Synthesis of food-grade 6-O-ascorbyl fatty esters and their semi-synthesis from low-value oils as resources

Pandey, Nishant; Singh, Mandeep; Dwivedi, Pratibha; Ahluwalia, Vivek; Sangwan, Rajender S.; Mishra, Bhuwan B.

doi:10.1007/s13399-021-01682-9

Cited by 4 publications

(1 citation statement)

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“…l -Ascorbic acid (AsA)-2-phosphate (AsA-2P) is a high-value-added derivative of AsA (also known as vitamin C) that exhibits antioxidant properties, is stable in aqueous solution and high-temperature conditions, and can be used to promote cell proliferation and prevent rough skin aging. − Similar to AsA, AsA-2P is widely used in foods, pharmaceuticals, and cosmetics. In recent decades, biocatalysis has emerged as a promising and environmentally friendly alternative to chemocatalysis for the synthesis of valuable compounds, including phosphorylation of AsA to AsA-2P. − …”

Section: Introductionmentioning

confidence: 99%

Enzymatic Production of Ascorbic Acid-2-Phosphate by Engineered Pseudomonas aeruginosa Acid Phosphatase

Song

Zheng

et al. 2021

J. Agric. Food Chem.

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l-Ascorbic acid-2-phosphate (AsA-2P) is stable in aqueous solution and at high temperatures and is widely used in foods, pharmaceuticals, cosmetics, and fodders; however, practical application of enzymatic synthesis methods to promote industrial-scale production of AsA-2P remains a major challenge. In this study, we enhanced the phosphorylation efficiency of Pseudomonas aeruginosa acid phosphatase (APase; EC 3.1.3.2) for AsA-2P production via protein engineering. Among the mutants obtained, we selected the most efficient mutant (Var5; G125E/D135T/S136N), which exhibited an increased k cat of 18.6 s–1 and a K m for AsA of 223.9 mM. In addition, Var5 exhibited a maximum enzyme activity of 2080.4 U/L after 10 h of fermentation, which was 80% higher than the wild-type enzyme. Furthermore, under optimal conditions, Var5 showed a maximal conversion of 48.6% and achieved a final AsA-2P titer of 61.5 g/L at 8 h, which is considerably higher than that reported for other similar biocatalytic approaches. These findings demonstrate the potential of this method for the large-scale production of AsA-2P.

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