12:o and C14:o acids, which are minor components of yeast lipids, decreased in whole cells and markedly increased in plasma membranes when 15 % NaCl was included in the growth medium. The degree of unsaturation of fatty acids in the membranes and in whole cells decreased in the presence of 15% NaCl in the culture medium. The amount of free ergosterol in the membranes of cells grown in 15% NaCl increased to 2.9 times that of control cells. The ratio of ergosterol to phospholipid increased to 5 times that of control cells, whereas the ratio of phospholipid to protein in the membranes of cells grown in 15 % NaCl decreased to less than half that of control cells. The fluorescence polarization value of DPH (1,6-diphenyl-l,3,5-hexatriene) in membranes of cells grown in 15% NaCl was 1.2 times higher than that for membranes from control cells, indicating a decrease in membrane fluidity in the presence of a high concentration of NaCl.

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Synthesis of glucosylglycerol in salt-stressed cells of the cyanobacterium Microcystis firma

Cited by 39 publications

References 16 publications

Untargeted metabolic footprinting reveals a surprising breadth of metabolite uptake and release by Synechococcus sp. PCC 7002

Untargeted metabolic footprinting reveals a surprising breadth of metabolite uptake and release by Synechococcus sp. PCC 7002

Alterations of protein synthesis in the cyanobacterium Synechocystis sp. PCC 6803 after a salt shock

Effect of salt stress on lipid composition and membrane fluidity of the salttolerant yeast Zygosaccharomyces rouxii

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