Synthesis of Biogenic Silver Nanoparticles with Eco-Friendly Processes Using Ganoderma lucidum Extract and Evaluation of Their Theranostic Applications

Abstract

The green synthesis of silver nanoparticles (AgNPs) using plant extract, the cost-effective solution, and the abundance and environmental issue have been gaining much attention to scientists. Ganoderma lucidum (GL) commonly known as Lingzhi in Chinese and Reishi in Japanese, with a proven anticancer benefit, is discovered in the buffer zone of Bach Ma National Park, Nam Dong district, Thua Thien Hue province. In this work, the AgNPs were synthesized in a simple and effective biochemical reduction process using GL which is one of the biological organisms, as a reducing and stable agent. The optimum conditions of various experimental parameters such as pH, reaction time, concentration, and temperature were investigated. Obtained AgNPs were characterized by UV-Vis, FTIR, SEM, energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The effects of AgNP/GL materials and GL aqueous extraction on the antiproliferative activities of HepG2 and MCF-7 cells were studied. The novel AgNP/GL-based multicomponent suspension is a key compound that could find a good application in the medical and pharmaceutical sciences.

Optimized Synthesis of Small and Stable Silver Nanoparticles Using Intracellular and Extracellular Components of Fungi: An Alternative for Bacterial Inhibition

“…Synthesis of metallic NPs using G. sessile has not been previously reported; therefore, the optimized protocol used for T. harzianum was applied. A similar protocol was used to synthesize NPs using G. lucidum by [ 32 ], who reported AgNPs of 10.72 nm using the supernatant of fruiting bodies. The authors described as optimum conditions the use of 1 mM AgNO 3 and a reaction temperature of 85 °C.…”

“…However, most studies for the synthesis of AgNPs using Ganoderma spp. have been carried out with G. lucidum [ 32 , 42 , 43 , 45 , 46 , 47 ]. Strong bactericidal activity against S. aureus , E. coli , Staphylococcus mutants, Klebsilla pneumoniae and P. aeruginosa was reported using AgNPs (5–30 nm) synthesized with the supernatant of G. lucidum [ 42 ].…”

Synthesis, Characterization and Biomedical Application of Silver Nanoparticles

“…Nguyen and colleagues [286] reported on the treatment of hepatocellular carcinoma (HepG2) and breast cancer (MCF-7) with AgNPs synthesized in the presence of Ganoderma lucidum (GL). Additionally, they demonstrated that spherical nanoparticles of AgNPs/GL with a physical size of 10.72 nm effectively inhibited the proliferation of HepG2 and MCF-7 cells.…”

Mycology-Nanotechnology Interface: Applications in Medicine and Cosmetology

“…contain reducing and capping agents that are responsible for the synthesis of these Ag-NPs. 71 The list of different fungal species used to synthesize different nanoparticles have been comprehended in Table 1 .…”