Synthesis of bis(ylidene) cyclohexanones and their antifungal activity against selected plant pathogenic fungi

Silva, Ueveton Pimentel da; Ferreira, Bruno; Sousa, Bianca Lana de; Barreto, Robert W.; Martins, Felipe Terra; Neto, João Honorato de Araújo; Vaz, Boniek Gontijo; Silva, Rodolfo Rodrigues da; Martins, Thaís Viana Fialho; Mendes, Tiago; Varejão, Eduardo Vinícius Vieira

doi:10.1007/s11030-022-10431-7

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“…In order to evaluate the toxicity of extract, the colorimetric assay of MTT (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used to determine the cytotoxic concentration that decreases cell viability by 50% (CC 50 ) from cell cultures of African green monkey kidney epithelial Vero (ATCC ® CCL-81™), liver epithelial-like HEPG2 (ATCC ® HB-8065™), human kidney embryo HEK-293 (ATCC ® CRL-1573™), mouse macrophage RAW 264.7 (ATCC ® TIB-71™), and rat myoblast L6 (ATCC ® CRL-1458™) [ 45 ]. A suspension of each cell type, containing approximately 1 × 10 4 cells/mL, was seeded in a 96-well plate (100 µL/well) until reaching 70–80% confluence.…”

Section: Methodsmentioning

confidence: 99%

Hibiscus acetosella: An Unconventional Alternative Edible Flower Rich in Bioactive Compounds

et al. 2023

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The interest in the consumption of edible flowers has increased since they represent a rich source of bioactive compounds, which are significantly beneficial to human health. The objective of this research was to access the bioactive compounds and antioxidant and cytotoxic properties of unconventional alternative edible flowers of Hibiscus acetosella Welw. Ex Hiern. The edible flowers presented pH value of 2.8 ± 0.00, soluble solids content of 3.4 ± 0.0 °Brix, high moisture content of about 91.8 ± 0.3%, carbohydrates (6.9 ± 1.2%), lipids (0.90 ± 0.17%), ashes (0.4 ± 0.0%), and not detectable protein. The evaluation of the scavenging activity of free radicals, such as 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), of the flower extract was better than the results observed for other edible flowers (507.8 ± 2.7 μM TE and 783.9 ± 30.8 μM TE, respectively) as well as the total phenolic composition (TPC) value (568.8 ± 0.8 mg GAE/g). These flowers are rich in organic acids and phenolic compounds, mainly myricetin, and quercetin derivatives, kaempferol, and anthocyanins. The extract showed no cytotoxicity for the cell lineages used, suggesting that the extract has no directly harmful effects to cells. The important bioactive compound identified in this study makes this flower especially relevant in the healthy food area due to its nutraceutical potential without showing cytotoxicity.

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Section: Methodsmentioning

confidence: 99%