Synthesis of electrochemical probes for nucleic acid detection

Metal–Carbon Bonds in Biopolymer Conjugates: Bioorganometallic Nucleic Acid Chemistry

“…To improve the sensitivity of the system as an electrochemical DNA sensor, up to eight ferrocene monomers have recently been oligomerized into a single hairpin strand of DNA. 67 Ihara and co-workers also incorporated ferrocene into a DNA hairpin using the same 19 and longer 20 polyethylene Figure 7. Ferrocene-functionalized triphosphates for enzymatic incorporation into DNA.…”

Methylene blue phosphoramidite for DNA labelling

“…Bis-propanol ferrocene phosphoramidite has proved to be efficient in incorporating numerous redox markers in the sequence. [42][43][44][45] Since many dyes are unstable under strong basic conditions, labelled DNA has to be deprotected under a mild basic treatment to ensure its integrity. It is worth noting that MB is not stable in these mild basic conditions and the molecule needs to be chemically modified to improve its stability.…”

Electrochemical detection of nucleic acids using pentaferrocenyl phosphoramidate α-oligonucleotides

“…The electrochemical characterization of each Fc 5 -a-DNA was achieved by cyclic voltammetry (CV) on a gold electrode microarray. 27 This electrochemical biochip consisted of a plastic-based support with eight working electrodes specially designed to be a low cost, low density biodevice. Measurements were made in a 10 mL droplet of probe solution in a phosphate buffer.…”

“…In our laboratory, we focused on developing multiferrocenyl hairpin probes which offer significant improvement of detection limit. [27][28][29] We have synthesized a ferrocene bispropanol phosphoramidite synthon that can be incorporated several times in an oligonucleotide sequence, directly during DNA solid-phase synthesis. By this strategy, a multilabel tag can be bound to a DNA probe.…”