Synthesis of Cellulose by Resting Cells of Acetobacter xylinum

Hestrin, S.; Aschner, M.; Mager, J.

doi:10.1038/159064a0

Cited by 73 publications

(40 citation statements)

References 8 publications

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“…The thickening of the pellicle Synthesis of cellulose from glucose by a suspension of non-proliferating bacteria is a function of oxygen supply (Hestrin et al 1947). The rate of cellulose production was negligible under nitrogen, about one-half optimal under air and approached closely the optimum rate under 100% oxygen in a reciprocally shaken suspension of cells in glucose solution.…”

Section: Resultsmentioning

confidence: 99%

“…The understanding of the morphogenesis of this remarkable structure is relevant to efficient technical use of A , xylinum as an oxidizing organism and as a cellulose source (Evans & Hibbert, 1947) and to the use of the organism as a model system in which physical and chemical aspects of cell-wall production can be examined outside the cell (Hestrin, Aschner & Mager, 1947; Muhlethaler, 1948Muhlethaler, , 1949Hestrin, 1953; Hestrin . In the present communication the mechanics of the flotation and thickening of a xylinum pellicle are considered and the effects of agitation on the expression of the cellulose-synthesizing activity and its heritable transmission are described.…”

mentioning

confidence: 99%

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Factors affecting Production of Cellulose at the Air/ Liquid Interface of a Culture of Acetobacter xylinum

Schramm

Hestrin

1954

Journal of General Microbiology

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333

168

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Factors affecting Production of Cellulose at the Air/ Liquid Interface of a Culture of Acetobacter xylinum

Schramm

Hestrin

1954

Journal of General Microbiology

Self Cite

333

168

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“…To produce the bacterial cellulose, Acetobacter xylinum ATCC 23768 was cultured in the SH medium containing glucose, 2%(w/v) ; Peptone, 0.5% (w/v); yeast extract,0.5%(w/v); disodium phosphate,0.27% (w/v);citric acid,0.115 (w/v); at pH 6.0 and under static culture condition [11]. The cellulose sheets after cultivation were removed and rinsed with distilled water and devoided from bacterial and medium residues by 2% SDS (Sodium Dodecyl Sulfate) and %4 NaOH solutions in a bath of water at boiled temperature [12].…”

Section: Methodsmentioning

confidence: 99%

Removal of Mercury from chlor-alkali Industry Wastewater using Acetobacter xylinum Cellulose

Rezaee¹,

Derayat²,

Mortazavi³

et al. 2005

American J. of Environmental Sciences

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Abstract:In this study, the removal of mercury ions by cellulose of Acetobacter xylinum was investigated in the synthetic and chlor-alkali wastewater. Biofilms of Acetobacter xylinum were grown in laboratory column bioreactors. The biofilms were continuously treated with sterile synthetic model wastewater or nonsterile, neutralized chloralkali wastewater.The extent of adsorption was studied as function of pH, adsorbent dose and contact time. Efficiency of mercury ion removal from chlor-alkali industry wastewater by aluminum sulfate and ferric chloride was also determined. Under acidic condition the adsorption of mercury by cellulose was quite low and increasing processing time more than10 min has no remarkably effect on the adsorption rate. Adsorption capacity of cellulose under dynamic condition for chlor-alkali wastewater was 65µg/g which was less than the value (80µg/g) that obtained from batch adsorption experiments for synthetic wastewater.

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“…xylinum (ATCC 53524) in Hestrin Schramm medium pH 6.2 (Hestrin and Schramm, 1954) containing 2% glucose or experimental incubations in medium containing 2% glucose and 0.5% tamarind xyloglucan purified from Glyloid 3S (Dainippon Pharmaceutical Co., Osaka, Japan, GIc:XyhGal 2.8:2.25:1; Gidley etaL, 1991) or carboxymethylcellulose (CMC, 4M6F, Hercules) were performed at 30°C and 50 r.p.m. After 48 or 72 h incubation, cellulosic material was removed aseptically and washed extensively in sterilized deionized water at room temperature.…”

Section: Bacterial Culturesmentioning

confidence: 99%

In vitro assembly of cellulose/xyloglucan networks: ultrastructural and molecular aspects

et al. 1995

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SummaryFeatures of the interaction between cellulose and xyloglucen have been studied using the cellulose-producing bacterium Acetobacter aceti ssp. xylinum (ATCC 53524) and tamarind seed xyloglucen. Direct microscopic evidence is provided for the generation of cross-bridges between cellulose ribbons produced in the presence of xyloglucan but not cerboxymathyiceltulose. Cross-bridge lengths are very similar to those observed for de-pectinated onion cell walls. Similar cross-bridge lengths are observed following mixing of isolated A. xylinum cellulose and xyloglucan, showing that network formation can be an abiotic process. The level of incorporation of xyloglucan in an actively growing system (ca. 38% of cellulose) is an order of magnitude higher than that observed in mixtures of isolated polymers and is comparable with cell wall levels.NMR spectroscopy suggests that 80-85% of incorporated xyloglucen is segmentally rigid with the backbone adopting an extended 'cellulosic' conformation and probably aligned with cellulose chains. The remaining xyioglucan is more mobile and is assigned to cross-bridges with, on average, a twisted backbone conformation. No evidence for specific involvement of side-chain residues in binding is found, and the observation of cross-bridges with a non-fucosylated xyloglucan shows that fucose residues are not essential for network formation. Xyloglucen causes cellulose ribbons to become more amorphous end to have a decreased IoJl[~ crystallite ratio without any significant alteration in ribbon diameter.Based on the findings that levels of xyloglucan incorporation, the presence and lengths of cross-bridges, and the modification of cellulosic molecular organization are all similar to those found in plant cell walls, we suggest that

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Synthesis of Cellulose by Resting Cells of Acetobacter xylinum

Cited by 73 publications

References 8 publications

Factors affecting Production of Cellulose at the Air/ Liquid Interface of a Culture of Acetobacter xylinum

Factors affecting Production of Cellulose at the Air/ Liquid Interface of a Culture of Acetobacter xylinum

Removal of Mercury from chlor-alkali Industry Wastewater using Acetobacter xylinum Cellulose

In vitro assembly of cellulose/xyloglucan networks: ultrastructural and molecular aspects

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