Synthesis of Fluorescent Silica Nanoparticles and Their Applications as Fluorescence Probes

Song, Xu Chun; Li, Fang; Ma, Jingwei; Jia, Nengqin; Xu, Jianming; Shen, Hujun

doi:10.1007/s10895-010-0799-6

Cited by 27 publications

(20 citation statements)

References 26 publications

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“…Although the Stöber method can be utilised to obtain particles with a narrow size distribution in the range of 100 nm to several micrometres, the method is less suited for the synthesis of silica nanoparticles with a narrow dispersity in the size regime below 100 nm. Thus many research studies have been based on this approach, developing new synthesis methods of fluorescence silica nanoparticles [36–38]. One of the most outstanding methods was developed by Wiesner et al, who designed new small fluorescent core–shell silica nanoparticles [39–41].…”

Section: Introductionmentioning

confidence: 99%

Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy

Tavernaro

Cavelius²,

Peuschel

et al. 2017

Beilstein J. Nanotechnol.

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In recent years, fluorescent nanomaterials have gained high relevance in biological applications as probes for various fluorescence-based spectroscopy and imaging techniques. Among these materials, dye-doped silica nanoparticles have demonstrated a high potential to overcome the limitations presented by conventional organic dyes such as high photobleaching, low stability and limited fluorescence intensity. In the present work we describe an effective approach for the preparation of fluorescent silica nanoparticles in the size range between 15 and 80 nm based on L-arginine-controlled hydrolysis of tetraethoxysilane in a biphasic cyclohexane–water system. Commercially available far-red fluorescent dyes (Atto647N, Abberior STAR 635, Dy-647, Dy-648 and Dy-649) were embedded covalently into the particle matrix, which was achieved by aminosilane coupling. The physical particle attributes (particle size, dispersion, degree of agglomeration and stability) and the fluorescence properties of the obtained particles were compared to particles from commonly known synthesis methods. As a result, the spectroscopic characteristics of the presented monodisperse dye-doped silica nanoparticles were similar to those of the free uncoupled dyes, but indicate a much higher photostability and brightness. As revealed by dynamic light scattering and ζ-potential measurements, all particle suspensions were stable in water and cell culture medium. In addition, uptake studies on A549 cells were performed, using confocal and stimulated emission depletion (STED) microscopy. Our approach allows for a step-by-step formation of dye-doped silica nanoparticles in the form of dye-incorporated spheres, which can be used as versatile fluorescent probes in confocal and STED imaging.

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Section: Introductionmentioning

confidence: 99%

Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy

Tavernaro

Cavelius²,

Peuschel

et al. 2017

Beilstein J. Nanotechnol.

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“…Coupling to specifi c biomolecules Not reported Possible [53] Relatively easy bio-conjugation [34,54,55] Cell penetration Easy a) [21] Easy [56] More diffi cult without biomolecule [54] Toxicity Dependent on amount of surfactant and material a) [21] Dependent on amount of surfactant and fl uorophore [57] Very low [58,59] Nonlinear optical properties Not reported σ TPA ≈ 100-2000 GM [53,60] σ TPA ≈ 217-1200 GM [61] …”

Section: Structurementioning

confidence: 99%

Two‐Photon Imaging of a Cellular Line Using Organic Fluorescent Nanoparticles Synthesized by Laser Ablation

Aparicio-Ixta

Alba-Rosales

Ramos‐Ortíz

et al. 2016

Part & Part Syst Charact

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A comparative study of the optical properties of organic fl uorescent nanoparticles fabricated by laser ablation (NPs-LA), reprecipitation (NPs-RP), and microemulsion (NPs-ME) methods is presented. These nanoparticles contain a fl uorene-based p-conjugated molecule (BT2). Distinctive electronic transitions are observed in samples due to the specifi c way in which the molecule BT2 is assembled in each type of nanoparticles; for instance, transitions involved in absorption and emission spectra of NPs-LA result in blueshifting with respect to the molecular solution of BT2, whereas redshifting is observed in NPs-RP and NPs-ME. Further, the results show that under infrared excitation, the aqueous suspensions of NPs-LA exhibit the highest fl uorescence induced by two-photon absorption (≈790 GM at 740 nm), as well as the best photostability, compared with aqueous suspensions of NPs-RP and NPs-ME. The nanoparticles synthetized by the three aforementioned methods are employed as exogenous agents for the visualization of human cervical cancer cell line (HeLa) using confocal and two-photon microscopy. Under similar experimental conditions, it is found that microscopy images of the best quality are obtained with NPs-LA. These results show that laser ablation is a suitable technique for the fabrication of organic fl uorescent nanoparticles used as contrast agents for in vitro fl uorescence microscopy.O-NPs have been successfully exploited as contrast agents (fl uorescent markers) for in vitro and in vivo studies in cellular lines and organisms, [ 5 ] medical imaging, [ 6 ] diagnostic purposes, [ 7 ] and positive confi rmation of the site-specifi c delivery of a drug with the goal of obtaining effective treatment of diseases. [ 8,9 ] Some advantages obtained by using organic nanostructures are: i) stability of fl uorescent organic materials in aqueous suspensions, ii) reduced toxicity, iii) decreased degradation, and iv) selectivity toward specifi c targets.Simple and versatile strategies of fabrication have been used to obtain O-NPs with different morphologies and optical properties in aqueous media. Reprecipitation (RP), [ 10 ] self-assembly, [ 11 ] ion-association, [ 12 ] condensation, [ 13 ] microemulsion (ME), [ 14 ] and laser ablation (LA) [ 15 ] are some methods used to fabricate O-NPs. For the preparation of O-NPs comprising luminescent properties, the most commonly employed methods are RP and ME. [ 16,17 ] LA has also been used to obtain fl uorescent O-NPs, [18][19][20] and some basic characterization of their optical properties has been performed, but to the best of our knowledge, demonstration of their use in the context of biophotonic applications, i.e., imaging of biological samples, has

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“…There are two advised techniques to create dye-doped SiNP: The reverse microemulsion method 2,28,29 and the Stober's sol-gel method. 76,77 SiNPs are made fluorescent through a process known as dye-doping, 78 which involves incorporating fluorescent dyes inside the matrix of the NP. In order to obtain maximum fluorescence, it is important to find the optimal amount of dye to be incorporated.…”

Section: Overviewmentioning

confidence: 99%

Fluorescent Silica Nanoparticles for Medical Imaging

Tharkur¹,

Ricaurte²,

Santra³

2014

Frontiers in Nanobiomedical Research

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Synthesis of Fluorescent Silica Nanoparticles and Their Applications as Fluorescence Probes

Cited by 27 publications

References 26 publications

Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy

Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy

Two‐Photon Imaging of a Cellular Line Using Organic Fluorescent Nanoparticles Synthesized by Laser Ablation

Fluorescent Silica Nanoparticles for Medical Imaging

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